Mettler Toledo InPro 5000 (i) CO2 Sensors Mode d'emploi

Taper
Mode d'emploi
InPro 5000 (i) CO2 Sensors
Instruction manual
Bedienungsanleitung
Instructions d’utilisation
InPro 5000 (i)
52 002 465 F
INGOLD
Leading Process Analytics
2 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
English Page 3
Deutsch Seite 21
Français Page 39
InPro 5000 (i) CO2 Sensor 12 mm 3
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
InPro 5000 (i) CO2 Sensors
Instruction manual
InPro, InFit, ISM and InTrac are trademarks
of the METTLER TOLEDO Group.
4 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
1 Introduction ....................................................... 5
2 Important notes ................................................. 6
2.1 Notes on operating instructions ............................ 6
2.2 Intended use ...................................................... 6
2.3 Safety instructions ............................................... 7
2.4 Environmental protection ..................................... 7
3 Product description ............................................ 8
3.1 General information ............................................. 8
3.2 Principles of CO2 measurement ............................ 8
3.3 Scope of delivery ................................................ 8
3.4 Equipment features ............................................. 9
4 Preparation ..................................................... 10
4.1 Start-up ........................................................... 10
4.2 Calibration ....................................................... 11
4.2.1 Connecting the InPro 5000 (i) to cable ............... 11
4.2.2 Connecting the cable to the transmitter ................ 12
4.2.3 Calibation of pH electrode .................................. 12
4.3 Mounting the membrane body ............................ 13
4.4 Mounting the sensor .......................................... 14
4.5 Sterilization ...................................................... 14
5 Operation ........................................................ 15
5.1 Calibration of the CO2 sensor .............................. 15
6 Maintenance ................................................... 16
6.1 Inspection ........................................................ 16
6.1.1 Visual inspection .............................................. 16
6.1.2 Checking wiring and transmitter
with the pH simulator ........................................ 16
6.2 Changing the CO2 electrolyte
and membrane body ......................................... 16
6.3 Maintenance of the interior body (pH electrode) .... 17
6.4 Replacement of the interior body (pH electrode) ... 17
6.5 ISM ................................................................ 17
7 Storage ........................................................... 18
8 Productspecication ....................................... 18
8.1 Certicates ....................................................... 18
8.2 Specications ................................................... 18
9 Ordering information ........................................ 19
9.1 Sensors ........................................................... 19
9.2 Accessories ...................................................... 19
9.3 Spare parts ...................................................... 19
9.4 Transmitter ....................................................... 20
Contents
InPro 5000 (i) CO2 Sensor 12 mm 5
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
1 Introduction
Thank you for buying the InPro™ 5000 (i) sensor
from METTLER TOLEDO.
The construction of the InPro 5000 (i) sensor employs
leading edge technology and complies with safety
regulations currently in force. Notwithstanding this,
improper use could lead to hazards for the user or
a third-party, and / or adverse effects on the plant
or other equipment.
a
Therefore, the operating instructions must be read
and understood by the persons involved before work
is started with the sensor.
The instruction manual must always be stored close at
hand, in a place accessible to all people working with
the InPro 5000 (i).
Ifyouhavequestions,whicharenotorinsufciently
answered in this instruction manual, please contact
your METTLER TOLEDO supplier. They will be glad to
assist you.
6 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
2 Important notes
2.1 Notes on operating instructions
These operating instructions contain all the information
needed for safe and proper use of the InPro 5000 (i)
sensor.
The operating instructions are intended for personnel
entrusted with the operation and maintenance of the
sensors. It is assumed that these persons are familiar
with the equipment in which the sensor is installed.
Warning notices and symbols
This instruction manual identies safety instructions
and additional information by means of the following
symbols:
a
This symbol draws attention to safety instructions
and warnings of potential danger which, if neglec-
ted, could result in injury to persons and / or damage
to property.
h
This symbol identies additional information and
instructions which, if neglected, could lead to defects,
inefcientoperationandpossiblelossofproduction.
2.2 Intended use
InPro 5000 (i) sensors are intended solely for in
line measurement of dissolved CO2 (carbon diox-
ide), as described in this instruction manual.
Any other use, or use not mentioned here, that is in-
compatiblewiththetechnicalspecicationsisdeemed
inappropriate. The operator is solely responsible for
any damage arising from such use.
Other prerequisites for appropriate use include:
observing the instructions, notes and requirements
set out in this instruction manual.
observing all local safety regulations concerning
safety on work.
observing all information and warnings in the
documentation dealing with the products used
togeth er with the sensor (housings, transmitters,
etc.).
observing all safety regulations governing
the equipment in which the sensor is installed.
observing the prescribed inspection and mainte-
nance intervals.
correct care and maintenance of the unit,
according to the instruction manual.
observing the prescribed environmental and
operational conditions, and permitted installation
positions.
InPro 5000 (i) CO2 Sensor 12 mm 7
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
2.3 Safety instructions
The InPro 5000 (i) sensors should be installed,
operated, maintained only by personnel familiar
with the sensor and who are qualied for such
work.
A defective sensor must neither be install ed nor put
into service.
Only the maintenance work described in these
operating instructions may be performed on the
sensors.
When changing faulty components, use only
original spare parts obtainable from your METTLER
TOLEDO supplier (see section 9).
No modications to the sensors and the
accessories are allowed. The manufacturer/
supplier accepts no responsibility for damage
causedbyunauthorisedmodications.Theriskis
borne entirely by the user.
2.4 Environmental protection
Waste electrical products should not be disposed of
with household waste. Please recycle where facilities
exist. Check with your Local Authority or retailer for
recycling advice.
a
8 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
3 Product description
3.1 General information
The sensor InPro 5000 (i) with integrated temperature
probe is used for dissolved carbon dioxide meas ure-
ment.
The sensor has been designed for accurate and precise
measurement at low to medium carbon dioxide partial
pressure.
The sensor is sterilizable and compatible with CIP
(cleaning in place).
The sensor consists of an exchangeable pH electrode
whichisbuiltinasteelshaftandxedwithasmall
hexagonal screw.
3.2 Principles of CO2 measurement
Here is a short summary of the principle of the
potentiometric measurement of CO2 which this
sensor is based (Severinghaus). The InPro 5000 (i)
sensor employs a gas permeable silicone membrane
which is tightly stretched around a special engineered
atpHmembrane.TheCO2 gas from the sample or
process diffuses across the membrane until its partial
pressureequilibrateswithintheelectrolytelminfront
of the pH sensitive glass and the bulk of the internal
electrolyte.
The dissolved CO2 gas reacts with water to form
bicarbonate and H+ ions:
HCO3
+ H+
CO2
+
H2O
The formed H+ ions lead to a pH change of the in-
ner electrolyte which is measured with the integrated
pH electrode. Thus, the CO2 partial pressure can be
calculated using the pH and the temperature, which is
detected as well.
3.3 Scope of delivery
Each sensor is supplied together with:
1 bottle CO2-electrolyte 25 ml
1qualitycontrolcerticate
1inspectioncerticate3.1
(in accordance with EN 10204.3/3.1)
Membrane body and cap sleeve are not mounted.
The interior body is protected against drying out with a
wettingcap,lledwithCO2-electrolyte.
Please check completeness.
InPro 5000 (i) CO2 Sensor 12 mm 9
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
3.4 Equipment features
VP connector
O-ring (10.77 x 2.62 mm,
silicone FDA)
Pg 13.5 threaded sleeve
Washer PTFE
Cap sleeve
Membrane body
Wetting cap
Interior body (pH electrode)
Hexagonal screw
ISM connector
O-ring (10.77 x 2.62 mm,
silicone FDA)
Pg 13.5 threaded sleeve
Washer PTFE
Cap sleeve
Membrane body
Wetting cap
Interior body (pH electrode)
Hexagonal screw
Retainer nut
InPro 5000 i
InPro 5000
10 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
4 Preparation
4.1 Start-up
The interior body (pH electrode) is ready for calibration.
On unpacking, check the pH electrode for
mechanical damage. Report any damage imme-di-
ately to your METTLER TOLEDO supplier.
Remove the watering cap, cap sleeve, membrane
bodyandbrieyrinsethesensorwithde-ionized
water.
After rinsing, the sensor should only be dapped dry
with a tissue. Do not rub the pH sensitive glass,
since this can lead to electrostatic charging and
sluggish response times.
Check inside the pH sensitive glass for the pres-
ence of any air bubbles and remove same by gen-
tly swinging in a vertical plane (shaking down as
with a thermometer).
Beforeusingthesensorforthersttime,makesurethat
theinteriorbody(pHelectrode)iswellxedintothesteel
body. The VP connector must not be rotated in the shaft.
Ifso,theinnerparthastobexedbytighteningthesmall
hexagonal screw below the connector.
A: pH electrode
with air bubbles
will not work.
B: Correct pH electrode
with removed bubbles.
pH sensitive
glass
Ceramic
diaphragm
AB
Air
bubbles
InPro 5000 (i) CO2 Sensor 12 mm 11
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
4.2 Calibration
Purpose of calibration: Each CO2 sensor and Interior
body (pH electrode) has its individual slope and zero
point.
4.2.1 Connecting the InPro 5000 (i) to cable
The sensor is connected to the transmitter via a VP or
AK9 cable ensuring a secure connection under harsh
industrial conditions. The robust watertight IP 68 con-
nector housing guarantees maximum process safety.
To connect the VP cable to the sensor align the notch of
the VP connector with the key in the plug. Then tightly
screw the plug to fasten the two parts.
VP cable
Plug
Pin
Notch
VP connector
AK9 cable
Plug
Pin
Notch
ISM connector
InPro 5000 InPro 5000 i
12 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
4.2.2 Connecting the cable to the transmitter
For connecting the sensor please refer to the manual
of the transmitter.
4.2.3 Calibation of pH electrode
(Not needed for ISM digital InPro 5000 i)
Prepare electrode as described in section 4.1 “Start-
up”. For the analog CO2 sensor InPro 5000, we re-
commend a 2-point calibration in pH buffer pH
7.00 and pH 9.21. Before calibration, rst remove
wetting cap. Normally you start with buffer pH 7.00
for determining zero point and then buffer pH 9.21
for determining the slope. Please refer to instruction
manual of the transmitter for further details.
h
Important settings : On the transmitter, select Pt1000
as temperature sensor for the analog InPro 5000. ISM
digital InPro 5000 i does not require a temperature set-
ting.
h Important: Please do not leave the sensor in the cali-
bration buffer for longer than 1 hour. Too long exposure
may result in a high sensor drift in the process.
VP Cable
CO2 Transmitter
AK9 Cable
Min. immersion depth
> 25 >mm / 1"
InPro 5000 (i) CO2 Sensor 12 mm 13
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
4.3 Mounting the membrane body
After calibration of the pH electrode rinse the pH
electrode with CO2 electrolyte solution.
Fill the new membrane body half way with CO2
electrolyte.
Make sure that all air bubbles are removed from the
membrane body. Air bubbles can be removed by care-
fully tapping on the membrane body.
Slip the membrane body over the pH electrode
while holding the sensor in a vertical position.
The excess electrolyte will be displaced should
be wiped dry with a paper tissue.
h
Important: No electrolyte, sample media or
contamination may be present between the
membrane body and the cap sleeve. Please check
carefully.
Carefully slip the cap sleeve over the tted
membrane body, holding the sensor in a vertical
position and screw it tight. The cap sleeve must be
clean and tight.
1/2
1/2CO2 electrolyte
14 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
4.4 Mounting the sensor
Please read carefully the instruction manual of the
particular housing (InFit761-... or InTrac797) for
correct sensor installation.
The InPro 5000 (i) sensor can be mounted directly on
a top plate of a small bioreactor or in a pipe with an
inside Pg 13.5 thread and securely tightened via the
Pg 13.5 threaded sleeve.
h
Remark: Mounting upside-down or horizontaly is not
possible due to internal pH electrode. An angle of 15°
or bigger to horizontal is needed.
4.5 Sterilization
The installed sensor will be sterilized together with the
bioreactor. The InPro5000 is in-situ sterilizable or in an
autoclave. After cooling down but before inoculation a
process calibration step, as described in chapter 5, is
needed to achieve accurate measurements.
h
Remark: For best accuracy please wait one hour after
cooling down before process calibration step accor-
ding chapter 5.1.
InPro 5000 (i) CO2 Sensor 12 mm 15
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
5 Operation
5.1 Calibration of the CO2 sensor
The InPro 5000 (i) sensor can be calibrated with CO2
gas or a CO2 / N2 gas mixture. Example: If a CO2
partial pressure of 150 mbar is expected, you can use
a gas with 15 % CO2/ 85 % N2 in nitrogen to achieve
best results. The calibration can be made in a gas or
liquid phase. For calibration in a solution you have to
wait until an equilibrium is reached. Depending on the
pH value of the solution a large amount of CO2 will
react to build bicarbonate ions before building-up a
constant partial pressure of CO2.
h
Recommendations:
For small “benchtop” bioreactors the sensor can
be calibrated by ushing the empty or liquid
lledreactorwithaCO2 containing gas mixture.
For larger bioreactors the gas consumption would
be too high and therefore we recommend the
calibration with the aid of the retractable
housing InTrac 797. Please operate the retract-
able housing according the instruction manual
InTrac 797 to ensure sterile operation. Additionally
thein- and outlet ofthe lower ushing chamber
mustbeequippedwithsterilelterstoavoidany
contamination with the calibration gas.
Calibration procedure:
Sterilize CO2 sensor InPro 5000 (i) in measuring
position (membrane inside bioreactor) together
with the bioreactor.
Sterilize lower and upper ushing chambers
of housing InTrac 797.
After cooling down retract sensor in maintenance
position (membrane in lower chamber).
Flush lower chamber with sterile calibration gas.
Thepressureinsidetheushingchambermustbe
known for proper calibration.
After calibration close in- and outlet of the
lower chamber and reinsert sensor in measuring
position. Sensor is now ready for measurement.
16 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
6 Maintenance
6.1 Inspection
6.1.1 Visual inspection
To check the sensor, we recommend the following pro-
cedure:
The contacts of the connector must be dry. Mois-
ture, corrosion and dirt can lead to false readings.
Check the cable for kinks, brittle areas or tears.
Examine the membrane foil optically for signs of
damage. The foil must be intact and clean. Dirty
membranes should be wip ed clean using a soft,
moist tissue.
h
Note:Anundulatedmembranehasnoinuenceonthe
sensor performance, assuming the membrane is intact.
The membrane body must be replaced if the sensor
has a long response time, the reading is unstable
or subject to excessive drift, and if the sensor can-
not be calibrated with CO2 gas or the membrane
shows sign of mechanical damage.
Check the pH sensitive glass for cracks. The dia-
phragm should show a white color. If necessary
rinse with deionized water and clean carefully with
soft paper tissue.
a
Attention! Do not use any cleaning agents containing
alcohol. This could damage the sensor or lead to
fault results.
a
Attention! The pH electrode is made of glass and
therefore fragile.
6.1.2 Checking wiring and transmitter
with the pH simulator
Necessary equipment for analog CO2 sensor
InPro 5000:
pH simulator 112 (p/n 59 906 431) and VP module
(p/n 52 120 939).
This equipment enables the testing of the VP cable
and correct wiring of the cable to the transmitter. Switch
the VP simulator to Pt 1000 position. Additionally it is
possible to check the linearity, temperature compen-
sation and quality of the input circuit (input resistance
and input current) of the CO2 transmitter.
6.2 Changing the CO2 electrolyte
and membrane body
We recommend to use a new membrane body after
each batch (see section 4.3). After each calibration
of the pH electrode (section 4.2) new CO2 electrolyte
shouldbelledinthemembranebody.
InPro 5000 (i) CO2 Sensor 12 mm 17
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
6.3 Maintenance of the interior body (pH electrode)
If the pH electrode shows a sluggish response
time and/or insufcient sensitivity the pH sensi-
tive glass can be reactivated using a HF solution
(p/n 51 319 053). The pH sensitive glass part only
is immersed into this solution for 5 minutes, is then
immediately and thoroughly rinsed with deionized
water and then stored in CO2 Electrolyte up to the di-
aphragm for 12 hours. This procedure will make the
electrode faster but will limit the life time hardly.
6.4 Replacement of the interior body (pH electrode)
Typically after 10 20 sterilization cycles the pH
electrode should be replaced. Please refer to the
instruction manual of the replacement interior body
InPro 5000 (i).
6.5 ISM
DLI: Dynamic Lifetime Indicator
The DLI provides information about the remaining lifeti-
me of the interior body. As long as the DLI is above zero
daysthesystemiswithinthespeciedaccuracyaftera
calibration. If the DLI is zero the inner body needs to be
replaced.
Contributing factors for ageing:
temperature during measurement
number of CIP cycles
number of SIP cycles
number of autoclavings
The DLI is calculated in the following way.
Continuously: With the above parameters an actual
sensor stress is calculated. During the operation the
sensor load is increased. The accumulated sensor load
divided by the elapsed time is the basis of the calcula-
tion of the remaining lifetime.
TTM: Time to Maintenance
The TTM gives an indication when maintenance needs
to be performed. Maintenance includes
1. Electrolyte exchange
2. Cleanig of the inner body
3. Replacement of the membrane body
ACT: Adaptive Calibration Timer
The ACT provides information as to when the next
calibration is required to ensure measurements will
remainwithinthespeciedaccuracy.Thiscalculation
is based on the DLI information.
Calibration history
The last four calibrations and the factory calibration
data are stored in the sensor memory. These data can
be read out with a transmitter or with the iSense Asset
Suite software.
The calibration history gives valuable information re-
garding the quality of the calibration and the ageing of
the interior body.
18 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
7 Storage
h
For storage we recommend to ll the membra-
ne body with fresh CO2 electrolyte and rebuilt the
sensor.Thewettingcap,lledwithCO2-electrolyte, will
prevent a drying-out of the membrane.
8 Productspecication
8.1 Certicates
Each sensor is delivered with a set of 3.1certicates
(complying with EN 10204/3.1).
All wetted metal parts (sensor shaft, cap sleeve and mem-
branebody)areidentiedwithanengraved symbol corre-
sponding to the heat number onthepapercerticate
delivered with the sensor.
Each wetted metal part (sensor shaft, cap sleeve and
membrane body) is polished in order to get a surface rough-
ness lower than 0.4 µm (16 µin). This represents a rough-
ness grade number of N5 (according to ISO 1320:1992).
8.2 Specications
InPro 5000 (i)
Measurement principle Potentiometric / Severinghaus
Working conditions
Mechanical pressure resistance max. 3 bar at 25 °C
[max. 42 psi at 77 °F]
Pressure range measurement 0.2 – 2 bar absolute
[3 – 30 psi absolute]
Temperature range
(measurement)
0…60 °C [32…140 °F]
Sterilization < 130 °C [266 °F] in situ
or autoclave
Construction
Temperature signal InPro 5000 i: Digital
InPro 5000: Pt 1000
Cable InPro 5000 i: AK9
InPro 5000: VP
O-ring material Viton®, Silicone (FDA approved)
Membrane material PTFE-silicone (reinforced)
Wetted metal parts Stainless steel DIN 1.4435
similar to AISI 316 L
Dimensions
Sensor diameter 12 mm (0.47”)
Immersion length 120, 220, 320 mm
(4.7, 8.66, 12.6”)
Performance
Measuring range 10 – 1000 hPa CO2
Accuracy ± 10 %, 600 – 900 hPa
≥±10%,>900hPa
Detection limit 10 hPa CO2
Response time 90%ofnalvalue<120sec
(at 25 °C [77 °F] from air to CO2)
InPro 5000 (i) CO2 Sensor 12 mm 19
© 03 / 2023 METTLER TOLEDO InPro 5000 (i)
Printed in Switzerland 52 002 465 G
9 Ordering information
9.1 Sensors
Designation:
InPro 5000 (i) / 12 / a (a = immersion length in mm)
Designation Order No.
InPro5000 i/12/120 30 013 606
InPro5000 i/12/220 30 019 005
InPro5000 i/12/320 30 019 006
InPro5000 i/12/420 30 748 118
9.2 Accessories
Accessory Order No.
VP cable VP6-ST/3 m 52 300 108
VP cable VP6-ST/5 m 52 300 109
VP cable VP6-ST/10 m 52 300 110
AK9 cable/1 m 59 902 167
AK9 cable/3 m 59 902 193
AK9 cable/5 m 59 902 213
AK9 cable/10 m 59 902 230
AK9 cable/20 m 52 300 204
ISMVericationKit 30 031 035
pH simulator 112 for InPro 5000 59 906 431
VP module for InPro 5000 52 120 939
9.3 Spare parts
Spare part Order No.
Membrane kit InPro 5000 52 206 055
Cap sleeve, N type 1.4435 52 201 153
Cap sleeve, P type 1.4435 52 201 154
Interior body InPro 5000 i/12/120 Kit 30 019 049
Interior body InPro 5000 i/12/220 Kit 30 019 170
Interior body InPro 5000 i/12/320 Kit 30 019 175
Interior body InPro 5000 i/12/420 Kit 30 749 397
pH buffer pH 7.00 250 ml 51 340 059
pH buffer pH 7.00 6 3 250 ml 51 340 060
pH buffer pH 9.21 250 ml 51 300 193
pH buffer pH 9.21 6 3 250 ml 51 300 194
Please check!
20 InPro 5000 (i) CO2 Sensor 12 mm
InPro 5000 (i) © 03 / 2023 METTLER TOLEDO
52 002 465 G Printed in Switzerland
9.4 Transmitter
Compatibility
InPro 5000 i InPro 5000
M400 Type 3 M400 Type 3
M800 M700 with CO2 module
Designation Order No.
M400 Type 3 52 121 350
M700 CO2 5700 i (analog only) 52 121 267
M800 Process 2-Ch 52 121 813
M800 Process 4-Ch 52 121 853
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Mettler Toledo InPro 5000 (i) CO2 Sensors Mode d'emploi

Taper
Mode d'emploi