Gilson PIPETMAN P Manuel utilisateur

PIPETMAN

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ENGLISH

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1 - INTRODUCTION

Congratulations on acquiring your new

Pipetman P. Enjoy the following features.

- Continuously-adjustable, designed to dis-

pense precise volumes of liquid safely.

CONTENTS page

1 - INTRODUCTION 2

2 - PARTS CHECK LIST 3

3 - DESCRIPTION 4

4 - OPERATING RANGES 5

5 - SETTING THE VOLUME 5

6 - PIPETTING 7

7 - GILSON DIAMOND

®

TIPS 10

8 - PERSONALIZING YOUR PIPETTE 12

9 - GLP FEATURES 13

10 - TROUBLESHOOTING 13

11 - LEAK TEST 15

12 - MAINTENANCE 16

13 - CLEANING AND DECONTAMINATION 20

14 - SPECIFICATIONS 22

15 - SPARE PARTS 24

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- Large range of volumes, eight models cover

a range from 0.1 µL to 10 mL, for many

applications:

P2 and P10: measurement and transfer of

micro-volumes, DNA sequencing and enzyme-

assays.

P20, P100, P200, P1000: measurement and

transfer of general aqueous solutions, acids

and bases.

P5000, P10ml: measurement and transfer of

large volumes.

- Volume is continuously adjustable within the

useful volume range using the digital

volumeter.

- Tip-ejector and disposable tips mean no

need to handle tips, protecting the user from

contamination.

- Carry-over between samples eliminated by

using a new tip from a Tipack™ box for each

transfer.

- Nominal volume (i.e. pipette model) is shown

on the color coded push-button.

- Personalize your Pipetman by fitting a

Coloris™ identification clip to the connecting

nut (5 colors).

2 - PARTS CHECK LIST

Just take a moment to verify that the following

items are present:

- Pipetman P,

- User's Guide,

- Sample pack of tips,

- Safety bag,

- Adhesive id-tags (strip of 4),

- Certificate of conformity (including bar-code

sticker).

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3 - DESCRIPTION

A) Color coded push-button for setting the

volume, aspirating, and dispensing.

B) Body or handle.

C) Connecting nut to upper part (body or

handle) of removable tip-holder.

D) Diamond tip.

E) Tip-ejector.

F) Tip-ejector button.

H) Tip-holder (removable for cleaning and

decontaminating).

T) Thumbwheel for setting the volume.

V) Volumeter.

A

T

B

V

F

C

E

H

D

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Push-button

Thumbwheel

4 - OPERATING RANGES

Model Range

P2 0.1 - 2 µL

P10 0.5 - 10 µL

P20 2 - 20 µL

P100 20 - 100 µL

P200 30 - 200 µL

P1000 200 - 1000 µL

P5000 1 - 5 mL

P10ml 1 - 10 mL

5 - SETTING THE VOLUME

The volume of liquid to be aspirated is set using

the volumeter. The volumeter consists of three

number-dials, which are read from top (most

significant digit) to bottom (least significant

digit). A marker is used to set exact or

intermediate volumes using the scale on the

bottom dial. The dials are colored either black

or red to indicate the position of the decimal

point, according to the model (see examples).

The volume is set by

turning the thumbwheel

(T) or the push-button (A).

The push-button makes it

easier and quicker to set

volumes, especially when

wearing gloves. The thumb-

wheel may be turned to

slowly reach the required

setting.

To obtain maximum

accuracy when setting the

volume, proceed as follows:

- when decreasing the volume setting, slowly

reach the required setting, making sure not

to overshoot the mark.

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- when increasing the volume setting, pass

the required value by 1/3 of a turn and then

slowly decrease the volume to reach the

volume, making sure not to overshoot the

mark.

Model Color of volumeter numbers

Black Red

P2 to P200 µL 0.1 µL and 0.01 µL

P1000, P5000 0.1 mL and 0.01 mL mL

P10ml mL 0.1 mL

Example for each pipette:

To avoid parallax errors, make sure that the

volume indicator and the selected volume

marking are in your direct line of vision. At close

range you may find it helps to close one eye.

0

7

5

7.5 µL

P10

1

2

5

1.25 µL

P2

1

2

5

12.5 µL

P20

0

7

5

75 µL

P100

0

7

5

7.5 mL

P10ml

1

2

5

125 µL

P200

0

7

5

0.75 mL

P1000

1

2

5

1.25 mL

P5000

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☞

With a precise pipetting technique P2 may be

used to aspirate volumes down to 0.1 µL, P10

at 0.5 µL, and P200 at 30 µL.

Volatile Solvents

For volatile solvents you should saturate the

air-cushion of your pipette by aspirating and

dispensing the solvent repeatedly before

aspirating the sample.

6 - PIPETTING

1) Fit a new Gilson Diamond Tip.

Plastic tips are for a single application - they

must not be cleaned for reuse. Push the tip-

holder into the tip using a slight twisting

motion to ensure a firm and airtight seal.

(1) For P2 and P10: tip ejector extensions

are supplied.

(2) P5000 and P10ml: insert a filter into the

tip holder before fitting a tip. (If the filter

gets dirty it should be replaced with a

clean one.)

(3) P5000 and P10ml: these models are not

equipped with tip-ejectors.

2) Pre-rinse the tip.

Some liquids (e.g. protein-containing solu-

tions and organic solvents) can leave a film

of liquid on the inside the wall of the tip; pre-

rinse the tip to minimize any errors that may

be related to this phenomenon.

Pre-rinsing consists of aspirating the first

volume of liquid and then dispensing it back

into the same vessel (or to waste). Subsequent

volumes that you pipette will have levels of

accuracy and precision within specifications.

3) Aspirate.

Press the push-button to the first stop (this

corresponds to the set volume of liquid).

Hold the pipette vertically and immerse the tip

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in the liquid (see immersion depth table).

Release the push-button slowly and

smoothly (to top position) to aspirate the set

volume of liquid.

Wait one second (time depends on model,

see table); then withdraw the pipette-tip

from the liquid.

You may wipe any droplets away from the

outside of the tip using a medical wipe,

however if you do so take care to avoid

touching the tip’s orifice.

4) Dispense.

Place the end of the tip against the inside

wall of the recipient vessel (at an angle of

10° to 40°).

Press the push-button slowly and smoothly

to the first stop.

Wait for at least a second; then press the

push-button to the second stop to expel

any residual liquid from the tip.

Keep the push-button pressed fully down

and (while removing the pipette) draw the

tip along the inside surface of the vessel.

Release the push-button, smoothly.

Top

1st Stop

2nd Stop

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5) Eject the tip by pressing firmly on the tip-

ejector button.

General Guidelines for Good Pipetting

1) Make sure that you operate the push-button

slowly and smoothly.

2) When aspirating, keep the tip at a constant

depth below the surface of the liquid (refer

to the table).

Table - Immersion Depth & Wait Time

Model Immersion Wait Time

Depth (mm) (Seconds)

P2 1 1

P10 1 1

P20 2-3 1

P100 2-4 1

P200 2-4 1

P1000 2-4 2-3

P5000 3-6 4-5

P10ml 5-7 4-5

3) Change the tip before aspirating a different

liquid, sample, or reagent.

4) Change the tip if a droplet remains at the

end of the tip from the previous pipetting

operation.

5) Each new tip should be pre-rinsed with the

liquid to be pipetted.

6) Liquid should never enter the tip-holder; to

prevent this:

- press and release the push-button slowly

and smoothly,

- never turn the pipette upside down,

- never lay the pipette on its side when there

is liquid in the tip.

7) When pipetting liquids with temperatures

different to the ambient temperature, pre-

rinse the tip several times before use.

8) You may remove the tip-ejector (see “Main-

tenance”) to aspirate from very narrow tubes.

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9) After pipetting acids or other corrosive

liquids that emit vapors, remove the tip-

holder and rinse the piston and O-ring and

seal with distilled water.

10) Do not pipette liquids having temperatures

above 70 °C or below 4 °C.

The pipette can be used between + 4 °C

and + 40 °C but the specifications may

vary according to the temperature (see

Chapter 14 for controlled conditions of

use).

7 - GILSON DIAMOND TIPS

Gilson Diamond Tips are made to the highest

specifications, strict quality control is

maintained throughout the manufacturing

process.

Diamond Tips are used to calibrate Pipetman P,

therefore for optimum performance, you are

strongly advised to use Gilson's Diamond Tips

with your Pipetman P. Diamond Tips have the

Gilson logo engraved on their collar, ensuring

that you have a genuine Gilson product.

To ensure accuracy and precision, Gilson's

Quality Assurance System focuses on the

following critical parameters.

• Diamond Tips are made from pure polypro-

pylene (virgin, metal-free, to avoid the possibility

of contamination). They are available

sterilized.

• Optimized shape (revised collar for optimum

sealing, thin walls, and fine point), making

them easier to mount, more flexible, with no

vortexing, and improved precision.

• Diamond Tips are free from even microscopic

defects, especially at the orifice. All surfaces

are smooth and hydrophobic, thereby avoid-

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ing the excessive retention of liquids that

causes poor accuracy and a lack of precision.

• Mold and cavity references

are marked on the collar,

ensuring the traceability

for quality assurance

purposes, batch numbers

appear on all packages

(bags and boxes).

• They form an air-tight seal with the tip-holder,

preventing the leaks that cause poor accuracy

and a lack of precision.

• They may be autoclaved at 121°C for 20

minutes at 0.1 MPa.

Diamond Filter Tips

Filter tips are used when sample-to-sample,

sample-to-pipette, or sample-to-operator con-

tamination must be avoided.

Because of there unique construction, Diamond

Filter (DF) Tips may be autoclaved, without risk

of damage to the filters. Gilson's sterilized

Diamond Filter Tips are certified free of detect-

able RNase, DNase, DNA, RNA, and protease.

Table - Tips to Use for Best Results

Gilson

Logo

ID Number

Pipette Diamond Tips Volume (Range)

P2 D10, DL10, DF10, DFL10 0.1 µL to 2 µL

P10 D10, DL10, DF10, DFL10 0.5 µL to 10 µL

P20 DF 30, D200 2 µL to 20 µL

DF30 20 µL to 30 µL

D200, DF100 20 µL to 100 µL

DF100 20 µL to 100 µL

D200, DF200, D300, DF300 20 µL to 200 µL

P1000 D1000, DF1000 200 µL to 1 mL

P5000 D5000 1 mL to 5 mL

P10 ml D10 ml 1 mL to 10 mL

P200

P100

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8 - PERSONALIZING YOUR PIPETTE

Personal Label - Application Name Tag

In addition to the

four round adhesive

stickers, supplied

with your pipette,

you can identify

your pipette with a

Coloris clip. Made

of polyacetal, these

clips accept perma-

nent ink, and wipe

clean with ethanol.

Ideal for recording service or calibration dates.

Perfect for quick visual identification. Also useful

for identifying pipettes dedicated to specific

applications or as a warning when there is risk

of contamination.

These brightly colored clips snap quickly over

the connecting nut of Pipetman models P2 to

P1000. Because they don’t require dismantling

or changing original parts, there’s no danger of

the pipette’s calibration being affected.

Tip-ejector: Dual-position Adaptor for P2

and P10

The component parts of a tip-ejector assembly

are a metallic rod and a blue plastic connector,

which goes into the body of the pipette.

For P2 and P10, a

dual-position adaptor

(plastic) is also

required; the metallic

rod is shaped so that

the adaptor may be

clipped to it in either of

two positions.

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Pipetman P2 and P10 are delivered with the

adaptor in place and configured to accept

DL10 tips. When you use D10 tips, which are

shorter, you must reposition the adaptor as

follows.

1) Pull the adaptor away from the metallic rod.

2) Turn the adaptor through 180°.

3) Refit the adaptor so that the end of the

metallic rod engages the shorter slot of the

adaptor.

4) Finally, check that end of the metallic rod is

correctly seated in the appropriate slot of

the adaptor; the ‘dimple’ on the metallic rod

must be engaged in the corresponding

hole on the adaptor.

9 - GLP FEATURES

These are as follows:

• Serial Number:

engraved on body

of the pipette.

• Bar Code:

on the box and with

the certificate (can

be transferred).

• ID Tag (Application

or User).

10 - TROUBLESHOOTING

You may be able to identify and to correct the

problem by reference to the following table.

If you can’t solve the problem, contact your

Gilson representative.

However, a quick inspection of the pipette may

help you isolate the problem.

ID Tag

Serial Number

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☞

Is the operating rod straight?

Is the operating rod free of chemical damage?

Are the volumeter digits clear and easy to

read?

Can you set any volume in the useful range?

Can you set the nominal (maximum) volume?

Does the volumeter operate smoothly without

hitching?

If the answer to any of these questions is NO,

it is probable the pipette has been damaged as

the result of a mechanical shock or chemical

damage, in which case it should be returned to

your supplier for repair.

If the answer to these questions is YES, conti-

nue as follows.

Is the tip-ejector straight?

Is the tip-ejector free of chemical damage?

Remove tip-ejector; is the tip-holder undamaged

and free of chemical damage?

Are the O-ring and seal undamaged and free

of chemical damage?

If answer is NO, clean or replace the damaged

part.

If the answer is YES, refer to the table on page 15;

you may need to clean or replace the piston

assembly, O-ring and seal. Pipetman must be

adjusted (recalibrated) after the piston

assembly is changed.

If you see a bubble in the aspirated liquid:

dispense the liquid into its original vessel, then

ensure that the tip is properly immersed below

the surface of the liquid and aspirate more

slowly - if a bubble appears again, replace the

tip.

Before returning any pipette, ensure

that it is completely free of chemical,

biological, or radioactive contamination.

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Symptom Possible Cause Action

Pipette is leaking sample Worn O-ring or seal. Replace both parts.

Pipette won’t aspirate Worn O-ring or seal. Replace both parts.

Tip holder is loose. Tighten connecting nut.

Piston is damaged (chemically or mechanically). Return pipette to supplier.

Damaged tip holder. Replace the tip-holder.

Connecting nut is loose. Tighten connecting nut.

Pipette is inaccurate Improper assembly. See 'Maintenance'.

Unscrewed tip-holder. Tighten connecting nut.

Connecting nut is loose. Tighten connecting nut.

Pipette is not precise Tip-holder is loose. Tighten connecting nut.

Incorrect operator technique. Operator training.

Worn O-ring or seal. Replace both parts.

Connecting nut is loose. Tighten connecting nut.

Piston is damaged (chemically or mechanically). Return pipette to supplier.

Damaged tip-holder. Replace the tip-holder.

Tips fall off or don’t fit Low quality tips. Use Gilson Diamond Tips.

Damaged tip-holder. Replace the tip-holder.

Damaged tip-ejector. Replace the tip-ejector.

11 - LEAK TEST

This test may be performed at any time to check

that the pipette does not leak, especially after

performing a maintenance or decontamination

procedure. If a pipette fails this test you should

replace the O-ring and seal and repeat this test,

after making sure that the pipette is correctly

reassembled.

P2 to P200

- fit a Gilson Diamond Tip,

- set the pipette to the maximum volume given

in the specifications, and pre-rinse,

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- aspirate the set volume from a beaker of

distilled water,

- maintain the pipette in the vertical position

and wait for 20 seconds,

- if a water droplet appears at the end of the tip

there is a leak,

- if you see no droplet, re-immerse the tip below

the surface of water,

- the water level inside the tip should remain

constant; if the level goes down there is a leak.

P1000 to P10ml

- fit a Gilson Diamond tip,

- set the pipette to the maximum volume given

in the specifications,

- aspirate the set volume from a beaker of

distilled water,

- maintain the pipette in the vertical position

and wait for 20 seconds,

- if a water droplet appears at the end of the tip,

there is a leak.

12 - MAINTENANCE

Routine maintenance will help keep your pipette

in good condition, ensuring a continued high

level of performance. Maintenance is limited to

cleaning or autoclaving the parts specified

under “Cleaning and Decontamination” or to

replacing the push-button, connecting nut, tip-

ejector, tip-holder, seal and O-ring.

Pipetman P2 and P10 should not be dis-

assembled, so you may only replace the push-

button, tip-ejector, dual position tip-ejector and

its adapter; with these pipettes if the tip-holder

is damaged, the piston may also be damaged.

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A

P

C

E

H

S1

A) Color coded

push-button.

C) Connecting nut.

E) Tip-ejector.

H) Tip-holder.

P) Piston assembly.

S1) Seal.

S2) O-ring.

쒆

S2

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Tip-ejector and Tip-holder

These parts must be changed, if they are

accidentally damaged or attacked chemically.

You should also remove these parts for cleaning

or decontamination purposes. A cloth

dampened with ethanol may be used to

routinely clean the outside of the pipette.

Changing the Tip-ejector

1) To remove the tip-ejector, keep the tip-

ejector button depressed and pull down on

the flanged upper part of the tip-ejector with

the other hand (moderate force is required).

2) To refit the tip-ejector, keep the tip-ejector

button depressed, slide the end of the tip-

ejector over the end of the tip-holder and

push the plastic end of the tip-ejector back

into the body of the pipette until it is gripped

firmly by the tip-ejector rod (metal).

Changing the Tip-holder

1) Remove the tip-ejector (see above).

2) Unscrew the connecting nut by turning it

counterclockwise, by hand.

3) Carefully separate the lower and upper parts.

4) Remove the piston assembly, O-ring and

seal.

5) Clean, autoclave, or replace the tip-holder.

6) Reassemble the pipette (refer to the figure).

7) Tighten the connecting nut (turn by hand,

clockwise).

8) Refit the tip-ejector (see above).

Servicing the Piston Assembly

You may remove the piston assembly for

cleaning purposes only. If the piston assembly

is changed, the pipette must be adjusted and

calibrated.

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The piston assembly must not be

autoclaved.

1) Remove the tip-ejector (see above).

2) Unscrew the connecting nut by turning it

counterclockwise, by hand.

3) Carefully separate the lower and upper

parts.

4) Remove the piston assembly, O-ring and

seal.

5) Clean and decontaminate the piston

assembly.

6 Reassemble the pipette (refer to the figure).

7) Tighten the connecting nut (turn by hand,

clockwise).

8) Refit the tip-ejector (see above).

Changing the O-ring

The O-ring and seal are normally to be found

on the piston; they must not be autoclaved, if

worn or damaged in any way (chemical or

mechanical), they must be replaced. The di-

mensions of the O-ring vary according to the

model of pipette.

1) Remove the tip-ejector (see above).

2) Unscrew the connecting nut by turning it

counterclockwise, by hand.

3) Carefully separate the lower and upper parts.

4) Remove the piston assembly, O-ring and

seal.

5) Clean or replace the seal then the O-ring.

6) Reassemble the pipette (refer to the figure

opposite).

7) Tighten the connecting nut (turn by hand,

clockwise).

8) Refit the tip-ejector (see above).

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13 - CLEANING AND DECONTAMINATION

Pipetman P is designed so that the parts

normally in contact with liquid contaminants,

can easily be cleaned and decontaminated.

However, because Pipetman P2 and P10

contain miniaturized parts, it is best not to

disassemble these pipettes yourself.

Liquid must never enter the upper part

(body) of any pipette.

Cleaning

The pipette must be cleaned, as described below,

before it is decontaminated. Soap solution is

recommended for cleaning Pipetman P.

External

1) Remove the tip-ejector .

2) Wipe the tip-ejector with a soft-cloth or lint-

free tissue impregnated with soap solution.

3) Wipe the entire pipette with a soft-cloth or lint-

free tissue impregnated with soap solution,

to remove all dirty marks. If the pipette is

very dirty, a brush with soft plastic bristles

may be used.

4) Wipe the entire pipette and the tip-ejector

with a soft cloth or lint-free tissue

impregnated with distilled water.

5) Refit the tip-ejector and allow the pipette to

dry.

Internal

The following components only can be im-

mersed in a cleaning solution: connecting nut,

tip-ejector, tip-holder, piston assembly, seal

and O-ring.

1) Disassemble the pipette as described in

"Maintenance".

2) Set aside the upper part in a dry and secure

location.

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Gilson PIPETMAN P Manuel utilisateur

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