Thermo Fisher Scientific CytoScan HT-CMA Assay 24-Array Format Le manuel du propriétaire

Marque: Thermo Fisher Scientific

Modèle: CytoScan HT-CMA Assay 24-Array Format

Taper: Le manuel du propriétaire

For Research Use Only. Not for use in diagnostic procedures.

CytoScan™ HT-CMA Assay 24-Array Format

Manual Workﬂow

SITE PREPARATION GUIDE

for use with:

CytoScan™ HT-CMA 24-Array Plate

CytoScan™ HT-CMA Reagent Kit 4x24F

Catalog Numbers 906018 and 906028

Publication Number MAN0018218

Revision A.0

Thermo Fisher Scientiﬁc Baltics

UAB |

V.A. Graiciuno 8, LT-02241 |

Vilnius, Lithuania

Products:

CytoScan™ HT-CMA Reagent Kit 4x24F

Affymetrix Pte Ltd |

7 Gul Circle #2M-01 |

Keppel Logistics Building |

Singapore 629563

Products:

CytoScan™ HT-CMA 24-Array Plate

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL,

INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,

INCLUDING YOUR USE OF IT.

Revision history: Pub. No. MAN0018218

Revision Date Description

A.0 06 February 2020 New document.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept

the terms and conditions of all applicable Limited Use Label Licenses.

TRADEMARKS: All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed. Allegra 25R and DTX 880

are registered trademarks of Beckman Coulter, Inc. BINDER ED 56 is a trademark of BINDER GmbH. Bio-Rad HardShell and Microseal are registered

trademarks of Bio-Rad Laboratories. CoolSafe is registered trademark of Diversiﬁed Biotech. Mastercycler pro S is a registered trademark, and

Deepwell Plate 96 is a trademark, of Eppendorf AG. Greiner Bio-One is a trademark of Greiner Bio One International GmbH. Kimwipes is a registered

trademark of Kimberly-Clark, Inc. Pipet-Aid is a trademark of Drummond Scientiﬁc Company, Inc. Pipet-Lite and Green-Pak are trademarks of

Mettler-Toledo Rainin, LLC. Scienceware Cryo-Safe and Bel-Art are registered trademarks of Scienceware, Inc. SpectraMax is a registered trademark

of Molecular Devices. The Jitterbug is a trademark of Boekel Scientiﬁc. Vortex-Genie 2 is a registered trademark of Scientiﬁc Industries, Inc.

Contents

■CHAPTER 1 Equipment and supplies required ........................ 4

Equipment, software, reagents, and arrays required ................................. 4

Equipment required for manual target preparation .................................. 6

Preampliﬁcation and ampliﬁcation staging areas ............................... 6

Oven requirements .......................................................... 7

Spectrophotome-ters ....................................................... 7

Thermal cycler recommendations and protocol ................................. 8

Thermal cycler consumables ................................................. 8

Plate centrifuges ........................................................... 9

Sample plates ............................................................. 10

Pipettes and tips ........................................................... 10

Other labware required ..................................................... 11

Other equipment, reagents, and supplies required ............................. 12

■APPENDIX A Safety ..................................................... 14

Chemical safety ................................................................ 14

Biological hazard safety ......................................................... 16

■Documentation and support ............................................. 17

Related documentation ......................................................... 17

Customer and technical support ................................................. 18

Limited product warranty ....................................................... 18

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Equipment and supplies required

This site preparation guide includes the supplier and part number information for the

equipment, software, reagents, arrays, labware and other consumables that have been

veried for use with the CytoScan™ HT-CMA Assay 24-Array Format Manual

Workow.

Equipment, software, reagents, and arrays required

Unless otherwise indicated, all materials are available through thermosher.com.

MLS: Fisher Scientic (sherscientic.com) or other major laboratory supplier.

Table 1 Equipment, software, reagents, and arrays required

✓Item Source

Equipment

GeneTitan™ Multi-Channel (MC) Instrument[1] Contact Thermo Fisher Scientiﬁc

Software

GeneChip™ Command Console™ (GCC), version 4.3 or later Supplied with the GeneTitan™

System

Reproductive Health Research Analysis Software (RHAS), version 1.0 Download from

apps.thermoﬁsher.com

Multi Sample Viewer Software, version 1.1 Included with RHAS

Chromosome Analysis Suite (ChAS), version 4.1 Download from

apps.thermoﬁsher.com

Array plate

CytoScan™ HT-CMA 24-Array Plate Cat. No. 906018

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

✓Item Source

Reagents

CytoScan™ HT-CMA Reagent Kit 4x24F

Kit contains the following modules.

• HT Target Prep Module 1 (quantity 1, Part No. 906011)

• HT Target Prep Module 2‑1 (quantity 1, Part No. 906012)

• HT Target Prep Module 2‑2 (quantity 1, Part No. 906013)

• HT Target Prep Module 3‑1 (quantity 1, Part No. 906014)

• HT Target Prep Module 3‑2 (quantity 1, Part No. 906015)

• HT Target Prep Wash A (quantity 8, Part No. 906022)

• HT Target Prep Wash B (quantity 4, Part No. 906023)

• HT Target Prep Water (quantity 4, Part No. 906020)

• HT Target Prep Module 4‑1 (quantity 1, Part No. 906016)

• HT Target Prep Module 4‑2 (quantity 1, Part No. 906017)

Cat. No. 906028

Genomic DNA Standard (Ref 103) 951957

Other reagent or consumables kits

Axiom™ GeneTitan™ Consumables Kit

Kit contains the following items.

• Hybridization tray (quantity 1)

• Scan tray with top cover and protective base (quantity 1)

• Stain trays with top covers (quantity 5)

These trays are required for processing CytoScan™ HT-CMA Array

Plates on the GeneTitan™ Multi-Channel Instrument[1].

Cat. No. 901606

CytoScan™ HT-CMA Assay Kit 4x24F

Kit contains the following items.

• CytoScan™ HT-CMA Reagent Kit 4x24F (quantity 1, Cat. No.

906028)

• CytoScan™ HT-CMA 24-Array Plate (quantity 4, Cat. No. 906018)

• Axiom™ GeneTitan™ Consumables Kit (quantity 4, Cat. No. 901606)

Cat. No. 906029

CytoScan™ HT-CMA Training Kit 4x24F

Kit contains the following items.

• CytoScan™ HT-CMA Reagent Kit 4x24F (quantity 1, Cat. No.

906028)

• CytoScan™ HT-CMA 24-Array Plate (quantity 4, Cat. No. 906018)

• Axiom™ GeneTitan™ Consumables Kit (quantity 4, Cat. No. 901606)

• CarrierScan™ Training Plate (quantity 1, Part No. 931938)

Cat. No. 906031

[1] For a complete list of equipment and supplies required to install and operate the GeneTitan™ Instrument, see the

GeneTitan

™

Multi-Channel

Instrument Site Preparation Guide

(Pub. No. 08-0305).

Chapter 1 Equipment and supplies required

Equipment, software, reagents, and arrays required

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Equipment required for manual target preparation

Table 2 Additional instruments required for the CytoScan™ HT-CMA Assay 24-Array Format Manual Workﬂow

✓Equipment Source

Two ovens required.

See Table 3.

Fume hood

Strongly recommended. See the

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow User Guide

(Pub. No. MAN0018217).

One of the following thermal cyclers:

• Applied Biosystems™ GeneAmp™ PCR System 9700 (with

gold-plated or silver block)

• Applied Biosystems™ Veriti™ Thermal Cycler

• Applied Biosystems™ ProFlex™ PCR System

• Eppendorf™ Mastercycler™ pro S

See “Thermal cycler recommendations and protocol“ on

page 8.

Thermo Fisher Scientiﬁc

Eppendorf™

One of the following plate shakers:

• Compact Digital Microplate Shaker

• The Jitterbug™

Thermo Fisher Scientiﬁc

Cat. No. 88880023

Boekel Scientiﬁc™

Cat. No. 130000

When manipulating genomic DNA or seing up amplication reactions, take

precautions to avoid contamination with foreign DNA amplied in other reactions

and procedures. We recommend that genomic DNA manipulations and amplication

reaction setup be performed in dedicated rooms or areas separate from the main

laboratory.

Each area should have dedicated sets of pipees and plasticware. If no dedicated area

is available, use a dedicated bench or a dedicated biosafety hood and provide

dedicated pipees. If no dedicated bench or biosafety hood is available, a set of

dedicated pipees is recommended.

Ideally, the preamplication and amplication staging areas would be separate.

However, these areas may be combined due to space and equipment limitations.

Preampliﬁcation

and ampliﬁcation

staging areas

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Two ovens are required for the CytoScan™ Assay Manual Workow target

preparation using the standard overnight DNA precipitation workow.

Table 3 Recommended ovens for the CytoScan™ HT-CMA Assay 24-Array Format

Manual Workﬂow

✓Item Source

Two ovens, any combination of the following.

BINDER™ ED 56 Drying and Heating

Chamber[1]

BINDER™

ED056UL

(120 V, ~60 Hz)

Cat. No. 9010-0334

ED056

(230 V, 50/60 Hz)

Cat. No. 9010-0333

GeneChip™ Hybridzation Oven 645[2] Thermo Fisher Scientiﬁc

Cat. No. 00-0331

[1] Replaces BINDER™ Model ED 53.

[2] The GeneChip™ Hybridzation Oven 640 is currently not supported with the CytoScan™ HT-CMA Assay 24-Array

Format Manual Workflow.

We recommend using one of the spectrophotometers listed in Table 4.

Table 4 Recommended spectrophotometers for the CytoScan™ HT-CMA Assay 24-

Array Format Manual Workﬂow

✓Item Source

DTX 880 Multimode Detector Beckman Coulter™

Cat. No. 987921

Genomic Filter Slide Beckman Coulter™

Cat. No. A30184

SpectraMax® Plus 384 Microplate Reader Molecular Devices®

Cat. No. PLUS 384

Oven

requirements

Spectrophotome-

ters

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

The thermal cyclers in Table 5 have been veried for the CytoScan™ HT-CMA Assay

24-Array Format Manual Workow. Use of other thermal cyclers for this stage may

result in assay failure and may violate the array and reagent replacement policy.

Table 5 Recommended thermal cyclers for the CytoScan™ HT-CMA Assay 24-Array

Format Manual Workﬂow

✓Item Source

Applied Biosystems™ GeneAmp™ PCR System 9700

(with gold-plated or silver block)

Thermo Fisher Scientiﬁc

Applied Biosystems™ Veriti™ Thermal Cycler Thermo Fisher Scientiﬁc

Applied Biosystems™ ProFlex™ PCR System Thermo Fisher Scientiﬁc

Eppendorf™ Mastercycler™ pro S Eppendorf™

95°C

48°C 48°C

10 minutes

3 minutes Infinity

Figure 1 CytoScan HT-CMA Denature thermal cycler protocol (stage 4)

WARNING! Evaporation during denaturation can negatively impact assay

performance. Use the recommended thermal cycler consumables and sealing

lm to eliminate condensation and evaporation.

Table 6 provides details on the consumables required for the recommended thermal

cyclers.

Table 6 Thermal cycler consumables for speciﬁc thermal cycler models

Thermal cycler Plates Seals

Applied Biosystems™ GeneAmp™ PCR

System 9700 Hard-Shell™ 96-Well PCR Plate, high

proﬁle, semi skirted

Bio-Rad Laboratories™

Cat. No. HSS9641 MicroAmp™ Clear Adhesive Film

Applied Biosystems™

Cat. No. 4306311[1]

Applied Biosystems™ Veriti™ Thermal

Cycler

Applied Biosystems™ ProFlex™ PCR

System

Eppendorf™ Mastercycler™ pro S

Hard-Shell™ 96-Well PCR Plate, low

proﬁle, full skirted

Bio-Rad Laboratories™

Cat. No. HSP9631

[1] Microseal™ 'B' PCR Plate Sealing Film (Bio-Rad™, Cat. No. MSB1001) can be used instead of MicroAmp™ Clear Adhesive Film for Applied

Biosystems™ thermal cyclers.

Thermal cycler

recommendations

and protocol

Thermal cycler

consumables

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

One plate centrifuge is required for the CytoScan™ HT-CMA Assay 24-Array Format

Manual Workow. We recommend the plate centrifuges listed in Table 7. When

centrifuging and drying pellets, the centrifuge must be able to spin down plates at the

conditions shown below.

• rcf: 3200 x g with an appropriate rotor-bucket combination, or 4,000 rpm for the

Eppendorf™ Centrifuge 5810 R conguration described below.

• Temperature: 4°C

Relative centrifugal force (rcf) can be calculated as follows:

rcf = (1.118 × 10-5) R S2

where R is the radius of the rotor in centimeters and S is the speed of the centrifuge in

revolutions per minute.

In addition, the boom of the rotor buckets should be soft rubber to ensure that the

deep well plates do not crack. Do not use buckets that sit plates directly on a metal or

hard plastic boom. For the Eppendorf™ Centrifuge 5810 R, do not use the A-4-62

rotor with a WO-15 hard boom plate carrier.

Table 7 Recommended plate centrifuges for the CytoScan™ HT-CMA Assay 24-Array Format Manual Workﬂow

✓Item Source

Allegra™ 25R Benchtop Centrifuge, Refrigerated Beckman Coulter™

60 Hz, 280 V: Cat. No. 369434

50 Hz, 230 V: Cat. No. 369436

50/60 Hz, 200 V: Cat. No. 369435

S5700 Swinging-Bucket Rotor Beckman Coulter™ Cat. No. 368954

Centrifuge 5810 R Eppendorf™

120 V, 60 Hz, 15 Amp: Cat. No. 022625501

120 V, 50 Hz, 20 Amp: Cat. No. 022625101

230 V, 50 Hz: Cat. No. 022625551

Rotor A-4-81, with 4 MTP/Flex buckets Eppendorf™ Cat. No. 022638807

Sorvall™ Legend XTR Centrifuge (refrigerated) Thermo Scientiﬁc™

120 V, 60 Hz: Cat. No. 75004521

230 V, 50/60 Hz: Cat. No. 75004523 (USA and Canada)

230 V, 50 Hz: Cat. No. 75004520

TX-750 4 + 750mL Swinging Bucket Rotor Thermo Scientiﬁc™ Cat. No. 75003180

Microplate/Flask Carriers Thermo Scientiﬁc™

Set of 2 carriers: Cat. No. 75003795

Set of 4 carriers: Cat. No. 75003617

Plate centrifuges

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Table 8 Sample plates required for the CytoScan™ HT-CMA Assay 24-Array Format

Manual Workﬂow

✓Item Source

Hard-Shell™ 96-Well PCR Plate, high proﬁle, semi

skirted

Use with:

• Applied Biosystems™ GeneAmp™ PCR System

9700 (with gold-plated or silver block)

• Applied Biosystems™ Veriti™ Thermal Cycler

• Applied Biosystems™ ProFlex™ PCR System

Bio-Rad Laboratories™

Cat. No. HSS9641

Hard-Shell™ 96-Well PCR Plate, low proﬁle, full

skirted

Use with:

• Eppendorf™ Mastercycler™ pro S

Bio-Rad Laboratories™

Cat. No. HSP9631

Eppendorf™ Deepwell Plate 96 Fisher Scientiﬁc™

Cat. No. 13-864-302

Greiner Bio-One™ UV-Star™ 96-Well UV Spectroscopy

Microplate

Fisher Scientiﬁc™

Cat. No. 07-000-407

Table 9 Recommended Rainin™ pipettes and tips for the CytoScan™ HT-CMA Assay

24-Array Format Manual Workﬂow

✓Item Source

Pipet-Lite™ Magnetic Assist Pipet,

single-channel P20

Rainin™ Cat. No. L-20[1]

Pipet-Lite™ Magnetic Assist Pipet,

single-channel P200

Rainin™ Cat. No. L-200[1]

Pipet-Lite™ Magnetic Assist Pipet,

single-channel P1000

Rainin™ Cat. No. L-1000[1]

Pipette 12-channel P20 Rainin™ Cat. No. L12-20[1]

Pipette 12-channel P50 (

optional

) Rainin™ Cat. No. L12-50[1]

Pipette 12-channel P200 Rainin™ Cat. No. L12-200[1]

Pipette 12-channel P1200 Rainin™ Cat. No. L12-1200[1]

Pipette 8-channel P20 Rainin™ Cat. No. L8-20[1]

Pipette 8-channel P200 Rainin™ Cat. No. L8-200[1]

Pipette tips Green-Pak™ 10-µL reﬁll Rainin™ Cat. No. 30389274

Pipette tips Green-Pak™ 200-µL reﬁll Rainin™ Cat. No. 30389276

Pipette tips Green-Pak™ 1,000-µL reﬁll Rainin™ Cat. No. 30389272

Sample plates

Pipettes and tips

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

✓Item Source

Pipette tips RT 10 µL (racked tips) Rainin™ Cat. No. 30389225

Pipette tips RT 200 µL (racked tips) Rainin™ Cat. No. 30389239

Pipette tips RT 1,000 µL (racked tips) Rainin™ Cat. No. 30389212

[1] Equivalent item is acceptable.

Unless otherwise indicated, all materials are available through thermosher.com.

MLS: Fisher Scientic (sherscientic.com) or other major laboratory supplier.

Table 10 Additional labware required for the CytoScan™ HT-CMA Assay 24-Array

Format Manual Workﬂow

✓Item Source

Microcentrifuge tubes and tube holder MLS

15-mL sterile polypropylene conical centrifuge tube MLS

15-mL tube holder MLS

Matrix™25-mL Sterile Reagent Reservoir with Divider Thermo Fisher Scientiﬁc

Cat. No. 8095

CoolSafe™ Cooling Chamber for 0.2ml tubes Diversiﬁed Biotech™

Cat. No. CHAM-1000

VWR™ Disposable 10-mL Serological Pipet VWR™

Cat. No. 89130-898

VWR™ Disposable 5-mL Serological Pipet VWR™

Cat. No. 89130-896

One of the following sealing ﬁlms:

MicroAmp™ Clear Adhesive Film

Microseal™ 'B' PCR Plate Sealing Film

Thermo Fisher Scientiﬁc

Cat. No. 4306311

Bio-Rad Laboratories™

Cat. No. MSB1001

Kimwipes™ Delicate Task Wipes Fisher Scientiﬁc™

Cat. No. 06-666A

Other labware

required

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Unless otherwise indicated, all materials are available through thermosher.com.

MLS: Fisher Scientic (sherscientic.com) or other major laboratory supplier.

Table 11 Additional materials required for the CytoScan™ HT-CMA Assay 24-Array Format Manual Workﬂow

✓Item Source

Common laboratory equipment

Freezer, −20°C MLS

Refrigerator, 2–8°C MLS

Ice bucket, 4–9 L MLS

Fine-point permanent markers MLS

Vortex-Genie 2 Fisher Scientiﬁc™

120 V, 60 Hz: Cat. No. 50-728-002

230 V, 50 Hz: Cat. No. 50-728-004

Mini Microcentrifuge for 2-mL tubes MLS

Bel-Art™ Cryo-Safe™ Mini Cooler[1] Fisher Scientiﬁc™

Cat. No. 03-410-497

Pipet-Aid™ Pipette Controller[1] VWR™

Cat. No. 53106-220

GeneTitan™ ZeroStat AntiStatic Gun Thermo Fisher Scientiﬁc

Cat. No. 74-0014

Reagents

Quant-iT™ PicoGreen™ dsDNA Assay Kit Thermo Fisher Scientiﬁc

Cat. No. P7589

Reduced EDTA TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM

EDTA)

Fisher Scientiﬁc™

Cat. No. AAJ75793AE

2-Propanol, anhydrous, 99.5% (Isopropanol) Sigma-Aldrich™

Cat. No. 278475

Reagents, gels, and instruments required to run quality-control steps

Mother E-Base™ Device Thermo Fisher Scientiﬁc

Cat. No. EBM03

Daughter E-Base™ Device Thermo Fisher Scientiﬁc

Cat. No. EBD03

E‑Gel™

Agarose Gels, 4% Thermo Fisher Scientiﬁc

Cat. No. G800804

E‑Gel™

Agarose Gel, 1% Thermo Fisher Scientiﬁc

Cat. No. G800801

E‑Gel™ 96 High Range DNA Marker Thermo Fisher Scientiﬁc

Cat. No. 12352019

Other equipment,

reagents, and

supplies required

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

✓Item Source

TrackIt™ Cyan/Orange Loading Buffer Thermo Fisher Scientiﬁc

Cat. No. 10482028

25 bp DNA Ladder, or a similar product prepared as

instructed by the manufacturer.

Thermo Fisher Scientiﬁc

Cat. No. 931343

Water, Nuclease-free, Molecular Biology Grade, Ultrapure MLS

[1] Equivalent products from other manufacturers are acceptable.

Chapter 1 Equipment and supplies required

Equipment required for manual target preparation

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Safety

Chemical safety

WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards,

ensure laboratory personnel read and practice the general safety guidelines for

chemical usage, storage, and waste provided below. Consult the relevant SDS

for specic precautions and instructions:

·Read and understand the Safety Data Sheets (SDSs) provided by the

chemical manufacturer before you store, handle, or work with any chemicals

or hazardous materials. To obtain SDSs, see the "Documentation and

Support" section in this document.

·Minimize contact with chemicals. Wear appropriate personal protective

equipment when handling chemicals (for example, safety glasses, gloves, or

protective clothing).

·Minimize the inhalation of chemicals. Do not leave chemical containers open.

Use only with sucient ventilation (for example, fume hood).

·Check regularly for chemical leaks or spills. If a leak or spill occurs, follow

the manufacturer cleanup procedures as recommended in the SDS.

·Handle chemical wastes in a fume hood.

·Ensure use of primary and secondary waste containers. (A primary waste

container holds the immediate waste. A secondary container contains spills

or leaks from the primary container. Both containers must be compatible

with the waste material and meet federal, state, and local requirements for

container storage.)

·After emptying a waste container, seal it with the cap provided.

·Characterize (by analysis if needed) the waste generated by the particular

applications, reagents, and substrates used in your laboratory.

·Ensure that the waste is stored, transferred, transported, and disposed of

according to all local, state/provincial, and/or national regulations.

·IMPORTANT! Radioactive or biohazardous materials may require special

handling, and disposal limitations may apply.

AVERTISSEMENT ! PRÉCAUTIONS GÉNÉRALES EN CAS DE

MANIPULATION DE PRODUITS CHIMIQUES. Pour minimiser les risques,

veiller à ce que le personnel du laboratoire lise aentivement et mee en œuvre

les consignes de sécurité générales relatives à l’utilisation et au stockage des

produits chimiques et à la gestion des déchets qui en découlent, décrites ci-

dessous. Consulter également la FDS appropriée pour connaître les précautions

et instructions particulières à respecter :

·Lire et comprendre les ches de données de sécurité (FDS) fournies par le

fabricant avant de stocker, de manipuler ou d’utiliser les matériaux

dangereux ou les produits chimiques. Pour obtenir les FDS, se reporter à la

section « Documentation et support » du présent document.

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

·Limiter les contacts avec les produits chimiques. Porter des équipements de

protection appropriés lors de la manipulation des produits chimiques (par

exemple : lunees de sûreté, gants ou vêtements de protection).

·Limiter l’inhalation des produits chimiques. Ne pas laisser les récipients de

produits chimiques ouverts. Ils ne doivent être utilisés qu’avec une

ventilation adéquate (par exemple, sorbonne).

·Vérier régulièrement l’absence de fuite ou d’écoulement des produits

chimiques. En cas de fuite ou d’écoulement d’un produit, respecter les

directives de neoyage du fabricant recommandées dans la FDS.

·Manipuler les déchets chimiques dans une sorbonne.

·Veiller à utiliser des récipients à déchets primaire et secondaire. (Le récipient

primaire contient les déchets immédiats, le récipient secondaire contient les

fuites et les écoulements du récipient primaire. Les deux récipients doivent

être compatibles avec les matériaux mis au rebut et conformes aux exigences

locales, nationales et communautaires en matière de connement des

récipients.)

·Une fois le récipient à déchets vidé, il doit être refermé hermétiquement avec

le couvercle fourni.

·Caractériser (par une analyse si nécessaire) les déchets générés par les

applications, les réactifs et les substrats particuliers utilisés dans le

laboratoire.

·Vérier que les déchets sont convenablement stockés, transférés, transportés

et éliminés en respectant toutes les réglementations locales, nationales et/ou

communautaires en vigueur.

·IMPORTANT ! Les matériaux représentant un danger biologique ou

radioactif exigent parfois une manipulation spéciale, et des limitations

peuvent s’appliquer à leur élimination.

WARNING! HAZARDOUS WASTE (from instruments). Waste produced by

the instrument is potentially hazardous. Follow the guidelines noted in the

preceding General Chemical Handling warning.

WARNING! 4L Reagent and Waste Bole Safety. Four-liter reagent and waste

boles can crack and leak. Each 4-liter bole should be secured in a low-density

polyethylene safety container with the cover fastened and the handles locked in

the upright position.

Appendix A Safety

Chemical safety

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Biological hazard safety

WARNING! Potential Biohazard. Depending on the samples used on this

instrument, the surface may be considered a biohazard. Use appropriate

decontamination methods when working with biohazards.

WARNING! BIOHAZARD. Biological samples such as tissues, body uids,

infectious agents, and blood of humans and other animals have the potential to

transmit infectious diseases. Conduct all work in properly equipped facilities

with the appropriate safety equipment (for example, physical containment

devices). Safety equipment can also include items for personal protection, such

as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety

glasses, or goggles. Individuals should be trained according to applicable

regulatory and company/ institution requirements before working with

potentially biohazardous materials. Follow all applicable local, state/provincial,

and/or national regulations. The following references provide general

guidelines when handling biological samples in laboratory environment.

·U.S. Department of Health and Human Services, Biosafety in Microbiological

and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC)

21-1112, Revised December 2009; found at:

hps://www.cdc.gov/labs/pdf/

CDC-BiosafetymicrobiologicalBiomedicalLaboratories-2009-P.pdf

·World Health Organization, Laboratory Biosafety Manual, 3rd Edition,

WHO/CDS/CSR/LYO/2004.11; found at:

www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf

Appendix A Safety

Biological hazard safety

CytoScan

™

HT-CMA Assay 24-Array Format Manual Workﬂow Site Preparation Guide

Documentation and support

Thermo Fisher Scientific CytoScan HT-CMA Assay 24-Array Format Le manuel du propriétaire

Marque: Thermo Fisher Scientific

Modèle: CytoScan HT-CMA Assay 24-Array Format

Taper: Le manuel du propriétaire

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English: Thermo Fisher Scientific CytoScan HT-CMA Assay 24-Array Format Owner's manual

Thermo Fisher Scientific CytoScan HT-CMA Assay 24-Array Format Le manuel du propriétaire

Thermo Fisher Scientific CytoScan HT-CMA Assay 24-Array Format Le manuel du propriétaire

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