Thermo Fisher Scientific SCOUT® Column Selector Mode d'emploi

SCOUT® Column Selector

User Guide

For Research Use Only. Not for use in diagnostic procedures.

Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that

may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall

Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use

of this document or the operation and use of the instrument.

Applied Biosystems, BioCAD, Perfusion Chromatography, POROS, SCOUT, VISION, and the fractal icon are

registered trademarks of Applied Biosystems or its subsidiaries in the U.S. and certain other countries.

AB (Design), ABI, Applera, ID ImmunoDetection, INTEGRAL, Poroszyme, and SPRINT are trademarks of Applied Biosystems

or its subsidiaries in the U.S. and certain other countries.

SLO-BLO is a trademark of Littlefuse, Inc.

Microsoft, MS, MS-DOS, and Windows are registered trademarks of Microsoft Corporation.

RheFlex is a registered trademark of Rheodyne, Inc.

All other trademarks are the sole property of their respective owners.

Printed in the USA, 04/2011

Part Number 8-0123-40-0795 Rev. B

Printed on recycled paper.

WARNING

Always wear eye protection when working with solvents or any pressurized liquids.

AVERTISSEMENT

Porter toujours des protections pour les yeux lorsque vous manipulez des solvants ou

tout autre liquide sous pression.

WARNING

Use this product only as specified in this document. Using this system in a manner not

specified may result in injury or damage to the system.

AVERTISSEMENT

Veuillez suivre avec attention les indications figurant dans ce document lorsque vous

utilisez ce produit. Un usage différent le système causer un accident ou endommager

le système.

WARNING

PEEK tubing is polymeric material. Use caution when working with any polymer tubing

under pressure.

1. Always wear eye protection when in close proximity to pressurized polymer tubing.

2. Extinguish all nearby flames if using flammable solvents.

3. Do not use PEEK tubing that has been severely stressed or kinked.

4. Do not use PEEK tubing with tetrahydrofuran or concentrated nitric and sulfuric

acids.

5. Methylene chloride and dimethyl sulfoxide cause PEEK tubing to swell and greatly

reduce the rupture pressure of the tubing.

6. With high solvent flow rates (>40 ml/min), a static charge may build up on the

surface of the tubing. Electrical sparks may result.

AVERTISSEMENT

Le tubage PEEK est un matériau polymère. Prenez des précautions lorsque vous

travaillez avec un tubage polymère sous pression.

1. Porter toujours des protections pour les yeux lorsque vous vous trouvez à

proximité d’un tubage polymère sous pression.

2. Éteindre toutes les flammes avoisinantes si vous utilisez des solvants

inflammables.

3. Nes pas utiliser un tubage PEEK qui a déjà été sérieusement travaillé ou plié.

4. Nes pas utiliser un tubage PEEK avec du tétrahydrofurane ou des acides nitriques

et sulfuriques concentrés.

5. Le chlorure de méthylène et le sulfoxyde de diméthyle entraînent un gonflement

du tubage PEEK et réduisent fortement la résistance à la rupture du tubage.

6. Avec des débits d’écoulement de solvants élevés (>á 40 ml/min), une charge

statique puet se développer sur la surface du tubage. Des étincelles électriques

peuvent en résulter.

WARNING

For continued protection against fire hazard, replace fuses with those of the same type

and rating.

AVERTISSEMENT

Pour une protection permanente contre le danger d’incendie, remplacez les fusibles

existants avec des fusibles similaires, présentant les mêmes caractéristiques.

WARNING

Before handling any chemicals, refer to the Material Safety Data Sheet provided by

the manufacturer, and observe all relevant precautions.

AVERTISSEMENT

Avant de manipuler des produits chimiques, veuillez consulter la fiche de sécurité du

matériel fournie par le fabricant, et observer les mesures de précaution qui

s'imposent.

CAUTION

Failure to flush the system after daily use may result in system downtime. Refer to

Section 3.6, Flushing the SCOUT Column Selector After Use, for the recommended

procedure.

US Safety and EMC (Electromagnetic Compatibility) Standards

Safety

This instrument has been tested to and complies with standard ANSI/UL 3101-1, “Electrical

Equipment for Laboratory Use; Part 1: General Requirements”, 1st Edition. It is an ETL Testing

Laboratories listed product.

EMC

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two

conditions: (1) This device may not cause harmful interference, and (2) this device must accept

any interference received, including interference that may cause undesired operation.

Warning: Changes or modifications to this unit not expressly approved by the party responsible for

compliance could void the user’s authority to operate the equipment.

Note: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against

harmful interference when the equipment is operated in a commercial environment. This equipment

generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with

the instruction manual, may cause harmful interference to radio communications. Operation of this

equipment in a residential area is likely to cause harmful interference in which case the user will be required

to correct the interference at his own expense.

Note: Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits.

Canadian Safety and EMC (Electromagnetic Compatibility) Standards

Safety

This instrument has been tested to and complies with standard C22.2 No. 151, “Safety

Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use; Part 1:

General Requirements”. It is an ETL Testing Laboratories listed product.

Sécurité

Cet instrument a été vérifié avec la norme C22.2 No. 151, «Spécifications de sécurité du matériel

électrique utilisé pour les mesures, les contrôles et dans les laboratoires ; Partie 1 : Spécifications

générales», et il est conforme à cette norme. C’est un produit homologué par les ETL Testing

Laboratories.

EMC

This Class A digital apparatus meets all requirements of the Canadian Interference-Causing

Equipment Regulations.

Cet appareil numérique de la classe A respecte toutes les exigences du Règlement sur le

materiel brouilleur du Canada.

European Safety and EMC (Electromagnetic Compatibility) Standards

Declaration of Conformity

Application of Council Directive(s): 73/23/EEC “Low Voltage”

89/336/EEC “Electromagnetic Compatibility”

Standard(s) to which conformity is

declared: EN61010-1 “Safety Requirements for Electrical Equipment for Mea-

surement, Control and Laboratory Use”

EN55011:1991, Group 1, Class B “Radiated Emissions”

EN50082-1:1991 “Generic Immunity”

Manufacturer’s Name: Applied Biosystems

Manufacturer’s Address: 500 Old Connecticut Path

Framingham, Massachusetts 01701 USA

Type of Equipment: Laboratory Instrumentation

Model Name & Number: SCOUT Column Selector

Part Number: 5-2287-00

Serial Number: 250 and later

Year of Manufacture: 1997

Table of Contents

SCOUT

®

Column Selector User Guide ix

Table of Contents

How to Use This Guide........................................................................... xi

Chapter 1 Introducing the SCOUT Column Selector

1.1 SCOUT Column Selector Features....................................................... 1-2

1.2 Parts of the SCOUT Column Selector................................................... 1-3

Chapter 2 Installing a Stand-Alone SCOUT Column

Selector

2.1 Installing the Hardware......................................................................... 2-2

2.2 Installing the Software.......................................................................... 2-5

Chapter 3 Using the SCOUT Column Selector

3.1 Plumbing the SCOUT Column Selector ................................................ 3-2

3.1.1 Preparing SCOUT Hex Nut Fittings ...................................... 3-2

3.1.2 Connecting Columns to the SCOUT Column Selector .......... 3-4

3.1.3 Plumbing the SCOUT Column Selector to Your System ....... 3-6

3.2 Configuring the SCOUT Columns......................................................... 3-7

3.3 Running a SCOUT Method................................................................... 3-9

3.3.1 Editing General Settings ...................................................... 3-9

3.3.2 Adding a SCOUT Event ......................................................3-10

3.4 Using the Column Switching Template................................................. 3-12

3.5 Viewing Data....................................................................................... 3-14

3.6 Flushing the SCOUT Column Selector After Use................................. 3-15

Chapter 4 Maintenance and Troubleshooting

4.1 Maintenance......................................................................................... 4-2

4.2 Troubleshooting.................................................................................... 4-4

4.2.1 Front Panel LEDs ................................................................. 4-4

4.2.2 Error Codes ......................................................................... 4-5

Table of Contents

x Applied Biosystems

Appendix A Specifications

Appendix B Spare Parts

Appendix C Warranty/Service Information

C.1 Limited Product Warranty ..................................................................... C-2

C.2 Damages, Claims, Returns................................................................... C-5

Index

How to Use This Guide

SCOUT

®

Column Selector User Guide xi

2

How to Use This Guide

Purpose of this

guide

Applied Biosystems’

SCOUT

®

Column Selector User

Guide

contains the procedures for installing, running,

maintaining, and troubleshooting the SCOUT® Column

Selector.

Audience

This guide is intended for novice and experienced SCOUT

Column Selector users who are developing and executing

analytical and preparative separation protocols of

biomolecules based on POROS® Perfusion

Chromatography® media and conventional

chromatography media.

Structure of

this guide

Applied Biosystems’

SCOUT

®

Column Selector User

Guide

is divided into four chapters and three appendixes.

The table below describes the material covered in each

chapter and appendix.

Chapter Content

Chapter 1, Introducing the SCOUT

Column Selector Describes the features and parts of the SCOUT

Column Selector.

Chapter 2, Installing a Stand-Alone

SCOUT Column Selector Provides procedures for installing a stand-alone

SCOUT Column Selector hardware and software.

Chapter 3, Using the SCOUT

Column Selector Provides procedures for plumbing, configuring,

and using the SCOUT column selector, including

running a method, using the column switching

template, viewing Group Analysis, and flushing

the SCOUT column selector after use.

Chapter 4, Maintenance and

Troubleshooting Describes maintenance procedures and includes

troubleshooting information and error codes.

Appendix A, Specifications Includes system specifications.

How to Use This Guide

xii Applied Biosystems

2

Conventions

This guide uses the following conventions to make text

easier to understand.

General

conventions

•Bold text indicates user action. For example:

“Type 0 and press Enter for the remaining

fields.”

•

Italic

text denotes new or important words, and is

also used for emphasis. For example:

“Prior to operation,

always

check for

eluent miscibility.”

Notes, Cautions,

and Warnings

A Note calls out important information to the operator.

For example:

NOTE: Record your result before proceeding with the

next step.

A Caution provides information to avoid damage to the

system or loss of data. For example:

CAUTION

Do not touch the flow cell window. This may damage

the flow cell.

Appendix B, Spare Parts Includes ordering information for spare parts.

Appendix C, Warranty/Service

Information Includes warranty information.

Chapter Content

How to Use This Guide

SCOUT

®

Column Selector User Guide xiii

2

A Warning provides specific information essential to the

safety of the operator. For example:

WARNING

Always observe safe laboratory practices when

operating your system.

Remarques,

recommandations

et avertissements

Une remarque fournit une information importante à

l’opérateur er se présente ainsi:

REMARQUE: Si on vous demande d’insérer la

disquette de démarrage dans le lecteur, insérez-la puis

appuyez sur n’importe quelle touche.

Une recommandation fournit une information destinée à

éviter des détériorations du système ou la perte de

données:

RECOMMANDATION

La lampe peut être endommagée. N’y touchez pas.

Un avertissement fournit une information indispensable à

la sécurité de l’operateur et se présente ainsi:

AVERTISSEMENT

Conformez-vous toujours aux règlements du laboratoire

quand vous utilisez votre système.

Symbols used

on the system

The following symbol appears next to the values of the

fuses required by the system.

How to Use This Guide

xiv Applied Biosystems

2

Use this document in conjunction with the appropriate

document for your system:

•

Applied Biosystems’

BioCAD

®

Workstation for

Perfusion Chromatography User Guide

•

Applied Biosystems’

BioCAD

®

/ SPRINT

™

System

for Perfusion Chromatography User Guide

•

Applied Biosystems’

BioCAD

®

700E Workstation

for Perfusion Chromatography User Guide

•

Applied Biosystems’

VISION

™

Workstation for

Perfusion Chromatography User Guide

•

Applied Biosystems’

INTEGRAL

™

100Q System

User Guide

Send us your

comments

Applied Biosystems welcomes your comments and

suggestions for improving its manuals. You can send

your comments in two ways:

• Use the Technical Publications Customer Survey

at:

www.appliedbiosystems.com/perseptive/techsup/survey.htm

• Send e-mail to:

[email protected]

1

Chapter

SCOUT

®

Column Selector User Guide 1-1

1 Introducing

the SCOUT

Column Selector

This chapter includes the following sections:

1.1 SCOUT Column Selector Features ...........1-2

1.2 Parts of the SCOUT Column Selector .......1-3

Chapter 1 Introducing the SCOUT Column Selector

1-2 Applied Biosystems

1

1.1 SCOUT Column Selector

Features

Overview

Applied Biosystems’ SCOUT® Column Selector is an

automated multi-column switching unit for use with the

BioCAD® family of workstations and the INTEGRAL™ 100Q

System. The SCOUT Column Selector allows flow to be

diverted to any one of six attached columns.

The SCOUT Column Selector offers increased flexibility for

both systematic methods development and routine execution

of experiments.

A new template added to your system software allows you to

program columns to be switched sequentially or repeatedly

depending on your application.

Features

The SCOUT Column Selector provides the following features:

• Allows you to screen a range of columns and media to

systematically determine the effect of selectivity on the

desired separation

• Includes column clips that are compatible with columns

up to 24 mm in diameter and 30 cm in length

• Is compatible with ID™ Sensor Cartridges to allow

sequential peak tracking and Poroszyme™ Immobilized

Enzyme Cartridges to allow online enzymatic digestions

• On BioCAD, BioCAD 700E, and VISION systems, can

be plumbed at any position in the tandem column or

three column configuration to allow for automated multi-

dimensional chromatography methods development

Parts of the SCOUT Column Selector

SCOUT

®

Column Selector User Guide 1-3

1

1.2 Parts of the SCOUT

Column Selector

SCOUT models

This document describes two SCOUT models:

•Stand-alone—A free-standing unit available with

BioCAD/SPRINT™ Systems, BioCAD® Workstations,

BioCAD® 700E Workstations, and INTEGRAL™100Q

Multidimensional Biospecific HPLC Systems

(Figure 1-1).

Figure 1-1 Stand-Alone SCOUT Column Selector

•Built-in—Part of the VISION Workstation (Figure 1-2).

Figure 1-2 Built-In SCOUT Column Selector on VISION Workstation

PB100370

PB100512

SCOUT Column

Selector

Chapter 1 Introducing the SCOUT Column Selector

1-4 Applied Biosystems

1

Parts of the

SCOUT

The SCOUT Column Selector includes the following parts

(shown in Figure 1-3 and Figure 1-4):

•Column clips—Allow you to mount and secure

columns easily.

•Hinge (stand-alone model only)—Allows you to tilt the

column holder for easy access to the outlet ends of the

mounted columns.

• Valves—Top valve and bottom valve (not shown) allow

you to connect columns for switching.

• Front panel—Includes yellow Column LEDs that

indicate the column in use and a red Fault LED that

indicates a valve switching problem. The stand-alone

unit also includes a green Power LED that is lit when

the unit is on.

Figure 1-3 Stand-Alone SCOUT Column Selector

PB100370

hinge

valve

Column

clips

Column

holder

Front

panel Top

Column

holder

Parts of the SCOUT Column Selector

SCOUT

®

Column Selector User Guide 1-5

1

Figure 1-4 Built-in SCOUT Column Selector on VISION Workstation

PB100513

valve

Column

clips

Front

panel

Top

Chapter 1 Introducing the SCOUT Column Selector

1-6 Applied Biosystems

1

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