Thermo Fisher Scientific Dionex UltiMate 3000 Series Mode d'emploi

Marque: Thermo Fisher Scientific

Modèle: Dionex UltiMate 3000 Series

Taper: Mode d'emploi

Thermo Scientific Dionex

UltiMate 3000 Series

SR-3000 Solvent Rack

Operating Instructions

(Original Operating Instructions)

Revision: 1.7

Date: September, 2013

Doc. No. 4820.3555

*4820.3555*

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page I

Declaration of Conformity

(Original Declaration of Conformity)

Product: Thermo Scientific Dionex UltiMate 3000 Solvent Rack

Types: SR-3000, SRD-3200, SRD-3400, SRD-3600

Dionex Softron GmbH herewith declares conformity of the above products with the

respective requirements of the following regulations:

• Low-Voltage Directive 2006/95/EC

• EMC Directive 2004/108/EC

The electrical safety of the products was evaluated based on the following standard:

• DIN EN 61010-1:2010

Safety requirements for electrical equipment for measurement, control and

laboratory use, Part 1: General Requirements

The Electromagnetic Compatibility (EMC) of the products was evaluated based on the

following standard:

• DIN EN 61326:2006

Electrical equipment for measurement, control and laboratory use

EMC Requirements

This declaration is issued for the manufacturer

Dionex Softron GmbH

Part of Thermo Fisher Scientific Inc.

Dornierstraße 4

D-82110 Germering

by the Managing Director, Rüdiger Obst and

the Vice President HPLC, Fraser McLeod.

September 2, 2013

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page II Operating Instructions

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page i

Table of Contents

1 Introduction ................................................................................................................... 1

1.1 How to Use This Manual ........................................................................................... 1

1.2 Safety Information ..................................................................................................... 2

1.2.1 Symbols on the Instrument and in the Manual .................................................. 2

1.2.2 Safety Precautions .............................................................................................. 3

1.2.3 Consignes de Sécurité ........................................................................................ 6

1.3 Intended Use .............................................................................................................. 9

1.4 Federal Communications Commission (FCC) Note .................................................. 9

2 Installation and Maintenance .................................................................................... 11

2.1 General Information ................................................................................................. 11

2.2 Unpacking ................................................................................................................ 11

2.3 Positioning the Solvent Rack in the UltiMate 3000 System .................................... 12

2.4 Inside View .............................................................................................................. 13

2.5 Solvent Reservoirs ................................................................................................... 14

2.5.1 General Precautions ......................................................................................... 14

2.5.2 Connecting the Solvent Reservoirs .................................................................. 15

2.6 Routine and Preventive Maintenance ...................................................................... 16

3 Technical Information ................................................................................................ 17

4 Accessories, Consumables, and Spare Parts ............................................................ 19

4.1 Standard Accessories ............................................................................................... 19

4.2 Consumables and Spare Parts .................................................................................. 19

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page ii Operating Instructions

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 1

1 Introduction

1.1 How to Use This Manual

The layout of this manual is designed to provide quick reference to the sections of interest

to the reader. However, in order to obtain a full understanding of the Thermo Scientific

Dionex™ UltiMate™ 3000 Solvent Rack, Thermo Fisher Scientific recommends that you

review the manual thoroughly before beginning operation.

This manual is provided "as is." Every effort has been made to supply complete and

accurate information and all technical specifications have been developed with the utmost

care. The information contained in this manual should not be construed as a commitment

by Thermo Fisher Scientific. Thermo Fisher Scientific assumes no responsibility for any

errors that may appear in this document. This document is believed to be complete and

accurate at the time of publication. In no event shall Thermo Fisher Scientific be liable for

incidental or consequential damages in connection with or arising from the use of this

document. The information contained in this document is subject to change without notice.

electronic media. No part of this publication may be copied or distributed, transmitted,

transcribed, stored in a retrieval system, or transmitted into any human or computer language,

in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or

disclosed to third parties without the express written permission of Thermo Fisher Scientific

Inc.

Trademarks

PEEK is a trademark of Victrex PLC.

All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 2 Operating Instructions

1.2 Safety Information

The CE Mark label on the instrument indicates that the instrument is compliant with the

related standards.

1.2.1 Symbols on the Instrument and in the Manual

The table shows the symbols used on the instrument:

Symbol Description

WEEE (Waste Electrical and Electronic Equipment) label—For more

information, see the WEEE Information section in the "Installation and

Qualification Documents for Dionex Thermo Scientific Instruments" binder.

Étiquette DEEE (Déchets d'Equipements Electriques et Electroniques) — Pour

plus d'informations, référez-vous au chapitre WEEE Information dans le classeur

"Installation and Qualification Documents for Chromatography Instruments".

Refer to the operating instructions to prevent risk of harm to the operator and to

protect the instrument against damage.

Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou

protéger l'instrument contre tout dommage.

Label according to the "Measures for Administration of the Pollution Control of

Electronic Information Products" (China RoHS) guideline if necessary

Étiquette China RoHs si nécessaire

At various points throughout the manual, the following symbols indicate messages of

particular importance:

Tip: Indicates general information, as well as information intended to

optimize the performance of the instrument.

Important: Indicates that failure to take note of the accompanying information

could cause wrong results or may result in damage to the instrument.

Important: Indique que ne pas tenir compte de l'information jointe peut conduire

à de faux résultat ou endommager l'instrument.

Warning: Indicates that failure to take note of the accompanying information

may result in personal injury.

Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner

des blessures corporelles.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 3

1.2.2 Safety Precautions

When working with analytical instrumentation, you must know the potential hazards of

using chemical solvents.

Tip: Before initial operation, make sure that you are familiar with the

contents of this manual.

For the safety precautions in French, see section 1.2.3 (→ page 6).

Warning: All users of the device must observe the following safety precautions

and all additional safety precautions in this manual to avoid the

possibility of personal injury or damage to the device when operating

the device or carrying out any maintenance or service procedures.

Observe any warning labels on the device and see the related sections in

these Operating Instructions.

• Protective equipment

When performing any work on or near the HPLC system, wear personal protective

equipment (protective clothing, safety gloves, safety glasses) as required by the hazard of

the mobile phase and sample. For information about the proper handling of particular

substance and for advice on specific hazards, refer to the material safety data sheet for the

substance you are using. Observe the guidelines of Good Laboratory Practice (GLP).

An eyewash facility and a sink should be close to the device. If any substance splashes on

the eyes or skin, wash the affected area and seek medical attention.

• Hazardous substances

Many organic solvents, mobile phases, and samples are harmful to health. Be sure that

you know the toxic and infectious properties of all substances that you are using. You

may not know the toxic or infectious properties of many substances that you are using. If

you have any doubt about a substance, treat it as if it contains a potentially harmful

substance. For advice on the proper handling of a particular substance, refer to the Safety

Data Sheet (SDS) of the manufacturer. Observe the guidelines of Good Laboratory

Practice (GLP).

Dispose of waste substance in an environmentally safe manner that is consistent with all

local regulations. Do not allow flammable, toxic, and/or infectious substances to

accumulate. Follow a regulated, approved waste disposal program. Never dispose of

flammable, toxic, and/or infectious substances through the municipal sewage system.

• Hazardous gases

Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample

includes volatile or flammable solvents, do not allow them to enter the workspace. If the

mobile phase or sample includes volatile or flammable solvents, avoid open flames and

sparks.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 4 Operating Instructions

• Electrostatic discharge

Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.

Keep in mind that liquid flowing through capillaries can generate static electricity. This

effect is particularly pronounced in insulating capillaries and with non-conductive

solvents (for example, pure acetonitrile).

Take appropriate measures to prevent the generation of static electricity near the HPLC

system. For example, make sure that the air humidity level in the laboratory is

sufficiently high and provide proper ventilation, wear anti-static clothing or shoes,

prevent accumulation of air bubbles in waste lines, and use grounded waste containers.

Use only non-conductive capillaries to direct solvents into the waste container. With

electrically conductive capillaries, make sure that they are properly grounded.

• Self-ignition of solvents

Do not use solvents for which the self-ignition temperature is below 150 °C. In case of

leakage, these solvents may self-ignite on a hot surface.

• Capillaries, capillary connections, open connections

♦ Capillaries, especially non-metallic capillaries may burst, slip out of their fittings or

may not be screwed in. This may result in substances spraying out of the open

connections.

♦ In an UltiMate 3000 system, some components are made of PEEK™. This polymer

has superb chemical resistance to most organic solvents. However, it tends to swell

when in contact with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or

tetrahydrofuran (THF). In addition, it is attacked by concentrated acids, such as,

sulfuric acid and nitric acid or a mixture of hexane, ethyl acetate, and methanol. In

both cases, capillaries may start leaking or they can burst. Swelling or attack by

concentrated acids is not a problem with brief flushing procedures.

♦ Do not use tubing that is stressed, bent, kinked, or damaged.

♦ Capillary connections can be contaminated by harmful substances or harmful

substances can escape from open connections.

♦ Some capillaries of the RS pumps and some Viper system capillaries are made of

MP35N®, a nickel-cobalt based alloy. Individuals with sensitivity to nickel/cobalt may

show an allergic reaction from skin contact.

♦ Always wear safety glasses when handling fused silica tubing, for example, during

installation or when cutting capillaries to the length.

• For safety reasons, you shall not lift the SRD with one or more solvent reservoirs being in

the tray.

• Use only the original spare parts and accessories authorized for the device by Thermo

Fisher Scientific.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 5

• When operating the HPLC system, always set a lower pressure limit for the pump. This

prevents damage resulting from leakage or from running the pump dry over a longer

period. Activate solvent reservoir level monitoring (→ Pump manual).

• The front panel tilts upward. When lifting or moving the SRD, always lift by the bottom

or sides of the instrument. This is to avoid damage to the instrument.

• The open front panel door is not designed to carry weight. Therefore, you shall not place

any objects on the open front panel door.

• Do not use the solvent rack in ways other than those described in this manual.

• Keep the operating instructions near the device to be available for quick reference.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 6 Operating Instructions

1.2.3 Consignes de Sécurité

Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation

de produit chimiques.

Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous

vous êtes familiarisés avec le contenu de ce manuel.

Avertissement: Toutes les personnes utilisant l’instrument doivent observer les

consignes de sécurité suivantes et dans les autres chapitres de ce

manuel pour éviter une mise en danger de sa personne ou de

dommage à l’instrument pendant l’utilisation et des opérations de

maintenance ou service de l’instrument.

Observez les étiquettes d'avertissement sur l'instrument et référez-

vous aux sections correspondantes dans ce mode d'emploi.

• Equipment de protection

Pour tous les travaux sur le système HPLC ou à proximité, portez l'équipment de

protection personnel (vêtements de protection, gant de sécurité, lunettes de protection)

qui correspond aux risque découlant de la phase mobile et/ou de l'échantillon. Pour les

informations sur la manipulation correcte des composés et des recommandations pour les

situations de risque spécifiques, veuillez consulter la fiche de données de sécurité des

substances que vous utilisez. Veuillez respecter des directives des Bonnes Pratiques de

Laboratoire (BPL).

Une installation permettant de se laver les yeux ainsi qu'un lavabo doivent se trouver à

proximité du système. Si une substance, quelle qu'elle soit, entre en contact avec vos

yeux ou votre peau, rincez abondamment la zone affectée à l’eau, puis.

• Substances dangereuses

De nombreux solvants organiques, phases mobiles et échantillons sont nuisibles à la

santé. Informez-vous de propriétés toxicologiques et infectieuses de toutes les substances

que vous utilisez. Les propriétés toxicologiques et infectieuses de nombreuses substances

peuvent être mal connues. Au moindre doute concernant une substance, traitez-la comme

s'il contenait une substance potentiellement dangereuse. Pour des instructions comment

utiliser correctement des composés particuliers, veuillez consulter à la fiche de données

des sécurités du fabricant respectif. Veuillez respecter des directives des Bonnes

Pratiques de Laboratoire (BPL).

Débarrassez-vous de tous les déchets de substances de manière écologique,

conformément à la règlementation en vigueur au niveau local. Empêchez impérativement

l'accumulation de solvants inflammables, toxiques et/ou infectieux. Suivez un

programme d'élimination des déchets règlementé et approuvé. Ne jetez jamais de

solvants inflammables, toxiques et/ou infectieux dans le système municipal d'évacuation

des eaux usées.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 7

• Gaz dangereux

Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou

l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne

pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des

solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à

proximité.

• Décharge électrostatique

Décharge électrostatique peut provoquer la formation d'étincelles et peut présenter un

risque d’incendie. Veuillez noter que des solvants fluides dans les capillaires peuvent se

charger automatiquement. Cet effet se peut produire particulièrement forte dans les

capillaires isolants et avec des solvants non-conducteurs (par exemple, l'acetonitrile pur).

Prenez des mesures appropriées pour éviter les charges électrostatiques à proximité du

système HPLC. Par exemple, s'assurez qu'il y a une humidité de l'air suffisante et une

ventilation adéquate dans la laboratoire, portez des vêtements ou équipement de

protection antistatique, évitez l'accumulation de bulles d'air dans les lignes de déchets et

utilisez des réservoirs à déchets mis à la terre.

Utilisez uniquement des capillaires non-conducteurs pour diriger solvants au réservoir de

déchets. Capillaires électriquement conducteur devrait être mis à la terre.

• Inflammation spontanée des solvants

N’utilisez aucun solvants avec une température d‘auto-inflammabilité inférieure à

150° C. Si une fuite se produit, ces solvants peuvent s’auto-enflammer au contact d’une

surface chaude.

• Capillaires, connecteur capillaires, connexions ouvertes

♦ Des capillaires, en particulier les capillaires non-métalliques, pourraient fendre ou

glisser des connecteurs ou ne peuvent pas être vissés. Ceci peut en résulter aussi que

des substances pourraient jaillir des connexions ouvertes.

♦ Dans un système UltiMate 3000, certaines composantes sont en PEEK. Bien que ce

polymère présente une excellente résistance chimique à la plupart des solvants

organiques, il a tendance à gonfler lorsqu'il est en contact prolongé avec du

chloroforme (CHCl3), du diméthyle sulfoxyde (DMSO) ou du tétrahydrofurane

(THF). De plus, il est attaqué par des acides concentrés tels que l'acide sulfurique et

l'acide nitrique ou d'un composé du hexane, éthyle acétate et méthanol. Ceci peut

causer des capillaires de fuite ou risquer des capillaires d’éclater. Ces acides peuvent

cependant être utilisés dans le cadre de procédures de nettoyage, à condition que

l’exposition soit brève.

♦ N'utilisez pas de capillaires écrasés, pliés, abimés ou endommagés.

♦ Les connecteurs capillaires pour pourrait être contaminé par des substances

dangereuses ou des substances dangereuses pourrait sortir des connexions ouvertes.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 8 Operating Instructions

♦ Certains capillaires des pompes RS, ainsi que des capillaires du système Viper, sont

faits d'alliage de nickel-cobalt MP35N. Contact avec la peau peut provoquer une

réaction chez les personnes qui sont sensibles au nickel/cobalt.

♦ Portez des lunettes de protection lorsque vous manipulez des capillaires en silice

fondue (pendant l'installation, découpe, etc.).

• Pour raison de sécurité, ne soulevez pas l'instrument avec des réservoirs.

• Utilisez seulement des pièces de rechange originales et des accessoires autorisés par

Thermo Fisher Scientific.

• Réglez toujours une limite de pression minimum pour le système HPLC. Ceci prévient

les dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe.

Activez la surveillance de niveau liquide pour des réservoirs (→ manuel de pompe).

• Lorsque vous soulevez ou l’instrument, tenez-le toujours par le dessous ou par les côtés

de l'unité. Soulever l’instrument par la partie avant inférieure ou par le panneau avant

peut endommager la porte.

• Ne placez aucun objet lourd sur la porte ouverte du panneau avant. Ceci pourrait

endommager la porte.

• N'utilisez pas l'instrument de manière autre que celles décrites dans ce manuel.

• Conservez ce manuel á proximité de l’instrument pour pouvoir le consulter facilement.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 9

1.3 Intended Use

For Research Use Only. Not for use in diagnostic procedures.

The device is designed to be operated only be qualified and authorized personnel. All users

must know the hazards presented by the device and the used substances.

1.4 Federal Communications Commission (FCC) Note

This equipment has been tested and found to comply with the limits for a Class A digital

device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide

reasonable protection against harmful interference when the equipment is operated in a

commercial environment. This equipment generates, uses, and can radiate radio frequency

energy and, if not installed and used in accordance with the instruction manual, may cause

harmful interference to radio communications. Operation of this equipment in a residential

area is likely to cause harmful interference, in which case the user will be required to

correct the interference at his expense.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 10 Operating Instructions

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 11

2 Installation and Maintenance

2.1 General Information

The solvent rack is a high-quality instrument designed for HPLC analysis as part of an

UltiMate 3000 system. The rack is intended for use as a solvent organizer, for example,

together with a LPG-3400 pump, providing a secure location for installation of the solvent

reservoirs and, when mounted on top of the UltiMate 3000 system, saves valuable bench

space.

All parts in the flow path are made of PEEK, FEP, stainless steel, or Titanium (biocompatible

system only) to provide optimum resistance to the most commonly used HPLC solvents and

buffer solutions.

2.2 Unpacking

All mechanical components of the solvent rack are carefully tested before the instrument is

shipped from the factory. After unpacking, inspect the rack for any signs of mechanical

damage that may have occurred during transit.

Tips: Immediately report any shipping damage to both, the incoming carrier and

Thermo Fisher Scientific. Shipping insurance will compensate for the damage

only if reported immediately.

Keep the original shipping container and the packing material. They will

provide excellent protection for the solvent rack in case of future transit.

Shipping the rack in any other packaging automatically voids the product

warranty. For more information, see the warranty statement in the terms of

sale.

1. Place the shipping container on the floor. Remove the accessories kit and solvent

bottles.

2. Grasp the solvent rack by the sides. Slowly and carefully, pull the rack out of the

shipping container and place it on a stable surface.

Important: To prevent the rack from falling, always lift the unit by the sides.

Do not lift the unit by the packaging material.

Important: Afin d'empêcher l'instrument de tomber, saisissez-la par les côtés.

Ne soulevez l'instrument à l’aide du matériau d'emballage.

3. Remove the foam spacers, and then remove the polythene packaging.

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 12 Operating Instructions

2.3 Positioning the Solvent Rack in the UltiMate 3000 System

If the solvent rack is part of an UltiMate 3000 system, for example, for analytical HPLC

applications, you should stack the individual modules, for example, as shown in Fig. 1.

However, the arrangement of the system modules depends on the application.

Fig. 1: Module arrangement for an UltiMate 3000 system (example)

If the solvent rack is part of an UltiMate 3000 RSLCnano or Proteomics MDLC system,

the related system manual provides information about how to arrange the system modules

and set up the systems for these applications.

Detector

Autosampler

Column Compartment

Pump

Solvent Rack

UltiMate 3000 Series:

SR-3000 Solvent Rack

Operating Instructions Page 13

2.4 Inside View

The front panel door tilts upward to provide access to the tubing guides in the solvent rack.

The open door locks in its topmost position.

Fig. 2: SR-3000 with open front panel

Warning: For safety reasons, you shall not lift the solvent rack when one or

more solvent reservoirs are in the tray.

Avertissement: Pour raison de sécurité, ne soulevez pas l'instrument avec des

réservoirs.

Important: When lifting or moving the solvent rack, always lift by the bottom or

sides of the unit. Lifting the solvent rack by the front panel may

damage the front panel door.

The open front panel door is not designed to carry weight. Therefore,

you should not place any objects on the open door.

Important: Lorsque vous soulevez ou l’instrument, tenez-le toujours par le

dessous ou par les côtés de l'unité. Soulever l’instrument par la partie

avant inférieure ou par le panneau avant peut endommager la porte.

Ne placez aucun objet sur la porte ouverte du panneau avant. Ceci

peut endommager la porte.

Tubing guides for the solvent lines

UltiMate 3000 Series:

SR-3000 Solvent Rack

Page 14 Operating Instructions

2.5 Solvent Reservoirs

The solvent rack is shipped with solvent reservoirs and appropriate tubing, including filter

holders and filter frits.

2.5.1 General Precautions

When connecting the solvent reservoirs, observe the following general precautions:

• Rinse the eluent reservoirs thoroughly before using them for the first time. Use high-

purity solvent for rising.

• Always install filter frits on the solvent lines. This prevents contaminants from reaching

the HPLC system.

• Regularly check the filter frits for permeability. Replace the filter frits at regular intervals.

This is especially important when using aqueous solvents. Aqueous solvents may

contaminate the filters with algae and other microorganisms that deposit on the filter frits.

Therefore, also replace the solvents at regular intervals. Rinse the reservoirs thoroughly

before refilling them. Replace the filter frits as necessary.

• As standard, the filter frits shipped with the solvent rack are stainless steel frits.

If the UltiMate 3000 system includes a biocompatible pump, NCS-3500RS, or

NCP-3200RS, replace the stainless frits with the frits from the accessories kit of the

module. To do so, unscrew the top part of the filter holder from its bottom part and

replace the filter frit. When placing the new filter frit into the bottom part, make sure that

the frit is in a level position (avoid tilting the frit).

If the UltiMate 3000 system includes a semipreparative pump, use the filter holder and

filter frit from the accessories kit of the pump.

• Locate the solvent reservoirs as close as possible to the pump. To avoid formation of gas

bubbles in the reservoirs, make sure that the reservoirs are on the same level or higher as

the pump. Therefore, stack the rack onto the pump as shown in Fig. 1 (→ page 12). The

tubing connecting the pump to the degasser should be as short as possible to avoid

reformation of gas bubbles in the solvent.

• When replacing solvents, make sure that the solvents are miscible. Mix immiscible

solvents with an intermediate solvent (for example, isopropanol) to replace them step-by-

step.

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