Thermo Fisher Scientific RapidFinder Poultry ID Kit Mode d'emploi

Marque: Thermo Fisher Scientific

Modèle: RapidFinder Poultry ID Kit

Taper: Mode d'emploi

For testing of Food and Environmental samples only.

RapidFinder™ Poultry ID Kit

USER GUIDE

Real-time PCR detection of poultry DNA in food and feed

samples

for use with:

Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument with Thermo

Scientiﬁc™ RapidFinder™ Analysis Software v1.2 or later

Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument with Applied

Biosystems™ RapidFinder™ Express Software v2.0 or later

Catalog Number A24397

Publication Number MAN0009929

Revision D.0

Oxoid Limited | Wade Road | Basingstoke, Hampshire UK RG24 8PW

For descriptions of symbols on product labels or product documents, go to thermoﬁsher.com/symbols-deﬁnition.

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE

LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR

ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Revision history: Pub. No. MAN0009929

Revision Date Description

D.0 8 July 2021 •Added ISO certiﬁcation

•Added QuantStudio™ 5 Real‑Time PCR Instrument with Thermo

Scientiﬁc™ RapidFinder™ Analysis Software v1.2 or later

C.0 7 August 2020 Updated the template. Minor edits throughout.

B.0 March 2015 •Updated speciﬁcity table

•Corrected volume of Positive Control

•Corrected quencher to NFQ-MGB

A.0 March 2014 New document

Trademarks: All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed. TaqMan is a

registered trademark of Roche Molecular Systems, Inc., used under permission and license. Imegen agro is a trademark of Instituto de

Medicina Genómica SL.

Contents

■CHAPTER 1 Product information .................................................. 5

Product description ............................................................. 5

Kit contents and storage ......................................................... 5

Materials required but not provided ................................................ 6

■CHAPTER 2 Methods ............................................................... 7

Input DNA requirements ......................................................... 7

Determine the number of reactions, then thaw the reagents .......................... 7

Set up the PCR reactions ........................................................ 7

Set up and run the real-time PCR instrument ....................................... 8

Analyze results .................................................................. 8

Interpretation of results .......................................................... 9

Quantitative analysis ............................................................. 9

■APPENDIX A Troubleshooting .................................................... 11

■APPENDIX B Supplemental information ........................................ 13

Kit sensitivity and speciﬁcity ..................................................... 13

UNE‑EN ISO 9001 certiﬁcation ................................................... 14

UNE‑EN ISO 14001 certiﬁcation ................................................. 14

■APPENDIX C Good laboratory practices for PCR .............................. 15

Plate layout suggestions ........................................................ 15

■APPENDIX D Safety ............................................................... 16

Chemical safety ................................................................ 17

Biological hazard safety ......................................................... 18

RapidFinder™ Poultry ID Kit User Guide 3

■APPENDIX E Documentation and support ...................................... 19

Food Safety support ............................................................ 19

Customer and technical support ................................................. 19

Related documentation ......................................................... 20

Limited product warranty ........................................................ 20

Contents

4RapidFinder™ Poultry ID Kit User Guide

Product information

Product description

Identiﬁcation of meat species present in food samples is an essential step for veriﬁcation of origin

and traceability of raw materials, as well as for quality control of handling and cleaning processes in

production lines. The Thermo Scientiﬁc™ RapidFinder™ Poultry ID Kit enables real-time PCR detection

of poultry DNA that is present in food and feed samples. The kit detects blends containing ≥0.01% of

poultry DNA.

The kit includes:

•All reagents necessary for the real‐time PCR reaction—speciﬁc FAM™-labeled probe and primers

for poultry mitochondrial DNA, DNA polymerase enzyme, and other buer components.

•An internal positive control (IPC)—VIC™-labeled probe, primers, and template, to monitor for PCR

inhibition.

•A Positive Control, to conﬁrm poultry DNA detection.

Unknown samples and control samples are provided by the investigator.

Kit contents and storage

Table 1 RapidFinder™ Poultry ID Kit (Cat. No. A24397)

Component Amount (48 reactions) Storage[1]

Poultry Master Mix (yellow pad) 360 µL –20°C

General Master Mix (white pad) 600 µL 4°C

Positive Control (yellow cap) 60 µL –20°C

[1] See the expiration date on the box.

RapidFinder™ Poultry ID Kit User Guide 5

Materials required but not provided

Unless otherwise indicated, all materials are available through the Thermo Fisher Microbiology ordering

process. MLS: Fisher Scientiﬁc (ﬁsherscientiﬁc.com) or other major laboratory supplier.

Catalog numbers that appear as links open the web pages for those products.

Item Source

Real-time PCR instrument, one of the following:

Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR System

RapidFinder™ Analysis Software v1.2 or later

Contact your local microbiology

sales representative.

Applied Biosystems™ 7500 Fast Real-Time PCR System

RapidFinder™ Express Software v.2.0 or later

Equipment

Adjustable micropipettors (10 µL, 20 µL, 200 µL) Available through the Thermo

Fisher Microbiology ordering

process. See thermoﬁsher.com/

plastics for more information.

Benchtop microcentrifuge with adaptors for PCR plates and/or tubes

Laboratory mixer (Vortex mixer or equivalent)

Optical reaction plates and covers, or optical PCR tubes and caps

MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL 4346907

MicroAmp™ Optical Adhesive Film, 100 covers 4311971

MicroAmp™ Fast 8-Tube Strip, 0.1 mL

(See below for caps.)

4358293

MicroAmp™ Optical 8-Cap Strips 4323032

Other plastics and consumables

Aerosol-resistant pipette tips Available through the Thermo

Fisher Microbiology ordering

process. See thermoﬁsher.com/

plastics for more information.

1.5-mL nuclease-free microcentrifuge tubes

Powder-free disposable gloves

Reagents

Nuclease-free water (not DEPC-Treated) AM9938

Recommended kits for DNA isolation, one of the following:

GMO Extraction Kit 4466336

For high-throughput isolation:

Lysis Buer 1 + RNase for Food ID

PrepSEQ™ Nucleic Acid Extraction Kit

A24401

4428176, 4480466

Chapter 1 Product information

Materials required but not provided

6RapidFinder™ Poultry ID Kit User Guide

Methods

Input DNA requirements

•Prepare the DNA sample with a method that allows processing of 10–20 g of food sample.

–For low-throughput, manual processing, use the GMO Extraction Kit.

–For high-throughput, automated processing, use Lysis Buer 1 + RNase for Food ID and the

PrepSEQ™ Nucleic Acid Extraction Kit with the KingFisher™ mL Magnetic Particle Processor.

•Prepare at least one mock-puriﬁed sample as a negative extraction control, processed with the

same DNA isolation method that is used for test samples.

•Dilute the ﬁnal DNA sample to 10 ng/µL for the PCR.

Determine the number of reactions, then thaw the reagents

1. Plan to include the following reactions:

•Single reactions for each test sample.

•Single control reactions.

–Positive Control (included in the kit).

–Negative extraction control (mock-puriﬁed samples).

–No-template control reactions; use nuclease-free water in place of sample DNA.

2. Thaw all reagents, vortex to mix thoroughly, then place on ice.

Set up the PCR reactions

1. Combine the following components for the number of reactions required plus 10% overage.

Component Volume per reaction

Poultry Master Mix (yellow pad) 7.5 µL

General Master Mix (white pad) 12.5 µL

2. Mix thoroughly by vortexing, then distribute 20 µL to each reaction well or tube.

3. Add 5 µL of DNA sample (10 ng/µL), mock-puriﬁed sample (negative extraction control),

nuclease-free water (no-template control), or Positive Control to the appropriate wells.

4. Seal each plate or tube, mix, then centrifuge brieﬂy to bring the contents to the bottom.

RapidFinder™ Poultry ID Kit User Guide 7

Set up and run the real-time PCR instrument

See the appropriate instrument user guide for detailed instructions to set up and run the real-time PCR

instrument.

1. Set up the real-time PCR instrument using the following settings:

•Reaction volume: 25 µL

•Passive reference dye: ROX™ dye included

•TaqMan™ probe reporter dyes and quenchers:

Target Reporter Quencher

Poultry DNA FAM™ dye NFQ-MGB

IPC VIC™ dye NFQ-MGB

•Thermal cycler settings:

Setting Stage 1

Enzyme activation

Stage 2

PCR

Number of cycles 1 (Hold) 36

Denature Anneal/extend[1]

Temperature 95°C 95°C 60°C

Time 10 minutes 15 seconds 1 minute

[1] Fluorescence is acquired during the annealing/extension stage.

2. Load the reactions, run the thermal cycler program and collect real-time ampliﬁcation data.

Analyze results

The general process for analyzing results is described in this section. The details of data analysis

depend on the real-time PCR instrument that you use; see the appropriate user guide for instructions

on how to analyze your data.

1. View the ampliﬁcation plots for all reactions to make sure that they appear normal.

2. Use the Auto instrument setting to set the baseline.

3. Set the FAM™ and VIC™ threshold to 0.1.

Chapter 2 Methods

Set up and run the real-time PCR instrument

8RapidFinder™ Poultry ID Kit User Guide

4. Check that the results obtained in all control wells are as expected. For unexpected control results,

see Appendix A, “Troubleshooting”.

Reaction type FAM™ channel

(Poultry DNA)

VIC™ channel

(IPC)[1]

Positive Control + +

Negative extraction control — +

No-template control — +

[1] In all reactions, the Ct of the IPC should be similar to the Ct of the Positive Control.

5. Establish the positive cut-o value for the test samples and assign results:

Ct (cut-o) = Ct (Positive Control) + 3.32

Sample Ct value Sample result

Ct > Ct (cut-o) Negative

Ct ≤ Ct (cut-o) Positive[1]

[1] For fresh or minimally processed meat samples, the cut-off value corresponds to approximately 0.01% poultry DNA, when the

DNA sample concentration is 10 ng/µL.

Interpretation of results

Interpret unknown sample results according to the following table.

FAM™ channel

(Poultry DNA)

VIC™ channel

(IPC)

Interpretation

— + Poultry DNA not detected.

+ + Poultry DNA detected.

— — Invalid result. See Appendix A, “Troubleshooting”.

+ — This result is expected in reactions that have

a strong FAM™ signal. See Appendix A,

“Troubleshooting”.

Quantitative analysis

For samples with positive results, the percentage of poultry DNA with respect to total animal DNA

can be quantiﬁed using the RapidFinder™ Poultry ID Kit in combination with the RapidFinder™ Quant

Multi-Meat Set (Cat. No. A24399).

The RapidFinder™ Quant Multi-Meat Set includes the Multi-Meat Standard, a plasmid DNA quantitation

standard containing individual species-speciﬁc DNA targets and a highly conserved animal-speciﬁc

mitochondrial genomic region (total animal DNA target). The kit also includes a TaqMan™ assay for the

total animal DNA target. Both species-speciﬁc and total animal DNA present in each sample can be

Chapter 2 Methods

Interpretation of results 2

RapidFinder™ Poultry ID Kit User Guide 9

quantiﬁed relative to the Multi-Meat Standard using primer and probe sets from both kits. For detailed

instructions, see the RapidFinder™ Quant Multi-Meat Set User Guide (Pub. No. MAN0009930).

Note: The Positive Control included in the RapidFinder™ Poultry ID Kit is not intended for use as a

quantitation standard.

Chapter 2 Methods

Quantitative analysis

10 RapidFinder™ Poultry ID Kit User Guide

Troubleshooting

Observation Possible cause Recommended action

In the Positive Control wells,

no target-speciﬁc and no IPC

signals are detected.

PCR ampliﬁcation failure. Check that the thermal cycler settings and

ampliﬁcation program are correct.

In the negative extraction

control wells, target-speciﬁc

and IPC signals are detected.

Contamination during the DNA

extraction procedure.

Contamination may be due to errors in

sample handling, reagent contamination, or

environmental contamination.

•Check that the DNA extraction protocol

was performed correctly.

•Take care to avoid contamination during

sample homogenization: decontaminate

grinding equipment or homogenizer with

10% bleach or DNAZap™ Solutions (Cat.

No. AM9890).

•Decontaminate benchtop surfaces and

other equipment where the DNA

extraction process is performed with

10% bleach or DNAZap™ Solutions.

•If necessary, use fresh reagents and

repeat the DNA extraction.

In the no-template control

wells, target-speciﬁc and IPC

signals are detected.

Contamination of the PCR. Contamination may be due to errors in

sample handling, reagent contamination, or

environmental contamination.

•Decontaminate benchtop surfaces and

other equipment where PCR is performed

with 10% bleach or DNAZap™ Solutions

(Cat. No. AM9890).

•Use fresh reagents and repeat the PCR.

•Set up the Positive Control PCR reactions

last to avoid cross-contamination.

In unknown wells, no IPC

signal is detected, but target-

speciﬁc signal is detected.

A high copy number of

target DNA exists in samples,

resulting in preferential

ampliﬁcation of the target-

speciﬁc DNA.

No action is required. The result is considered

positive.

In unknown wells, no IPC

or target-speciﬁc signal is

detected.

Excess sample DNA in

the PCR; the recommended

maximum is 250 ng.

Repeat the PCR with the correct amount of

DNA. If DNA quantiﬁcation is not possible,

dilute the DNA sample.

RapidFinder™ Poultry ID Kit User Guide 11

Observation Possible cause Recommended action

In unknown wells, no IPC

or target-speciﬁc signal is

detected.

(continued)

PCR inhibitors in the sample

DNA.

Repeat the DNA extraction. If the problem

persists, contact Technical Support.

Appendix A Troubleshooting

Quantitative analysis

12 RapidFinder™ Poultry ID Kit User Guide

Supplemental information

Kit sensitivity and speciﬁcity

The detection limit was calculated with standard samples consisting of mixtures of raw poultry meat

and other species. The RapidFinder™ Poultry ID Kit can detect blends with as little as 0.01% (w/w) of

poultry meat. The limit of detection in processed samples varies depending upon the composition and

food processing.

The kit speciﬁcity was tested by comparison of the probe and primer sequences with the NCBI

database, and it was also experimentally tested on a collection of reference DNAs, with the following

results.

Note: The primers and probes in this kit have been designed to detect any kind of bird species. It is

more common to detect poultry meat in food samples, but it is possible to use this kit to detect bird

species DNA in general.

Meat species Result

Sheep Not detected

Goat Not detected

Horse Not detected

Beef Not detected

Pork Not detected

Bualo Not detected

Deer Not detected

Chicken Detected

Turkey Detected

Duck Detected

Ostrich Detected

Goose Detected

Human Not detected

Fish Not detected

RapidFinder™ Poultry ID Kit User Guide 13

UNE‑EN ISO 9001 certiﬁcation

Imegen is certiﬁed against the standard UNE-EN ISO 9001:2015 "Quality management systems" for the

design, development, manufacture, and commercialization of kits for genetic analysis.

UNE‑EN ISO 14001 certiﬁcation

Imegen is certiﬁed against the standard UNE-EN ISO 14001:2015 “Environmental Management

Systems” for the design, development, manufacture, and commercialization of kits for genetic analysis.

Appendix B Supplemental information

UNE‑EN ISO 9001 certiﬁcation

14 RapidFinder™ Poultry ID Kit User Guide

Good laboratory practices for PCR

To avoid amplicon contamination of samples, follow these guidelines when preparing or handling

samples for PCR ampliﬁcation:

•Wear clean gloves and a clean lab coat (not previously worn while handling ampliﬁed products or

used during sample preparation).

•Change gloves whenever you suspect that they are contaminated.

•Maintain separate areas and dedicated equipment and supplies for:

–Sample preparation and reaction setup.

–Ampliﬁcation and analysis of products.

•Do not bring ampliﬁed products into the reaction setup area.

•Open and close all sample tubes carefully. Avoid splashing or spraying samples.

•Keep reactions and components capped as much as possible.

•Use a positive-displacement pipettor or aerosol-resistant barrier pipette tips.

•Do not open reaction tubes after PCR.

•Do not autoclave reaction tubes after PCR.

•Clean lab benches and equipment periodically with 10% bleach solution or DNAZap™ Solutions

(Cat. No. AM9890). After cleaning with bleach we recommend a rinse with an ethanol solution

because bleach will rust stainless steel.

For additional information, refer to EN ISO 22174:2005 or www.thermoﬁsher.com/us/en/home/life-

science/pcr/real-time-learning-center/real-time-pcr-basics.html.

Plate layout suggestions

•Separate dierent targets by a row if enough space is available.

•Put at least one well between unknown samples and controls if possible.

•Separate negative and positive controls by one well if possible.

•Place replicates of one sample for the same target next to each other.

•Start with the unknown samples and put controls at the end of the row or column.

•Put positive controls in one of the outer rows or columns if possible.

•Consider that caps for PCR tubes come in strips of 8 or 12.

RapidFinder™ Poultry ID Kit User Guide 15

Safety

WARNING! GENERAL SAFETY. Using this product in a manner not speciﬁed in the user

documentation may result in personal injury or damage to the instrument or device. Ensure that

anyone using this product has received instructions in general safety practices for laboratories and

the safety information provided in this document.

·Before using an instrument or device, read and understand the safety information provided in the

user documentation provided by the manufacturer of the instrument or device.

·Before handling chemicals, read and understand all applicable Safety Data Sheets (SDSs) and use

appropriate personal protective equipment (gloves, gowns, eye protection, and so on). To obtain

SDSs, see the “Documentation and Support” section in this document.

16 RapidFinder™ Poultry ID Kit User Guide

Chemical safety

WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel

read and practice the general safety guidelines for chemical usage, storage, and waste provided

below. Consult the relevant SDS for speciﬁc precautions and instructions:

·Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer

before you store, handle, or work with any chemicals or hazardous materials. To obtain SDSs, see

the "Documentation and Support" section in this document.

·Minimize contact with chemicals. Wear appropriate personal protective equipment when handling

chemicals (for example, safety glasses, gloves, or protective clothing).

·Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with

sucient ventilation (for example, fume hood).

·Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer

cleanup procedures as recommended in the SDS.

·Handle chemical wastes in a fume hood.

·Ensure use of primary and secondary waste containers. (A primary waste container holds the

immediate waste. A secondary container contains spills or leaks from the primary container.

Both containers must be compatible with the waste material and meet federal, state, and local

requirements for container storage.)

·After emptying a waste container, seal it with the cap provided.

·Characterize (by analysis if needed) the waste generated by the particular applications, reagents,

and substrates used in your laboratory.

·Ensure that the waste is stored, transferred, transported, and disposed of according to all local,

state/provincial, and/or national regulations.

·IMPORTANT! Radioactive or biohazardous materials may require special handling, and disposal

limitations may apply.

AVERTISSEMENT ! PRÉCAUTIONS GÉNÉRALES EN CAS DE MANIPULATION DE PRODUITS

CHIMIQUES. Pour minimiser les risques, veiller à ce que le personnel du laboratoire lise attentive‐

ment et mette en œuvre les consignes de sécurité générales relatives à l’utilisation et au stockage

des produits chimiques et à la gestion des déchets qui en découlent, décrites ci-dessous. Consulter

également la FDS appropriée pour connaître les précautions et instructions particulières à respecter :

·Lire et comprendre les ﬁches de données de sécurité (FDS) fournies par le fabricant avant de

stocker, de manipuler ou d’utiliser les matériaux dangereux ou les produits chimiques. Pour obtenir

les FDS, se reporter à la section « Documentation et support » du présent document.

·Limiter les contacts avec les produits chimiques. Porter des équipements de protection appropriés

lors de la manipulation des produits chimiques (par exemple : lunettes de sûreté, gants ou vête‐

ments de protection).

·Limiter l’inhalation des produits chimiques. Ne pas laisser les récipients de produits chimiques

ouverts. Ils ne doivent être utilisés qu’avec une ventilation adéquate (par exemple, sorbonne).

·Vériﬁer régulièrement l’absence de fuite ou d’écoulement des produits chimiques. En cas de fuite

ou d’écoulement d’un produit, respecter les directives de nettoyage du fabricant recommandées

dans la FDS.

·Manipuler les déchets chimiques dans une sorbonne.

Appendix D Safety

Chemical safety D

RapidFinder™ Poultry ID Kit User Guide 17

·Veiller à utiliser des récipients à déchets primaire et secondaire. (Le récipient primaire contient les

déchets immédiats, le récipient secondaire contient les fuites et les écoulements du récipient pri‐

maire. Les deux récipients doivent être compatibles avec les matériaux mis au rebut et conformes

aux exigences locales, nationales et communautaires en matière de conﬁnement des récipients.)

·Une fois le récipient à déchets vidé, il doit être refermé hermétiquement avec le couvercle fourni.

·Caractériser (par une analyse si nécessaire) les déchets générés par les applications, les réactifs et

les substrats particuliers utilisés dans le laboratoire.

·Vériﬁer que les déchets sont convenablement stockés, transférés, transportés et éliminés en res‐

pectant toutes les réglementations locales, nationales et/ou communautaires en vigueur.

·IMPORTANT ! Les matériaux représentant un danger biologique ou radioactif exigent parfois une

manipulation spéciale, et des limitations peuvent s’appliquer à leur élimination.

WARNING! HAZARDOUS WASTE (from instruments). Waste produced by the instrument is

potentially hazardous. Follow the guidelines noted in the preceding General Chemical Handling

warning.

WARNING! 4L Reagent and Waste Bottle Safety. Four-liter reagent and waste bottles can crack

and leak. Each 4-liter bottle should be secured in a low-density polyethylene safety container with the

cover fastened and the handles locked in the upright position.

Biological hazard safety

WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface

may be considered a biohazard. Use appropriate decontamination methods when working with

biohazards.

WARNING! BIOHAZARD. Biological samples such as tissues, body ﬂuids, infectious agents,

and blood of humans and other animals have the potential to transmit infectious diseases.

Conduct all work in properly equipped facilities with the appropriate safety equipment (for example,

physical containment devices). Safety equipment can also include items for personal protection,

such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or

goggles. Individuals should be trained according to applicable regulatory and company/ institution

requirements before working with potentially biohazardous materials. Follow all applicable local,

state/provincial, and/or national regulations. The following references provide general guidelines when

handling biological samples in laboratory environment.

·U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical

Laboratories (BMBL), 6th Edition, HHS Publication No. (CDC) 300859, Revised June 2020

https://www.cdc.gov/labs/pdf/CDC-BiosafetymicrobiologicalBiomedicalLaboratories-2020-

P.pdf

·Laboratory biosafety manual, fourth edition. Geneva: World Health Organization; 2020 (Laboratory

biosafety manual, fourth edition and associated monographs)

www.who.int/publications/i/item/9789240011311

Appendix D Safety

Biological hazard safety

18 RapidFinder™ Poultry ID Kit User Guide

Documentation and support

Food Safety support

Website: thermoscientiﬁc.com/foodmicro or thermoﬁsher.com/foodsafety

Imegen website for Certiﬁcates of Analysis and other product documentation: https://

portal.imegen.es/en/certiﬁcate-of-analysis/

Support email:

•Europe, Middle East, Africa: microbiology.techsupport.uk@thermoﬁsher.com

•North America: microbiology@thermoﬁsher.com

Phone: Visit thermoﬁsher.com/support, select the link for phone support, then select the appropriate

country from the dropdown list.

Customer and technical support

Visit thermoﬁsher.com/support for the latest service and support information.

•Worldwide contact telephone numbers

•Product support information

–Product FAQs

–Software, patches, and updates

–Training for many applications and instruments

•Order and web support

•Product documentation

–User guides, manuals, and protocols

–Certiﬁcates of Analysis

–Safety Data Sheets (SDSs; also known as MSDSs)

Note: For SDSs for reagents and chemicals from other manufacturers, contact the

manufacturer.

RapidFinder™ Poultry ID Kit User Guide 19

Thermo Fisher Scientific RapidFinder Poultry ID Kit Mode d'emploi

Marque: Thermo Fisher Scientific

Modèle: RapidFinder Poultry ID Kit

Taper: Mode d'emploi

Télécharger le PDF

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Thermo Fisher Scientific RapidFinder Poultry ID Kit Mode d'emploi

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