Phcbi MCO-230AIC Mode d'emploi

Operating Instructions

Please read the operating instructions carefully before using this product, and keep the operating

instructions for future use.

See page 71 for model numbers.

CO2 Incubator

2

CONTENTS

INTRODUCTION .......................................................................................... 4

INTENDED USE AND PRECAUTIONS ........................................................ 4

PRECAUTIONS FOR SAFE OPERATION ................................................... 5

LABELS ON INCUBATOR .......................................................................... 10

SYMBOLS ON INCUBATOR ...................................................................... 11

ENVIRONMENTAL CONDITIONS .............................................................. 11

INCUBATOR COMPONENTS .................................................................... 12

Unit .......................................................................................................... 12

LCD touch panel ...................................................................................... 14

Remote alarm terminal ............................................................................ 16

INSTALLATION ........................................................................................... 17

Installation site ......................................................................................... 17

Installation ............................................................................................... 18

Connecting a CO2 gas cylinder ............................................................... 20

BEFORE COMMENCING OPERATION ..................................................... 21

Initial cleaning method............................................................................. 21

Removing inner attachments ................................................................... 22

Installing inner attachments ..................................................................... 24

Filling the humidifying pan ....................................................................... 25

FOR OPTIMAL CULTIVATION .................................................................... 26

Precautions for cultures........................................................................... 26

Preventing contamination ........................................................................ 27

CORRECT OPERATION ............................................................................ 28

BASIC OPERATION ON LCD TOUCH PANEL .......................................... 29

Operation from Menu key ........................................................................ 29

Entering numeric values into input window ............................................. 31

Operating slide keys ................................................................................ 32

BASIC PARAMETERS ................................................................................ 33

Setting temperature, CO

2

density, and high-limit temperature alarm

........... 33

Setting key lock ....................................................................................... 34

Turning off key lock ................................................................................. 34

ALARM PARAMETERS .............................................................................. 35

OPERATION/ALARM LOG ......................................................................... 37

Setting log interval ................................................................................... 37

Displaying operation log .......................................................................... 38

Exporting operation log ........................................................................... 40

Displaying alarm log ................................................................................ 42

Exporting alarm log ................................................................................. 43

OTHER PARAMETERS .............................................................................. 45

Setting date and time .............................................................................. 45

Setting brightness and sleep ................................................................... 46

3

CONTENTS

UV LAMP PARAMETERS .............................................................................................. 47

Using the UV lamp ..................................................................................................... 47

Setting UV lamp ON period ........................................................................................ 48

Lighting the UV lamp for 24 hours .............................................................................. 49

H2O2 DECONTAMINATION (OPTIONAL) ..................................................................... 50

H

2O2 decontamination ................................................................................................ 50

Precautions when handling H2O2 reagent .................................................................. 54

ELECTRIC LOCK (OPTIONAL) ..................................................................................... 55

Setting User-ID ........................................................................................................... 55

Setting auto-lock ......................................................................................................... 56

Using unlock key ........................................................................................................ 58

Turning off auto-lock ................................................................................................... 58

GAS AUTO-CHANGER (OPTIONAL) ............................................................................ 59

Connecting CO2 gas cylinders ................................................................................... 59

Automatic CO2 gas supply line changeover ............................................................... 59

Manual CO2 gas supply line A/B changeover ............................................................ 61

STD GAS AUTO-CALIBRATION KIT (OPTIONAL) ....................................................... 62

ALARMS, SAFETY, AND SELF-DIAGNOSIS ................................................................ 64

TROUBLESHOOTING ................................................................................................... 67

SPECIFICATIONS ......................................................................................................... 69

PERFORMANCE ........................................................................................................... 71

SAFETY CHECK SHEET .............................................................................................. 72

English

4

INTRODUCTION

Ŷ Read the operating instructions carefully before using the product and follow the instructions for safe

operation.

Ŷ PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with

any procedures other than those given in the operating instructions.

Ŷ Keep the operating instructions in a suitable place so that they can be referred to as necessary.

Ŷ The operating instructions are subject to change without notice for improvement of performance or

function.

Ŷ Contact our sales representative or agent if any page of the operating instructions is lost or the page

order is incorrect, or if the instructions are unclear or inaccurate.

Ŷ No part of the operating instructions may be reproduced in any form without the express written

permission of PHC Corporation.

IMPORTANT NOTICE

PHC Corporation guarantees this product under certain warranty conditions. However, please note that

PHC Corporation shall not be responsible for any loss or damage to the contents of the product.

INTENDED USE AND PRECAUTIONS

This equipment is designed for culture of cells, tissues, organs, and embryos.

Ŷ The adapted culture condition depends on the kind of sample. It is necessary to determine the culture

temperature, CO2 density, and culture period that is required.

Ŷ For culture of embryos, low O2 density is desirable. Using an O2/CO2 incubator is recommended.

Ŷ For IVF/ART use, special attention should be paid to traceability, since incidents may be disclosed

several months or years later, at the baby’s birth, or during its later life. It is therefore recommended that

the following data is maintained: product serial number, incubation term, and incubation parameters.

(Refer to details on MEDDEV 2.2/4.)

5

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with the operating instructions

as they contain important safety advice.

Items and procedures are described so that this unit can be used correctly and safely.

Following these precautions will prevent possible injury to the user and any other person.

Precautions are illustrated in the following way:

WARNING

Warning indicates a potentially hazardous situation which, if not avoided,

could result in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and

damage to the unit and associated property.

Symbols have the following meaning;

㻌 This symbol means caution.

㻌 T

his symbol means an action is prohibited.

㻌 T

his symbol means an instruction must be followed.

Be sure to keep the operating instructions in a place that is accessible to users of this

unit.

WARNING

As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity

of the equipment. It is important that you assess the work site to ensure there is suitable and

sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a

safe environment must be considered. These may include atmosphere monitoring and warning

devices.

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6

PRECAUTIONS FOR SAFE OPERATION

Do not use the unit outdoors. Exposure to rain may cause leakage and/or electric shock.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit in a location capable of bearing the total combined weight (product + optional

accessories + stored items). After installing the unit, be absolutely sure to take precautions to

prevent the unit from falling over. If the unit is installed in a location which is not strong enough or if

the proper precautions are not taken, the unit may fall over and cause injuries.

Do not install the unit where there are high levels of moisture or where it may be splashed with

water. Installing the unit where there are high levels of moisture or where it may be splashed with

water may cause the insulation to deteriorate and give rise to leakage and/or electric shock.

Do not install the unit in a location where flammable or volatile substances are present.

Installing the unit in a location where flammable or volatile substances are present may cause

explosions and/or a fire.

Do not install the unit in a location where corrosive gases such as acids are present. Installing

the unit in a location where corrosive substances are present may cause electric components to

corrode, leading to leakage and/or electric shock due to the deterioration of insulation resulting from

corroded electrical components.

Do not place this unit in a location where it is difficult to disconnect the power supply plug.

Failure to disconnect the power supply plug may cause fire in the event of a problem or malfunction.

Be absolutely sure to earth (ground) the unit in order to prevent electric shock. Failure to

earth the product may give rise to electric shock. If necessary, ask a qualified contractor to do this

work.

Do not connect the earth wire to a gas pipe, water pipe, or lightning rod when earthing the

unit. Earthing the unit improperly may give rise to electric shock.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit except in a sealed container.

Such substances may cause explosion or fire if they leak.

Never insert metal objects such as pins and wires into any vent, gap or outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

When handling harmful samples (for example, those which consist of toxic, pathogenic,

or radioactive substances), install the unit inside a designated isolation facility. If the

unit is installed in a location which is not an isolation facility, there may be detrimental effects on

both people and the natural environment.

WARNING

7

Before proceeding with maintenance or checking of the unit, set the power switch to OFF and

disconnect the power supply plug. Performing the work while power is still flowing to the product or

while the power-supply plug is still connected may give rise to electric shock and/or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

Wear protective gloves and mask during maintenance. Touching or inhaling chemicals or

aerosols from around the unit may be detrimental to health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on top of the unit as this may cause electric shock or short circuit if

the liquid is spilled.

Never damage the power supply cord or power supply plug (by breaking, adapting, placing near

a source of heat, bending with force, twisting, pulling, adding weight, or binding). A damaged

power supply cord or power supply plug may cause electric shock, short circuit, or fire.

Never disassemble, repair, or modify the unit yourself. A high-voltage area is located inside the

unit. Any work carried out by an unauthorized personnel may result in electric shock. Contact our

sales representative or agent for maintenance or repair.

Make sure the power supply plug is pushed fully in. Faulty insertion of the power supply plug may

cause electric shock or fire due to generation of heat. Never use a damaged power supply plug or

loose power outlet.

Disconnect the power supply plug if there is anything wrong with the unit. Continued abnormal

operation may cause electric shock or fire.

Grip the power supply plug when disconnecting the power supply cord from the outlet. Pulling

the power supply cord may cause electric shock or short circuit.

Remove dust from the power supply plug periodically. Dust on the power supply plug may cause

insulation failure due to moisture and thus cause a fire. Disconnect the power supply plug and wipe it

with a dry cloth.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

supply cord. A damaged cord may cause electric shock or fire.

Disconnect the power supply cord when the unit is not in use for long periods. Keeping the unit

connected may cause electric shock, leakage, or fire due to the deterioration of insulation.

WARNING

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8

PRECAUTIONS FOR SAFE OPERATION

If the unit is to be stored unused in an unsupervised area for a long period, ensure that children do

not have access and that doors cannot be closed completely.

Ask a qualified contractor to carry out disassembly and disposal of the unit. Leaving the unit in

a location that can be accessed by third parties may result in unexpected accidents (e.g. the unit may

be used for unintended purposes).

Do not leave the plastic bags used for packing in a place where they can be reached by small

children as this may result in unexpected accidents such as suffocation.

Use the reagent specified by our company for H2O2 decontamination. Using a different H2O2

solution may result in explosion or damage to the incubator.

When performing H2O2 decontamination, securely close the internal and external doors. Failure to

do so may be harmful to health due to leakage of H2O2 gas.

During H2O2 decontamination, plug the access hole with the silicon cap that is provided. Failure to

do so may be harmful to health due to leakage of H2O2 gas.

Always use the removal power supply cord that is provided. Other power supply cord may cause

electric shock or fire.

When using CO2 gas for control, make sure that there is adequate ventilation. Using CO2 gas in a

small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,

when opening the incubator doors, do not directly inhale the air in the chamber.

When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the

connections are secure and that no gas will leak. Be sure to use the specified pressure. Using

an incorrect gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation

due to gas leak.

Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be

provided, then install an alarm system using CO2 and O2 densitometers.

Do not look directly at UV light. UV light is harmful to the eyes.

Never start H2O2 decontamination when “Warning: UV Bulb Life” is displayed in the message display

field. The UV resolve is not sufficient.

Do not use the unlock key to unlock the outer door during H2O2 decontamination or during H2O2

gas resolve by UV. Doing so may cause harm to health from H2O2 gas leakage.

The CO2 incubator must be dismantled and disposed of by qualified personnel only. If the CO2

incubator is left where outsiders enter, it may result unexpected accident (for example, children to

become locked inside).

WARNING

9

This unit must be plugged into a dedicated circuit protected by branch circuit breaker.

Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may

cause fire resulting from abnormal heating.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for

the safety of service personnel.

Wear rubber gloves when handling the H2O2 reagent. Direct contact with the H2O2 reagent may

result in inflammation of the skin.

H2O2 decontamination can be performed only for the chamber and chamber attachments with standard

specifications, and not for any other objects.

Perform H2O2 decontamination with the chamber attachments arranged as specified by our

company. Arranging them in a different way may result in insufficient decontamination.

After H2O2 decontamination has been completed, wear rubber gloves and use a non-woven cloth to

wipe off the residual H2O2 fluid from the bottom of the chamber, any objects that were

decontaminated, and the bottoms of ducts.

Do not climb on top of the incubator or place any object on it. By doing so you may damage the

incubator or knock it over, which may result in injury.

Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may

result in cuts or abrasions from sharp edges or corners.

CAUTION

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10

LABELS ON INCUBATOR

Users are advised to read carefully the warnings and cautions contained on stickers at key locations on the

interior and exterior of the incubator.

Possible

Danger Warning/Caution Type

Location of Danger Warning/Caution Label Description of Danger

Burns Hot Surface

Cooling Unit &

Heat Cover

Avoid touching the cooling unit

and heat cover, which can reach

high temperatures and may cause

burns.

Personal

injury Hazardous UV Light

Interior The UV lamp is illuminated by

pressing the door switch. Do not

press the door switch: the UV light

is hazardous.

Personal

injury Hazardous UV Light

Interior

The UV light is hazardous. Never

turn on the UV lamp without the

cover.

Personal

injury Gas Poisoning or

Oxygen Deprivation

Environment

When using CO2 gas, ensure

there is adequate ventilation.

Using CO2 gas in a small room

without adequate ventilation may

cause gas poisoning or oxygen

deprivation. In addition, when

opening the incubator doors, do

not directly inhale the air in the

chamber.

Personal

injury Gas Poisoning or

Oxygen Deprivation

Interior

Excessive pressure may cause

gas supply lines inside the

incubator to come loose, which

may result in gas poisoning or

oxygen deprivation due to the

escape of gas.

Personal

injury Electric shock

Top cover Never remove this cover. Only a

service personnel remove the

cover to avoid the electric shock.

11

SYMBOLS ON INCUBATOR

The following symbols are attached to the incubator:

Attached to covers that access high-voltage electrical components to prevent electric

shock. Only a qualified engineer or service personnel should be allowed to open

these covers.

Indicates an ultraviolet light (UV) caution.

Indicates that caution is required. Refer to precautions for safe operation for details.

Indicates a hot surface.

Indicates an earthing.

Indicates “ON” for a power switch.

Indicates “OFF” for a power switch.

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):

Ŷ Indoor use;

Ŷ Altitude up to 2000 m;

Ŷ Temperature 5 oC to 40 oC;

Ŷ Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative

humidity at 40 oC;

Ŷ Mains supply voltage fluctuations up to ±10 % of the nominal voltage;

Ŷ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;

Ŷ Temporary OVERVOLTAGES occurring on the mains supply;

Ŷ Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);

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12

INCUBATOR COMPONENTS

Unit

1 2 3 Tray 4 5

6

7*1

8

13

12*

2 Electric key*2 11 10 9

*1: MCO-230AICUV or when an optional UV system

set MCO-170UVS is installed.

*2: When an optional electric lock MCO-170EL is

installed.

*3: Type FG-7P or FG-7PL.

19

Leveling

feet Leveling

Foot

Handles

(When some inner

attachments are removed)

(When 13 is removed)

Sample air outlet cap

18 17 16*3 15 14

Lower right side

Removable power

supply cord port

20

21

Access port

cover Silicon cap

Rear side

13

1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in

the door panel. The door opening is reversible. Contact our sales representative or agent to change the

door hinge from left to right or vice versa.

2. Inner door: The inner door is made of tempered glass. Do not subject the glass to excessive impact.

3. Tray catches: Insert tray to fit the concave portion on the chamber.

4. Fan cover (above the duct): Serves as the inlet for circulating air. It is removable.

5. Duct: The duct for the path for circulating air. It is removable.

6. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.

7. UV lamp*1:This UV lamp does not generate ozone. For replacement, contact our sales representative

or agent.

8. Humidity control bar: Automatically reduces condensation caused by the external environment when

the door is frequently opened and closed. The humidity control bar has a bactericidal effect due to its

plated surface. However, it is recommended that it is replaced every 5 to 6 years to maintain this effect

(The duration of bactericidal effect differs depending on the environment).

9. Humidifying pan cover: Prevents UV light entering the chamber.

10. Humidifying pan: Fill with sterile distilled water.

11. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO2

when the door is open. The UV lamp*1 is also deactivated by the door opening.

12. Key hole*2: This is unlocked with the unlock key when the outer door is locked by the electric lock.

13. Switch cover: Prevents shutting down of the unit in case of accidental pressing of the main power

switch.

14. Power supply cord cover plate: Prevents the power supply cord becoming detached.

15. Connecting port A for CO2 gas pipe: Refer to page 20 for gas cylinder connection.

Note: When the optional MCO-21GC gas auto-changer is installed, both ports A and B are available. Refer

to page 59.

16. Glow starter*3:Starts the glow for the UV lamp.

17. Power switch: This is the main switch for the incubator (ON-“I”, OFF-“O”). It also functions as an

overcurrent breaker.

18. Sample air outlet: This also functions as an internal gas outlet. In normal use, cover this outlet with

the sample air outlet cap.

19. USB port: Insert USB memory device to export operations and alarms log. Refer to pages 40–44.

20. Access port: Place the silicon caps on both the outside and inside of the port when the port is not

being used.

21. Remote alarm terminal: Sends the alarm to a remote location by connecting to an external alarm unit.

Refer to page 16.

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14

INCUBATOR COMPONENTS

LCD touch panel

The following display (called the Top screen) will appear when the power switch is turned ON.

Note: It takes approximately 20 seconds until the Top screen is displayed. During warming-up, “Status:

Gas sensor initializing” is displayed in the Message display field (13), and “--.-” is displayed in the present

CO2 density display field (4).

2 6

㻌㻌

7 8

3

1

1. Present temperature display field

The current chamber temperature is displayed.

2. Set temperature value display field

The set value of chamber temperature is

displayed. Default setting: 37 oC

3. Heating indicator

This lights up when the heater is on.

4. Present CO2 density display field

The current chamber CO2 density is displayed.

Nothing is displayed when CO2 density is set to

0 %.

5. Set CO2 density value display field

The set value of the chamber CO2 density is

displayed. Default setting: 0 %.

6. CO2 gas injection indicator

This lights up when CO2 gas is being injected.

7. CO2 gas supply line indicator A and B*

Current supplying CO2 gas supply line

(connecting port for CO2 gas pipe) is displayed.

When the CO2 gas automatic changer function

changes the empty CO2 gas cylinder to the

other, the empty indicator is displayed in reverse

video and blinks.

8. CO2 gas supply line select key*

This key selects CO2 gas supply line A or B

(connecting port A or B for CO2 gas pipe). When

an optional gas auto-changer MCO-21GC is

installed, CO2 gas supply line A/B changes over

automatically when CO2 gas cylinder is empty.

* Only displayed when optional component MCO-21GC (gas auto-changer) is installed.

5

4

15

10 11 9 12

13

18 17 16 15

9. Present date/time display field

The date and time is set when the incubator is

shipped from the factory. Refer to page 45 for

details.

10. Over heat display

High-limit temperature alarm is activated:

“Over Heat” is displayed alternately in normal

characters and reverse video.

11. UV lamp condition display

UV lamp ON: “UV : On” is displayed.

UV lamp OFF: “UV : Off” is displayed.

Note: Displayed only when optional UV system

set MCO-170UVS is installed to the

MCO-230AIC.

12. Outer door (opening/closing) display

Open: “Door : Open” is displayed alternately in

normal characters and reverse video.

Closed: “Door : Closed” is displayed.

Locked: “Door : Locked” is displayed.

13. Message display field

Alarms, errors, or messages are displayed

when a fault occurs. Refer to pages 64–66.

Note: When there are several errors, the display

shows the message number. For example, if

there are two errors, the display shows “1/2”.

14. Message select key

When there are several alarms/errors, the

message on the screen can be changed.

15. Menu key

Press this key to access the Menu screen. Refer

to page 29.

16. H2O2 key

This key initiates H2O2 decontamination. Refer

to pages 50–54. This key is displayed if the

incubator is equipped for H2O2 decontamination.

17. Unlock key

Press this key to unlock the outer door when it is

auto-locked by the electric lock. Refer to page

57. This key is displayed when an optional

electric lock MCO-170EL is installed and the

auto-lock function is ON.

18. Buzzer key

Press this key to silence the buzzer. Refer to

pages 36 and 64–66.

14

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16

INCUBATOR COMPONENTS

Remote alarm terminal

The alarm of this product can be transferred to a remote location by connecting the external alarm unit to

the remote alarm terminal. For the behaviour of remote alarm output, refer to pages 64–66.

The terminal of the remote alarm is installed at the

rear upper right of the unit (see the figure opposite).

The alarm outputs from this terminal. Contact

capacity is DC 30 V, 2 A.

Table 1 shows the behaviour of the remote alarm

when the Buzzer key is pressed.

Notes:

• The door alarm does not work remotely. Refer to

pages 64–65.

• Wiring of the remote alarm should be performed by

qualified service personnel.

Table 1. Behaviour of remote alarm when pressing Buzzer key

Remote Alarm setting

(Refer to pages 35–36) Connecting

terminal Normal

condition

Abnormal condition

(Including in the cases of power outage and

of where the power supply plug is pulled out)

When pressing the buzzer key

ON: Remote alarm setting not

connected with Buzzer key

(Remote alarm is cancelled)

COM.-N.C. Close Open Open (Maintain in abnormality)*

COM.-N.O. Open Close Close (Maintain in abnormality)*

OFF: Remote alarm setting

connected with Buzzer key

(Remote alarm is not

cancelled)

COM.-N.C. Close Open Close (Return to normal)

COM.-N.O. Open Close Open (Return to normal)

* In the case of Err01 (CO2 gas cylinder empty), Err11 and Err12 (CO2 sensor error), the condition returns

to normal.

Remote alarm

terminal

Use a twisted shield wire for the connection.

Type: UL 2343, UL 2448, UL 2464, UL 2552, UL2623.

Length: 30 m max.

17

INSTALLATION

Installation site

For correct operation, install the incubator in a location with the following conditions (referring also to the

precautions for safe operation on pages 5–9):

z

Install in an environment with normal air.

z

Do not expose to direct sunlight.

z

Do not install near significant heat sources, such as heaters, boilers, ovens, or autoclaves.

z

Ensure ambient temperature is at least 5

q

C lower than set temperature.

z

Do not allow the ambient temperature to become too high.

z

Install in a site with a relative humidity of 80 %R.H. or lower.

z

Do not expose to flammable or corrosive gas.

z

Do not install where objects may fall on the incubator from above.

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18

INSTALLATION

Installation

1. Remove the packing tape and clean up.

Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.

If the outer panels are dirty, use a cloth to wipe them with a diluted neutral detergent (Undiluted detergent

can damage the plastic components. For the dilution, refer to the instructions on the detergent).

Wipe off the residual detergent with a wet cloth and then wipe off any moisture.

Note: Remove the cable tie around the power supply cord to prevent corrosion of the cord coating.

2. Set up the humidifying pan and humidifying pan cover (Fig. 1).

3. Install 4 trays (Fig. 2).

4. Adjust the levelling feet.

Adjust the levelling feet by turning them

anticlockwise until the incubator is level (Fig. 3).

Note: Incubating on a sloping tray may adversely

affect the cultivation.

5. Earth the incubator.

Earth the incubator during installation to prevent electric shock. If there is no earth wire at the location,

consult with qualified service personnel.

Fig. 1 Fig. 2

4 Trays

Humidifying pan

Humidifying pan cover

Fig. 3

Leveling feet

Shrink

Stretch

19

z

When an earth must be installed

If an earthed 3-pole outlet is not available, then an earth must be installed. Consult with qualified service

personnel.

z

Installing an earth fault circuit breaker

If you must use the incubator in a moist or humid location, then it is recommended that an earth fault circuit

breaker be installed in the power supply circuit (i.e., the power supply at the incubator). Have the circuit

breaker installed by qualified service personnel.

z

Double-stacking

For stacking the incubators securely, refer to the

procedure included with the optional

double-stacking bracket MCO-170PS or the

stacking plate MCO-230SB.

Note: Two hooks are attached at the upper rear of

the incubator. When stacking incubators, fix the

upper incubator to the wall with these hooks and

wire or chain (Fig. 4).

Note: When stacking the incubators with our CO2

incubator or another O2/CO2 incubator, use the

stacking plate MCO-230SB. Refer to Table 11 and

12 on page 70.

z

When the incubator is not in use

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the

chamber is completely dry before closing the doors. Failure to do so may result in damage.

z

Before moving the incubator

Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug

from the outlet, and make sure that the power supply cord will not be damaged. Failure to do so may result

in electric shock or fire.

z

If the key is fitted in the door lock

Remove the key from the door lock and keep it in a secure place. Make a note of the key symbol and

number in case the key is lost. To remove the key from the door lock, open the outer door and turn the key

in the direction of the lock. Remove the key and close the door.

Note: The outer door can only be locked with the unlock key while the outer door is open. Attempting to

turn the unlock key while the outer door is closed may damage the electric lock system.

Fig. 4

Hooks

Wire

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20

INSTALLATION

Connecting a CO2 gas cylinder

Before connecting a CO2 gas cylinder, read the precautions for safe operation on pages 5–9.

1. Get a CO2 gas cylinder ready and install an optional gas regulator MCO-010R.

Notes:

• Use a liquefied-CO2 gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.

• When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm2(G),

3600 psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm2(G), 36psi(G)) for the secondary side.

2. Using the gas tube provided, connect the

connecting port A for the CO2 gas pipe and the gas

regulator of the CO2 gas cylinder.

Notes:

• By using two tube bands provided, connect the gas tube tightly to prevent it from coming off.

• Make sure that the gas tube is not folded.

•If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder, a CO2 solid will be formed

in the gas regulator. The gas regulator safety valve will operate, and there may be an explosive sound.

3. After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detector spray).

4. Set the CO2 gas on the secondary side to 0.03 MPa(G)–0.1 MPa(G) (0.3 kgf/cm2(G)–1 kgf/cm2(G),

4.4 psi(G)–14.5 psi(G)) for gas injection. Recommended pressure: 0.03 MPa(G) (0.3 kgf/cm2(G), 4.4

psi(G)).

Note: As the pressure increases, the CO2 gas density variation will increase. Excessive pressure may

cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen

deprivation due to leaking gas. If gas lines come loose, the incubator must be repaired.

5. When there is no CO2 gas left and the CO2 gas empty alarm is activated, replace the empty gas cylinder.

Note: When the optional gas auto-changer MCO-21GC is installed, it switches the empty CO2 gas supply

line to the other automatically. Refer to pages 59–60.

Note: The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities

are found during inspection, replace the lines immediately.

Connecting port A for CO2 gas pipe

Lower right side

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