Thermo Fisher Scientific SureLock Tandem Midi Gel Tank Mode d'emploi

Marque
Thermo Fisher Scientific
Modèle
SureLock Tandem Midi Gel Tank
Taper
Mode d'emploi
For Research Use Only. Not for use in diagnostic procedures.
SureLock™ Tandem Midi Gel Tank
USER GUIDE
Catalog Number STM1001
Publication Number MAN0018507
Revision B.0
Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, California 92008 USA
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The information in this guide is subject to change without notice.
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LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018507
Revision Date Description
B.0 13 May 2021 Updating based on multiple instrument
changes.
A.0 02 July 2020 New manual.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
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©2021 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■SureLock Tandem Midi Gel Tank .................................................... 5
Product description ............................................................. 5
Product specifications ........................................................... 7
Overview of tank assembly ....................................................... 9
Gel electrophoresis protocol ..................................................... 10
Insert cassette ............................................................ 11
Fill tank with running buer and load samples ................................. 12
Start electrophoresis ....................................................... 12
Open midi gel cassette ......................................................... 13
Maintenance .................................................................. 16
Storage ....................................................................... 17
Run conditions ................................................................ 17
Troubleshooting ................................................................ 18
Related products .............................................................. 19
Customer and technical support ................................................. 22
■Tank safety ........................................................................... 23
■Safety ................................................................................. 25
Symbols on this instrument ..................................................... 25
Standard safety symbols ................................................... 26
Additional safety symbols ................................................... 26
Control and connection symbols ............................................. 27
Conformity symbols ........................................................ 27
Safety information for instruments not manufactured by Thermo Fisher Scientific ...... 28
Instrument safety .............................................................. 28
General ................................................................... 28
Electrical safety ........................................................... 29
Cleaning and decontamination .............................................. 30
Safety and electromagnetic compatibility (EMC) standards .......................... 30
Safety standards .......................................................... 31
Environmental design standards ............................................. 31
Chemical safety ................................................................ 32
SureLock™ Tandem Midi Gel Tank User Guide 3
■Limited product warranty ........................................................... 34
Contents
4SureLock™ Tandem Midi Gel Tank User Guide
SureLock Tandem Midi Gel Tank
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and gloves. Safety Data Sheets (SDSs) are available from
thermofisher.com/support.
Product description
The Invitrogen™ SureLock™ Tandem Midi Gel Tank provides a rapid protocol for midi gel electrophoresis
requiring minimal buer in a leak-free system. With a set-up time of ~30 seconds, the tank eciently
runs midi gels while providing consistent performance.
SureLock™ Tandem Midi Gel Tank User Guide 5
Table 1 Contents and part descriptions
Component Description
Midi Gel
Electrophoresis Tank
•Front and back design with
2 independent chambers each with
a capacity of ~550 mL.
•The cathode and anode
connectors are designed into the
electrophoresis tank.
Midi Gel Tank Lid
•Contains sockets for the anode and
cathode connectors.
•Connects to the power supply
through 2 electrode cables.
•Cable wrap feature to securely
wrap cables for easy storage.
•Lid and tank are keyed to ensure
proper installation.
Cassette Clamps (2) Each clamp consists of a cam plate and
2 independently movable cam handles.
SureLock Tandem Midi Gel Tank
Product description
6SureLock™ Tandem Midi Gel Tank User Guide
Table 1 Contents and part descriptions (continued)
Component Description
Gel Knife For easy opening of Invitrogen™ pre-
cast midi gels.
Product specifications
Specification Parameter
Dimensions (L × W × H) 25 × 18 × 17 cm (height with lid)
Capacity Up to 2 midi gels
Compatible Gels All Invitrogen™ precast midi gels and Invitrogen™ empty midi gel
cassettes
Gel Dimensions
Gel size: 8 × 13 cm
Gel cassette: 10.3 × 15 cm
Thickness: 1.0 mm
Buer Chamber Requirement Inner chamber: 170 mL (per gel)
Outer chamber: 350 mL (per gel)
Tank Material Polycarbonate
Electrode Wire Platinum (0.010" diameter)
Electrode Limits 300 VDC or 250 Watts
Operating Temperature 20-50°C
Temperature Limit 60°C
Chemical Resistance
Not compatible with chlorinated hydrocarbons (e.g., chloroform),
aromatic hydrocarbons (e.g., toluene, benzene), acetone, ethyl alcohol,
or isopropyl alcohol.
SureLock Tandem Midi Gel Tank
Product specifications
SureLock™ Tandem Midi Gel Tank User Guide 7
Two-prong plug
Lid
Electrode cables
Figure 1 Lid with electrode cables
3
5
5
4
1
2
6
6
Figure 2 Tank with cassette clamps
1Cathode Connector (–)
2Anode Connector (+)
3Cathode Electrode
4Anode Electrode
5Cassette Clamps
6Independent Gel Running Chambers
SureLock Tandem Midi Gel Tank
Product specifications
8SureLock™ Tandem Midi Gel Tank User Guide
Overview of tank assembly
1. Place the tank on a flat surface.
2. Place the cassette clamps in the chambers of the tank.
Note: The cassette clamps are directional, so ensure
they are placed in the correct orientation, as shown.
3. Place the midi gel cassette between the cassette
clamp and the gray sealing gasket with the cassette
wells facing the center of the tank.
4. Place the lid on top of the tank.
1
4
3
2
SureLock Tandem Midi Gel Tank
Overview of tank assembly
SureLock™ Tandem Midi Gel Tank User Guide 9
Gel electrophoresis protocol
Prepare buers
•Prepare 1X running buer appropriate for the type of midi gel being used. Approximately 520 mL of
1X running buer is needed for each gel run.
•Use the same buer type for both chambers of the electrophoresis tank (e.g., do not perform
electrophoresis with MES in one chamber and MOPS in the other).
Prepare samples
•Refer to the instructions for your midi gel for sample preparation guidelines including:
– Appropriate sample buers
– Recommended protein concentration and load volumes
Running conditions
•Prior to performing electrophoresis, ensure the power supply output is adequate for the number of
gels being run.
•Run times may vary depending upon the power supply.
•Refer to “Run conditions” on page 17 or the instructions for your midi gel for recommended
power settings.
Prepare gel cassette
Note: When handling a gel cassette, always hold by the edges.
1. Cut open the gel cassette pouch, discard packaging buer in sink, and gently remove the cassette.
2. Rinse the cassette with deionized water.
3. Remove the gel comb by sliding the comb up one side at a time, as shown below.
Note: Be careful not to damage the wells when removing the comb, or samples may leak from
one well to the other.
Gel comb
Gel cassette
SureLock Tandem Midi Gel Tank
Gel electrophoresis protocol
10 SureLock™ Tandem Midi Gel Tank User Guide
4. Remove the tape covering the slot at the lower portion of the cassette.
5. Using a pipette, rinse the wells 3 times with 1X running buer to remove any packaging buer from
the wells. Invert the gel and shake gently between rinses to remove buer.
CAUTION! Always wear protective clothing when performing laboratory experiments. To avoid
contamination from possible residual acrylamide, wear protective gloves when loading and unloading
the SureLock™ Tandem Midi Gel Tank, staining/destaining, and drying gels.
Insert cassette
1. Open the cassette clamps.
2. Place the gel cassette(s) between the cassette clamp
and the gray sealing gasket with the cassette wells
facing the center of the tank. Lower the cassette so it
rests on the bottom of the tank.
3. Close the cassette clamps.
SureLock Tandem Midi Gel Tank
Gel electrophoresis protocol
SureLock™ Tandem Midi Gel Tank User Guide 11
Fill tank with running buer and load samples
For each gel being run:
1. Add 1X running buer to the cathode (inner) chamber
(–) such that the gel wells are completely submerged
with buer (~170 mL).
2. Add 1X running buer to the anode (outer) chamber (+)
to the level of the red Fill Line printed on the outside of
the tank (~350 mL)
3. Load the samples and protein standards.
Note: Use caution to not damage the wells or gel with
the pipette tip during loading.
WARNING! If only running one gel, NEVER fill the unused chamber with buer. Operating
the tank with a chamber filled with buer, but without a clamped gel, can result in a short
circuit, excessively hot buer, and damage to the tank. See “Tank safety” on page 23 for more
information.
WARNING! NEVER fill the tank above the Fill Line with running buer. Overfilling the tank can
result in a short circuit, excessively hot buer, and damage to the tank. See “Tank safety” on
page 23 for more information.
Start electrophoresis
CAUTION! Make sure the power supply is o before connecting or disconnecting the Midi Gel Tank
to the power supply.
1. Place the lid on the electrophoresis tank. The lid can
only be firmly seated in one orientation. Match the red
and black circles on the electrodes within the tank to
the red and black electrodes on the lid.
Note: If the lid is not correctly seated, power will not
be properly supplied to the system.
2. With the power o, connect the electrode cables to
the power supply. Red to the (+) jack, Black to the (–)
jack.
WARNING! Ensure the electrode cable leads are inserted all the way into the sockets of the
power supply. Failure to do so can pose a shock hazard to the user. See “Tank safety” on
page 23 for more information.
SureLock Tandem Midi Gel Tank
Gel electrophoresis protocol
12 SureLock™ Tandem Midi Gel Tank User Guide
Note: If using a Bio-Rad™ Power Supply, Novex™ Power Supply Adapters (Cat. No. ZA10001) are
NOT needed for the SureLock™ Tandem Midi Gel Tank and should be removed prior to use.
3. Turn on the power supply.
4. Set the power supply according to the type of buer and gel being used (refer to “Run conditions”
on page 17 or the instructions provided with your midi gel). Press the Start button on the power
supply to begin electrophoresis.
WARNING! During electrophoresis of two gels, if one gel is removed from the tank in order to
run the second gel longer, the cassette clamp in the unused chamber MUST also be removed
before resuming electrophoresis (as per the warning label on the lid). Failure to do so can result
in a short circuit, excessively hot buer, and damage the tank. See “Tank safety” on page 23 for
more information.
Open midi gel cassette
1. End electrophoresis by pressing STOP on the power supply, or waiting until a run is completed.
2. Turn o the power supply and disconnect the cables.
WARNING! NEVER remove the tank lid or the electrode cables from the tank while the power
supply is active. See “Tank safety” on page 23 for more information.
3. Remove the lid and release the cassette clamps to the open position.
4. Remove the gel cassette(s) from the tank. Handle gel cassette(s) by the edges.
5. Lay the gel cassette (well-side up) on a flat surface.
6. Carefully insert the beveled edge of the Gel Knife into the narrow gap between the 2 cassette
plates, as shown below.
SureLock Tandem Midi Gel Tank
Open midi gel cassette
SureLock™ Tandem Midi Gel Tank User Guide 13
Note: Do not push the Gel Knife forcefully between the cassette plates or you may cut into the
gel.
7. Lever the Gel Knife handle up and down gently to separate the plates. A cracking sound indicates
that you have successfully broken the bond holding the cassette plates together.
8. Rotate the cassette and repeat steps 6–7 on the other sides until the 2 plates are completely
separated.
9. Upon separating the 2 plates, the gel may adhere to either side. Remove and discard the plate
without the gel. Allow the gel to remain on the other plate.
Note: Gels can easily tear if they are not handled carefully. Step 10 provides two methods for
removing the gel from the plate without tearing it.
SureLock Tandem Midi Gel Tank
Open midi gel cassette
14 SureLock™ Tandem Midi Gel Tank User Guide
10. Remove the gel from the plate using one of the 2 methods:
•Method 1: If the gel is adhered to the shorter (notched) plate, proceed with the following
steps:
a. Hold the Gel Knife at a 90° angle to the gel and push down to cut the gel (refer to diagram
below). Repeat the action laterally to cut o the entire foot.
b. Hold the plate with the gel facing downwards over a container filled with water. Use the
knife to carefully loosen one lower corner of the gel and allow the gel to peel away from
the plate and into the container.
•Method 2: If the gel is adhered to the taller (slotted) plate, proceed with the following steps:
a. Hold the plate with the gel facing downwards over a container filled with water.
b. Use the gel knife to push the gel foot through the slot in the cassette until the gel peels
away from the plate and gently falls into the container.
c. Cut the gel foot o the gel before transfer or after fixing and staining, but before drying.
Gel Knife
Taller slotted
plate
Gel foot
Cassette slot
11. Immediately fix, stain, or transfer the gel as desired.
SureLock Tandem Midi Gel Tank
Open midi gel cassette
SureLock™ Tandem Midi Gel Tank User Guide 15
Maintenance
WARNING! Disconnect the power cables before any cleaning or maintenance is performed on the
unit.
•When electrophoresis is complete, dispose of the buer appropriately. Rinse the tank with water to
remove residual running buer.
•Clean the surface of the Midi Gel Tank with a soft, non-abrasive, lint-free cloth dampened with
water.
•The gel tank material is not compatible with chlorinated hydrocarbons (e.g., chloroform),
aromatic hydrocarbons (e.g., toluene, benzene, etc.), acetone, ethyl alcohol, or isopropyl
alcohol.
WARNING! Cleaning the Midi Gel Tank with non-compatible chemicals can lead to tank
fracturing and leaks.
For any issues, contact Technical Support (see “Customer and technical support” on page 22).
SureLock Tandem Midi Gel Tank
Maintenance
16 SureLock™ Tandem Midi Gel Tank User Guide
Storage
•Allow tank to dry completely before storing.
•Do not store with buer or gels inside the tank.
•Cassette clamps may be stored inside the tank.
•For ease of storage, use the cable wrap hook on the lid. The cable wraps around the lid and the
end of the cable features a clip that keeps it in place.
Cable wrap
hook
Run conditions
Expected run conditions during electrophoresis are provided for various types of midi gels. The current
values in the table below are for one gel. If running two gels, the current would be doubled. Note that
current and run-time values are approximate and will vary depending on gel percentage and the power
supply used for electrophoresis.
Gel Type Constant
Voltage (V)
Starting Current
(mA)
Maximum
Current (mA)
Run Time
(minutes)
4-12% Bis-Tris
(MES buer) 200 200 300 30
4-12% Bis-Tris
(MOPS buer) 200 190 210 40
3-8% Tris-Acetate (Denatured) 150 60 80 60
3-8% Tris-Acetate (Native) 150 40 60 135
4-20% Tris-Glycine Plus
(Denatured) 200 90 100 60
4-20% Tris-Glycine Plus (Native) 125 40 50 120
SureLock Tandem Midi Gel Tank
Storage
SureLock™ Tandem Midi Gel Tank User Guide 17
Troubleshooting
Observation Possible cause Recommended action
Run takes longer than normal. Buers were too dilute. Check buer recipes. Do not reuse buers.
Remake buer if necessary.
Buer chamber was leaking. Make sure the cassette clamp is firmly seated,
the gaskets are in place, and the cassette
clamps are locked.
Voltage and/or current was set
too low.
Set the correct voltage and/or current. See
"Running conditions" in “Gel electrophoresis
protocol” on page 10 for more details.
Run is faster than normal with
poor resolution.
Incorrect running buer was
used or buer was too
concentrated.
Check buer recipe. Dilute or remake buer if
necessary.
Voltage and/or current was set
too high.
Decrease voltage and/or current to
recommended running conditions. See "Running
conditions" in “Gel electrophoresis protocol” on
page 10 for more details.
Current reading on power
supply is zero or very low.
Tape was left on the bottom of
the cassette.
Remove the tape from the cassette.
Connection to power supply
was not complete.
Check conductance on all connections using a
voltmeter.
Note: Do not check connections if not trained
in this procedure. Contact Technical Support for
further help.
Insucient buer level. Make sure there is sucient buer in the tank.
The cathode (inside) chamber needs to be filled
above the wells (~170 mL), and the anode
(outside) chamber needs to be filled to the red fill
line (~350 mL). See “Fill tank with running buer
and load samples” on page 12 for more details.
Cannot see the sample wells to
load the sample.
Dicult to see contrast between
the sample well and the rest of
the gel.
Mark cassette at the bottom of the wells with a
marker pen prior to placing the cassette in the
electrophoresis tank.
Current reading on power
supply is much higher than
expected or maxed out.
Too many gels were being run at
once using one power supply.
Check the power limits of the power supply
being used and use additional power supplies
if needed.
Cassette was not properly
clamped.
Ensure that the cassette clamp is firmly seated,
the gaskets are in place, and the cassette
clamps are in the locked position. See “Insert
cassette” on page 11 for more details.
SureLock Tandem Midi Gel Tank
Troubleshooting
18 SureLock™ Tandem Midi Gel Tank User Guide
Observation Possible cause Recommended action
Current reading on power
supply is much higher than
expected or maxed out.
(continued)
Tank was overfilled with running
buer.
If buer level is above the height of the gel,
the cathode and anode chambers are in direct
contact resulting in an electrical short circuit.
Ensure correct buer volumes. Cathode buer
level should be covering the wells but not above
the height of the gel (~170 mL). Anode buer
level should be filled to the red fill line printed
on the outside of the tank (~350 mL). See “Fill
tank with running buer and load samples” on
page 12 for more details.
Proteins are not migrating into
the gel.
The cassette is installed
backwards in the tank.
Install the cassette in the correct orientation,
with the well opening facing towards the
cathode electrode (center of the tank).
The power cable is installed
backwards into the power
supply.
Install the power cable in the correct orientation
(red to red, black to black).
Related products
Replacement parts, related products and consumables can be found in the tables below.
Table 2 Replacement parts
Product Quantity Product No.
SureLock™ Tandem Midi Gel Tank 1 STM1003
SureLock™ Tandem Midi Gel Tank Replacement Lid 1 STM1002
SureLock™ Tandem Midi Gel Tank Replacement Cassette Clamp 1 STM1004
Gel Knife 1 EI9010
Table 3 Power Supplies
Product Quantity Product No.
PowerEase™ Touch 350 W Power Supply (115 VAC) 1 PS0350
PowerEase™ Touch 350 W Power Supply (230 VAC) 1 PS0351
Table 4 Pipette tips for gel loading
Product Quantity Product No.
Gel Loading Tips (Standard Round) 200/pk LC1001
Flat Gel Loading Tips 200/pk LC1002
SureLock Tandem Midi Gel Tank
Related products
SureLock™ Tandem Midi Gel Tank User Guide 19
Table 5 Pre-mixed buers
Product Quantity Product No.
NuPAGE™ Midi Gels (Bis-Tris and Tris-Acetate)
NuPAGE™ MOPS SDS Running Buer (20X) 500 mL NP0001
NuPAGE™ MES SDS Running Buer (20X) 500 mL NP0002
NuPAGE™ Tris-Acetate SDS Running Buer (20X) 500 mL LA0041
NuPAGE™ Sample Reducing Agent (10X) 10 mL NP0009
NuPAGE™ Antioxidant 15 mL NP0005
NuPAGE™ LDS Sample Buer (4X) 250 mL NP0008
Novex™ Tris-Glycine Midi Gels
Novex™ Tris-Glycine SDS Running Buer (10X) 500 mL LC2675
Novex™ Tris-Glycine SDS Sample Buer (2X) 20 mL LC2676
Novex™ Tris-Glycine Native Running Buer (10X) 500 mL LC2672
Novex™ Tris-Glycine Native Sample Buer (2X) 20 mL LC2673
Table 6 Pre-cast gels
Product Quantity Catalog No.
Novex™ Tris-Glycine Plus Midi Gels
Novex™ 10%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 12+2-well 10 gels WXP01012BOX
Novex™ 10%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 20-well 10 gels WXP01020BOX
Novex™ 10%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 26-well 10 gels WXP01026BOX
Novex™ 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 12+2-well 10 gels WXP01212BOX
Novex™ 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 20-well 10 gels WXP01220BOX
Novex™ 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 26-well 10 gels WXP01226BOX
Novex™ 4 to 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 12+2-well 10 gels WXP41212BOX
Novex™ 4 to 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 20-well 10 gels WXP41220BOX
Novex™ 4 to 12%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 26-well 10 gels WXP41226BOX
Novex™ 4 to 20%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 12+2-well 10 gels WXP42012BOX
Novex™ 4 to 20%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 20-well 10 gels WXP42020BOX
Novex™ 4 to 20%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 26-well 10 gels WXP42026BOX
Novex™ 8 to 16%, Tris-Glycine Plus, 1.0 mm, Midi Protein Gel, 12+2-well 10 gels WXP81612BOX
SureLock Tandem Midi Gel Tank
Related products
20 SureLock™ Tandem Midi Gel Tank User Guide
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Thermo Fisher Scientific SureLock Tandem Midi Gel Tank Mode d'emploi

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Modèle
SureLock Tandem Midi Gel Tank
Taper
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