siRNA quality and amount .................................................. 24
Controls .................................................................. 24
Using the Neon™ Transfection System ............................................ 25
Materials needed .......................................................... 25
Set up the Neon™ pipette station ............................................ 26
Prepare adherent cells ...................................................... 27
Prepare suspension cells ................................................... 28
Electroporation protocol .................................................... 29
Optimization .............................................................. 33
Cleaning and maintenance .................................................. 33
Optimization protocol for DNA and siRNA ......................................... 33
Materials needed .......................................................... 33
General guidelines ......................................................... 34
24-well optimization protocol for adherent and suspension cell lines—day one .... 34
18-well optimization protocol for primary suspension blood cells—day one ....... 36
Optimization protocol—day two ............................................. 38
Optional: optimization protocol—day three .................................... 40
■APPENDIX A Troubleshooting .................................................... 42
Troubleshooting ................................................................ 42
Neon™ device error messages ................................................... 46
■APPENDIX B Maintenance ....................................................... 47
Repackaging the instrument ..................................................... 47
Repackaging and storage instructions ........................................ 47
Replace the Pipette Gripper ..................................................... 48
■APPENDIX C Specifications ...................................................... 51
Product specifications .......................................................... 51
■APPENDIX D Related products .................................................. 52
Accessory products ............................................................ 52
Additional products ........................................................ 52
Cell culture media ......................................................... 53
siRNA .................................................................... 53
■APPENDIX E Safety ............................................................... 54
Safety information .............................................................. 54
Informational symbols .......................................................... 54
Informations de sécurité ........................................................ 55
Informational symbols .......................................................... 56
Contents
4Neon™ Transfection System User Guide