Mettler Toledo InPro 5000 (i) CO2 Sensors Mode d'emploi

InPro 5000 (i) CO2 Sensors

Instruction manual

Bedienungsanleitung

Instructions d’utilisation

InPro 5000 (i)

52 002 465 F

INGOLD

Leading Process Analytics

2 InPro 5000 (i) CO2 Sensor 12 mm

InPro 5000 (i) © 03 / 2023 METTLER TOLEDO

52 002 465 G Printed in Switzerland

English Page 3

Deutsch Seite 21

Français Page 39

InPro 5000 (i) CO2 Sensor 12 mm 3

Printed in Switzerland 52 002 465 G

InPro 5000 (i) CO2 Sensors

Instruction manual

InPro, InFit, ISM and InTrac are trademarks

of the METTLER TOLEDO Group.

4 InPro 5000 (i) CO2 Sensor 12 mm

InPro 5000 (i) © 03 / 2023 METTLER TOLEDO

52 002 465 G Printed in Switzerland

1 Introduction ....................................................... 5

2 Important notes ................................................. 6

2.1 Notes on operating instructions ............................ 6

2.2 Intended use ...................................................... 6

2.3 Safety instructions ............................................... 7

2.4 Environmental protection ..................................... 7

3 Product description ............................................ 8

3.1 General information ............................................. 8

3.2 Principles of CO2 measurement ............................ 8

3.3 Scope of delivery ................................................ 8

3.4 Equipment features ............................................. 9

4 Preparation ..................................................... 10

4.1 Start-up ........................................................... 10

4.2 Calibration ....................................................... 11

4.2.1 Connecting the InPro 5000 (i) to cable ............... 11

4.2.2 Connecting the cable to the transmitter ................ 12

4.2.3 Calibation of pH electrode .................................. 12

4.3 Mounting the membrane body ............................ 13

4.4 Mounting the sensor .......................................... 14

4.5 Sterilization ...................................................... 14

5 Operation ........................................................ 15

5.1 Calibration of the CO2 sensor .............................. 15

6 Maintenance ................................................... 16

6.1 Inspection ........................................................ 16

6.1.1 Visual inspection .............................................. 16

6.1.2 Checking wiring and transmitter

with the pH simulator ........................................ 16

6.2 Changing the CO2 electrolyte

and membrane body ......................................... 16

6.3 Maintenance of the interior body (pH electrode) .... 17

6.4 Replacement of the interior body (pH electrode) ... 17

6.5 ISM ................................................................ 17

7 Storage ........................................................... 18

8 Productspecication ....................................... 18

8.1 Certicates ....................................................... 18

8.2 Specications ................................................... 18

9 Ordering information ........................................ 19

9.1 Sensors ........................................................... 19

9.2 Accessories ...................................................... 19

9.3 Spare parts ...................................................... 19

9.4 Transmitter ....................................................... 20

Contents

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1 Introduction

Thank you for buying the InPro™ 5000 (i) sensor

from METTLER TOLEDO.

The construction of the InPro 5000 (i) sensor employs

leading edge technology and complies with safety

regulations currently in force. Notwithstanding this,

improper use could lead to hazards for the user or

a third-party, and / or adverse effects on the plant

or other equipment.

a

Therefore, the operating instructions must be read

and understood by the persons involved before work

is started with the sensor.

The instruction manual must always be stored close at

hand, in a place accessible to all people working with

the InPro 5000 (i).

Ifyouhavequestions,whicharenotorinsufciently

answered in this instruction manual, please contact

your METTLER TOLEDO supplier. They will be glad to

assist you.

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2 Important notes

2.1 Notes on operating instructions

These operating instructions contain all the information

needed for safe and proper use of the InPro 5000 (i)

sensor.

The operating instructions are intended for personnel

entrusted with the operation and maintenance of the

sensors. It is assumed that these persons are familiar

with the equipment in which the sensor is installed.

Warning notices and symbols

This instruction manual identies safety instructions

and additional information by means of the following

symbols:

a

This symbol draws attention to safety instructions

and warnings of potential danger which, if neglec-

ted, could result in injury to persons and / or damage

to property.

h

This symbol identies additional information and

instructions which, if neglected, could lead to defects,

inefcientoperationandpossiblelossofproduction.

2.2 Intended use

InPro 5000 (i) sensors are intended solely for in

line measurement of dissolved CO2 (carbon diox-

ide), as described in this instruction manual.

Any other use, or use not mentioned here, that is in-

compatiblewiththetechnicalspecicationsisdeemed

inappropriate. The operator is solely responsible for

any damage arising from such use.

Other prerequisites for appropriate use include:

– observing the instructions, notes and requirements

set out in this instruction manual.

– observing all local safety regulations concerning

safety on work.

– observing all information and warnings in the

documentation dealing with the products used

togeth er with the sensor (housings, transmitters,

etc.).

– observing all safety regulations governing

the equipment in which the sensor is installed.

– observing the prescribed inspection and mainte-

nance intervals.

– correct care and maintenance of the unit,

according to the instruction manual.

– observing the prescribed environmental and

operational conditions, and permitted installation

positions.

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2.3 Safety instructions

– The InPro 5000 (i) sensors should be installed,

operated, maintained only by personnel familiar

with the sensor and who are qualied for such

work.

– A defective sensor must neither be install ed nor put

into service.

– Only the maintenance work described in these

operating instructions may be performed on the

sensors.

– When changing faulty components, use only

original spare parts obtainable from your METTLER

TOLEDO supplier (see section 9).

– No modications to the sensors and the

accessories are allowed. The manufacturer/

supplier accepts no responsibility for damage

causedbyunauthorisedmodications.Theriskis

borne entirely by the user.

2.4 Environmental protection

Waste electrical products should not be disposed of

with household waste. Please recycle where facilities

exist. Check with your Local Authority or retailer for

recycling advice.

a

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3 Product description

3.1 General information

The sensor InPro 5000 (i) with integrated temperature

probe is used for dissolved carbon dioxide meas ure-

ment.

The sensor has been designed for accurate and precise

measurement at low to medium carbon dioxide partial

pressure.

The sensor is sterilizable and compatible with CIP

(cleaning in place).

The sensor consists of an exchangeable pH electrode

whichisbuiltinasteelshaftandxedwithasmall

hexagonal screw.

3.2 Principles of CO2 measurement

Here is a short summary of the principle of the

potentiometric measurement of CO2 which this

sensor is based (Severinghaus). The InPro 5000 (i)

sensor employs a gas permeable silicone membrane

which is tightly stretched around a special engineered

atpHmembrane.TheCO2 gas from the sample or

process diffuses across the membrane until its partial

pressureequilibrateswithintheelectrolytelminfront

of the pH sensitive glass and the bulk of the internal

electrolyte.

The dissolved CO2 gas reacts with water to form

bicarbonate and H+ ions:

HCO3

– + H+

CO2

+

H2O

The formed H+ ions lead to a pH change of the in-

ner electrolyte which is measured with the integrated

pH electrode. Thus, the CO2 partial pressure can be

calculated using the pH and the temperature, which is

detected as well.

3.3 Scope of delivery

Each sensor is supplied together with:

– 1 bottle CO2-electrolyte 25 ml

– 1qualitycontrolcerticate

– 1inspectioncerticate3.1

(in accordance with EN 10204.3/3.1)

Membrane body and cap sleeve are not mounted.

The interior body is protected against drying out with a

wettingcap,lledwithCO2-electrolyte.

Please check completeness.

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3.4 Equipment features

VP connector

O-ring (10.77 x 2.62 mm,

silicone FDA)

Pg 13.5 threaded sleeve

Washer PTFE

Cap sleeve

Membrane body

Wetting cap

Interior body (pH electrode)

Hexagonal screw

ISM connector

O-ring (10.77 x 2.62 mm,

silicone FDA)

Pg 13.5 threaded sleeve

Washer PTFE

Cap sleeve

Membrane body

Wetting cap

Interior body (pH electrode)

Hexagonal screw

Retainer nut

InPro 5000 i

InPro 5000

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4 Preparation

4.1 Start-up

The interior body (pH electrode) is ready for calibration.

– On unpacking, check the pH electrode for

mechanical damage. Report any damage imme-di-

ately to your METTLER TOLEDO supplier.

– Remove the watering cap, cap sleeve, membrane

bodyandbrieyrinsethesensorwithde-ionized

water.

– After rinsing, the sensor should only be dapped dry

with a tissue. Do not rub the pH sensitive glass,

since this can lead to electrostatic charging and

sluggish response times.

– Check inside the pH sensitive glass for the pres-

ence of any air bubbles and remove same by gen-

tly swinging in a vertical plane (shaking down as

with a thermometer).

Beforeusingthesensorforthersttime,makesurethat

theinteriorbody(pHelectrode)iswellxedintothesteel

body. The VP connector must not be rotated in the shaft.

Ifso,theinnerparthastobexedbytighteningthesmall

hexagonal screw below the connector.

A: pH electrode

with air bubbles

will not work.

B: Correct pH electrode

with removed bubbles.

pH sensitive

glass

Ceramic

diaphragm

AB

Air

bubbles

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4.2 Calibration

Purpose of calibration: Each CO2 sensor and Interior

body (pH electrode) has its individual slope and zero

point.

4.2.1 Connecting the InPro 5000 (i) to cable

The sensor is connected to the transmitter via a VP or

AK9 cable ensuring a secure connection under harsh

industrial conditions. The robust watertight IP 68 con-

nector housing guarantees maximum process safety.

To connect the VP cable to the sensor align the notch of

the VP connector with the key in the plug. Then tightly

screw the plug to fasten the two parts.

VP cable

Plug

Notch

VP connector

AK9 cable

Plug

Notch

ISM connector

InPro 5000 InPro 5000 i

12 InPro 5000 (i) CO2 Sensor 12 mm

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4.2.2 Connecting the cable to the transmitter

For connecting the sensor please refer to the manual

of the transmitter.

4.2.3 Calibation of pH electrode

(Not needed for ISM digital InPro 5000 i)

Prepare electrode as described in section 4.1 “Start-

up”. For the analog CO2 sensor InPro 5000, we re-

commend a 2-point calibration in pH buffer pH

7.00 and pH 9.21. Before calibration, rst remove

wetting cap. Normally you start with buffer pH 7.00

for determining zero point and then buffer pH 9.21

for determining the slope. Please refer to instruction

manual of the transmitter for further details.

h

Important settings : On the transmitter, select Pt1000

as temperature sensor for the analog InPro 5000. ISM

digital InPro 5000 i does not require a temperature set-

ting.

h Important: Please do not leave the sensor in the cali-

bration buffer for longer than 1 hour. Too long exposure

may result in a high sensor drift in the process.

VP Cable

CO2 Transmitter

AK9 Cable

Min. immersion depth

> 25 >mm / 1"

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4.3 Mounting the membrane body

– After calibration of the pH electrode rinse the pH

electrode with CO2 electrolyte solution.

– Fill the new membrane body half way with CO2

electrolyte.

Make sure that all air bubbles are removed from the

membrane body. Air bubbles can be removed by care-

fully tapping on the membrane body.

– Slip the membrane body over the pH electrode

while holding the sensor in a vertical position.

The excess electrolyte will be displaced should

be wiped dry with a paper tissue.

h

Important: No electrolyte, sample media or

contamination may be present between the

membrane body and the cap sleeve. Please check

carefully.

– Carefully slip the cap sleeve over the tted

membrane body, holding the sensor in a vertical

position and screw it tight. The cap sleeve must be

clean and tight.

1/2

1/2CO2 electrolyte

14 InPro 5000 (i) CO2 Sensor 12 mm

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4.4 Mounting the sensor

Please read carefully the instruction manual of the

particular housing (InFit761-... or InTrac797) for

correct sensor installation.

The InPro 5000 (i) sensor can be mounted directly on

a top plate of a small bioreactor or in a pipe with an

inside Pg 13.5 thread and securely tightened via the

Pg 13.5 threaded sleeve.

h

Remark: Mounting upside-down or horizontaly is not

possible due to internal pH electrode. An angle of 15°

or bigger to horizontal is needed.

4.5 Sterilization

The installed sensor will be sterilized together with the

bioreactor. The InPro5000 is in-situ sterilizable or in an

autoclave. After cooling down but before inoculation a

process calibration step, as described in chapter 5, is

needed to achieve accurate measurements.

h

Remark: For best accuracy please wait one hour after

cooling down before process calibration step accor-

ding chapter 5.1.

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5 Operation

5.1 Calibration of the CO2 sensor

The InPro 5000 (i) sensor can be calibrated with CO2

gas or a CO2 / N2 gas mixture. Example: If a CO2

partial pressure of 150 mbar is expected, you can use

a gas with 15 % CO2/ 85 % N2 in nitrogen to achieve

best results. The calibration can be made in a gas or

liquid phase. For calibration in a solution you have to

wait until an equilibrium is reached. Depending on the

pH value of the solution a large amount of CO2 will

react to build bicarbonate ions before building-up a

constant partial pressure of CO2.

h

Recommendations:

–For small “benchtop” bioreactors the sensor can

be calibrated by ushing the empty or liquid

lledreactorwithaCO2 containing gas mixture.

–For larger bioreactors the gas consumption would

be too high and therefore we recommend the

calibration with the aid of the retractable

housing InTrac 797. Please operate the retract-

able housing according the instruction manual

InTrac 797 to ensure sterile operation. Additionally

thein- and outlet ofthe lower ushing chamber

mustbeequippedwithsterilelterstoavoidany

contamination with the calibration gas.

Calibration procedure:

– Sterilize CO2 sensor InPro 5000 (i) in measuring

position (membrane inside bioreactor) together

with the bioreactor.

– Sterilize lower and upper ushing chambers

of housing InTrac 797.

– After cooling down retract sensor in maintenance

position (membrane in lower chamber).

– Flush lower chamber with sterile calibration gas.

Thepressureinsidetheushingchambermustbe

known for proper calibration.

– After calibration close in- and outlet of the

lower chamber and reinsert sensor in measuring

position. Sensor is now ready for measurement.

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52 002 465 G Printed in Switzerland

6 Maintenance

6.1 Inspection

6.1.1 Visual inspection

To check the sensor, we recommend the following pro-

cedure:

– The contacts of the connector must be dry. Mois-

ture, corrosion and dirt can lead to false readings.

– Check the cable for kinks, brittle areas or tears.

– Examine the membrane foil optically for signs of

damage. The foil must be intact and clean. Dirty

membranes should be wip ed clean using a soft,

moist tissue.

h

Note:Anundulatedmembranehasnoinuenceonthe

sensor performance, assuming the membrane is intact.

– The membrane body must be replaced if the sensor

has a long response time, the reading is unstable

or subject to excessive drift, and if the sensor can-

not be calibrated with CO2 gas or the membrane

shows sign of mechanical damage.

– Check the pH sensitive glass for cracks. The dia-

phragm should show a white color. If necessary

rinse with deionized water and clean carefully with

soft paper tissue.

a

Attention! Do not use any cleaning agents containing

alcohol. This could damage the sensor or lead to

fault results.

a

Attention! The pH electrode is made of glass and

therefore fragile.

6.1.2 Checking wiring and transmitter

with the pH simulator

Necessary equipment for analog CO2 sensor

InPro 5000:

pH simulator 112 (p/n 59 906 431) and VP module

(p/n 52 120 939).

This equipment enables the testing of the VP cable

and correct wiring of the cable to the transmitter. Switch

the VP simulator to Pt 1000 position. Additionally it is

possible to check the linearity, temperature compen-

sation and quality of the input circuit (input resistance

and input current) of the CO2 transmitter.

6.2 Changing the CO2 electrolyte

and membrane body

We recommend to use a new membrane body after

each batch (see section 4.3). After each calibration

of the pH electrode (section 4.2) new CO2 electrolyte

shouldbelledinthemembranebody.

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6.3 Maintenance of the interior body (pH electrode)

If the pH electrode shows a sluggish response

time and/or insufcient sensitivity the pH sensi-

tive glass can be reactivated using a HF solution

(p/n 51 319 053). The pH sensitive glass part only

is immersed into this solution for 5 minutes, is then

immediately and thoroughly rinsed with deionized

water and then stored in CO2 Electrolyte up to the di-

aphragm for 12 hours. This procedure will make the

electrode faster but will limit the life time hardly.

6.4 Replacement of the interior body (pH electrode)

Typically after 10 – 20 sterilization cycles the pH

electrode should be replaced. Please refer to the

instruction manual of the replacement interior body

InPro 5000 (i).

6.5 ISM

DLI: Dynamic Lifetime Indicator

The DLI provides information about the remaining lifeti-

me of the interior body. As long as the DLI is above zero

daysthesystemiswithinthespeciedaccuracyaftera

calibration. If the DLI is zero the inner body needs to be

replaced.

Contributing factors for ageing:

– temperature during measurement

– number of CIP cycles

– number of SIP cycles

– number of autoclavings

The DLI is calculated in the following way.

Continuously: With the above parameters an actual

sensor stress is calculated. During the operation the

sensor load is increased. The accumulated sensor load

divided by the elapsed time is the basis of the calcula-

tion of the remaining lifetime.

TTM: Time to Maintenance

The TTM gives an indication when maintenance needs

to be performed. Maintenance includes

1. Electrolyte exchange

2. Cleanig of the inner body

3. Replacement of the membrane body

ACT: Adaptive Calibration Timer

The ACT provides information as to when the next

calibration is required to ensure measurements will

remainwithinthespeciedaccuracy.Thiscalculation

is based on the DLI information.

Calibration history

The last four calibrations and the factory calibration

data are stored in the sensor memory. These data can

be read out with a transmitter or with the iSense Asset

Suite software.

The calibration history gives valuable information re-

garding the quality of the calibration and the ageing of

the interior body.

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52 002 465 G Printed in Switzerland

7 Storage

h

For storage we recommend to ll the membra-

ne body with fresh CO2 electrolyte and rebuilt the

sensor.Thewettingcap,lledwithCO2-electrolyte, will

prevent a drying-out of the membrane.

8 Productspecication

8.1 Certicates

Each sensor is delivered with a set of 3.1certicates

(complying with EN 10204/3.1).

All wetted metal parts (sensor shaft, cap sleeve and mem-

branebody)areidentiedwithanengraved symbol corre-

sponding to the heat number onthepapercerticate

delivered with the sensor.

Each wetted metal part (sensor shaft, cap sleeve and

membrane body) is polished in order to get a surface rough-

ness lower than 0.4 µm (16 µin). This represents a rough-

ness grade number of N5 (according to ISO 1320:1992).

8.2 Specications

InPro 5000 (i)

Measurement principle Potentiometric / Severinghaus

Working conditions

Mechanical pressure resistance max. 3 bar at 25 °C

[max. 42 psi at 77 °F]

Pressure range measurement 0.2 – 2 bar absolute

[3 – 30 psi absolute]

Temperature range

(measurement)

0…60 °C [32…140 °F]

Sterilization < 130 °C [266 °F] in situ

or autoclave

Construction

Temperature signal InPro 5000 i: Digital

InPro 5000: Pt 1000

Cable InPro 5000 i: AK9

InPro 5000: VP

O-ring material Viton®, Silicone (FDA approved)

Membrane material PTFE-silicone (reinforced)

Wetted metal parts Stainless steel DIN 1.4435

similar to AISI 316 L

Dimensions

Sensor diameter 12 mm (0.47”)

Immersion length 120, 220, 320 mm

(4.7, 8.66, 12.6”)

Performance

Measuring range 10 – 1000 hPa CO2

Accuracy ± 10 %, 600 – 900 hPa

≥±10%,>900hPa

Detection limit 10 hPa CO2

Response time 90%ofnalvalue<120sec

(at 25 °C [77 °F] from air to CO2)

InPro 5000 (i) CO2 Sensor 12 mm 19

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9 Ordering information

9.1 Sensors

Designation:

InPro 5000 (i) / 12 / a (a = immersion length in mm)

Designation Order No.

InPro5000 i/12/120 30 013 606

InPro5000 i/12/220 30 019 005

InPro5000 i/12/320 30 019 006

InPro5000 i/12/420 30 748 118

9.2 Accessories

Accessory Order No.

VP cable VP6-ST/3 m 52 300 108

VP cable VP6-ST/5 m 52 300 109

VP cable VP6-ST/10 m 52 300 110

AK9 cable/1 m 59 902 167

AK9 cable/3 m 59 902 193

AK9 cable/5 m 59 902 213

AK9 cable/10 m 59 902 230

AK9 cable/20 m 52 300 204

ISMVericationKit 30 031 035

pH simulator 112 for InPro 5000 59 906 431

VP module for InPro 5000 52 120 939

9.3 Spare parts

Spare part Order No.

Membrane kit InPro 5000 52 206 055

Cap sleeve, N type 1.4435 52 201 153

Cap sleeve, P type 1.4435 52 201 154

Interior body InPro 5000 i/12/120 Kit 30 019 049

Interior body InPro 5000 i/12/220 Kit 30 019 170

Interior body InPro 5000 i/12/320 Kit 30 019 175

Interior body InPro 5000 i/12/420 Kit 30 749 397

pH buffer pH 7.00 250 ml 51 340 059

pH buffer pH 7.00 6 3 250 ml 51 340 060

pH buffer pH 9.21 250 ml 51 300 193

pH buffer pH 9.21 6 3 250 ml 51 300 194

Please check!

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9.4 Transmitter

Compatibility

InPro 5000 i InPro 5000

M400 Type 3 M400 Type 3

M800 M700 with CO2 module

Designation Order No.

M400 Type 3 52 121 350

M700 CO2 5700 i (analog only) 52 121 267

M800 Process 2-Ch 52 121 813

M800 Process 4-Ch 52 121 853

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