LI-COR Pearl Trilogy Le manuel du propriétaire

Marque: LI-COR

Modèle: Pearl Trilogy

Catégorie: Équipement musical supplémentaire

Taper: Le manuel du propriétaire

Pearl® Trilogy

Operator’s Manual

CE Marking:

This product (model number 9430) is a CE-marked product. For conformity information, contact LI-COR

Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or

distributor.

珍珠三部曲（型号9430/8500）小动物成像系统

Pearl Trilogy (Model 9430) and Pearl MSI (8500) Small Animal Imaging System

部件名称

Part Name

有毒有害物质或元素

Toxic and Hazardous Substances or Elements

铅

(PB)

汞

(Hg)

镉

(Cd)

六价铬

(Cr(VI))

多溴联苯

(PBB)

多溴二苯醚

(PBDE)

印刷电路板

(PCBs) X O O O O O

机电配件

(Electromechanical Parts) X O O O O O

光学

(Optics) OOOOOO

电缆和电线

(Cables and Wires) X O O O O O

金属部件

(Metal Parts) X O X O O O

塑料零件

(Plastic Parts) OOOOO X

电池

(Batteries) OOOOOO

本表格依据 SJ/T 11364 的规定编制

O = 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572 规定的限量要求以下

indicates that the content of the toxic and hazardous substance in all the Homogeneous Materials of the part is below the concentration limit

requirement as described in GB/T 26572

X = 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572 规定的限量要求

indicates that the content of the toxic and hazardous substance in at least one Homogeneous Material of the part exceeds the concentration limit

requirement as described in GB/T 26572

Notes on Safety

LI-COR products have been designed to be safe when operated in the manner described in this manual. The

safety of this product cannot be guaranteed if the product is used in any other way than is specified in this

manual. The Pearl® Imager is intended to be used by qualified personnel. Read this entire manual before using

the Pearl Imager.

Equipment Markings:

The product is marked with this symbol when it is necessary for you to refer to the

manual or accompanying documents in order to protect against damage to the product.

The product is marked with this symbol when a hazardous voltage may be present.

Manual Markings:

WARNING Warnings must be followed carefully to avoid bodily injury.

CAUTION Cautions must be observed to avoid damaging your equipment.

NOTE Notes contain additional information and useful tips.

IMPORTANT Information of importance to prevent procedural mistakes in the operation of the

equipment or related software. Failure to comply may result in a poor experimental

outcome but will not cause bodily injury or equipment damage.

Federal Communications Commission Radio Frequency Interference Statement

WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in

accordance with the instruction manual, may cause interference to radio communications. It has been tested

and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC

rules, which are designed to provide a reasonable protection against such interference when operated in a

commercial environment. Operation of this equipment in a residential area is likely to cause interference in

which case the user, at his own expense, will be required to take whatever measures may be required to

correct the interference.

Page 4

Notice

The information contained in this document is subject to change without notice. LI-COR MAKES NO

WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO

THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.

LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection

with the furnishing, performance, or use of this material.

This document contains proprietary information that is protected by copyright. All rights are reserved. No part

of this document may be photocopied, reproduced, or translated to another language without prior written

consent of LI-COR, Inc.

Printing History

Publication Number 977-19834

Printed: June 2016

Revised: 2022-03-22

change. LI-COR, Pearl, In-Cell Western, IRDye, Odyssey, and Image Studio are registered trademarks of LI-COR,

Inc. in the United States and other countries. All other trademarks belong to their respective owners. The

Odyssey Infrared Imaging Systems and IRDye reagents are covered by U.S. patents, foreign equivalents, and

patents pending.

Global Headquarters

4647 Superior Street • Lincoln, NE 68504

Toll free: 800-645-4267 • Fax: +1-402-467-0819 • [email protected] •licor.com/bio

Regional Offices

LI-COR Biosciences GmbH

Siemensstraße 25A • 61352 Bad Homburg • Germany

Phone: +49 (0) 6172 17 17 771 • Fax: +49 (0) 6172 17 17 799 • [email protected]

LI-COR Biosciences UK Ltd.

St. John’s Innovation Centre • Cowley Road

Cambridge • CB4 0WS

United Kingdom • Phone: +44 (0) 1223 422104 • Fax: +44 (0) 1223 422105 • [email protected]

LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors

Page 5

Chapter 1: Installation and Safety Information

Safety Considerations 9

Laser Safety 9

Safety Interlocks 10

Placement in the Laboratory 11

Ambient Laboratory Conditions 11

Instrument Ventilation 12

Room Ventilation 12

Space Requirements 12

Level the Imager 12

Moving the Imager 12

Anesthesia Safety Considerations 13

Oxygen Safety Considerations 13

Chemical Safety 13

Use of DMSO and Other Strong Solvents 13

Electrical Considerations 15

Power Cords and Disconnecting Power 15

Fuse Replacement 15

Chapter 2: System Overview

External Panels and Controls 17

Front Panel 17

Right-side Panel 18

Rear Panel 19

Imaging Drawer 19

Imaging Drawer Safety 19

Installing the Standard Imaging Bed 20

Computer Connections and Networking 24

Image Studio™ Software Features 25

Optical System Description 25

Near-Infrared Detection 27

Bioluminescent Detection 27

Overview of Infrared In Vivo Imaging 27

References 29

Pearl® Trilogy

Imaging

System

Table of Contents

Page 6

Chapter 3: Connecting an Anesthesia System

Installing the Rotameter and Charcoal Filter 31

Connecting an Alternate Scavenging System 33

Connecting an Anesthesia System 33

Checking the Imaging Bed Connections 33

Starting the Anesthesia Gas Flow 35

Stopping the Anesthesia Gas Flow 35

Flow Path for Anesthesia Gas 35

Chapter 4: Operation

Overview 37

Cleaning the Imaging Bed 37

Setting the Heater Plate Temperature 38

Starting the Anesthesia Gas 38

Placing Mice on the Imaging Bed 40

Using Restraints 40

Using a Mouse Blanket 41

Imaging Tips 41

Hair vs. Shaved Animal 41

Diet Considerations 41

Mouse Recovery Procedure 42

Chapter 5: Routine Maintenance

Routine Maintenance 45

Cleaning the Fan Filter 45

Nose Cone Replacement 46

Replacing Heater Plate Contact Pins 46

Desiccant Replacement 47

Pearl® Imager Installation Instructions 47

Front Panel Warning Light Indicates an Error 49

Resetting the Instrument 49

Obtaining Technical Support 49

Exporting ImageStudio Software Log Files 49

Chapter 6: Pearl Docking Station

Description 51

Assembling the Pearl Docking Station 52

Docking Station Operation 56

Routine Maintenance 58

Docking Station Specifications 58

Chapter 7: Pearl Clean Box

Page 7

Description 59

Installation 59

Connecting a HEPA Filter to the Docking Station 59

Connecting a HEPA Filter to the Pearl® Imager 60

Nose Cone Installation and Replacement 60

Sanitizing the Pearl Clean Box 61

Clean Box Operation 61

Maintenance of the Clean Box Window 62

Scratches 62

Smudging 62

Chemicals 63

Cleaning Procedure 63

Replacing Heater Plate Contact Pins 63

Chapter 8: Appendices

Specifications 65

Embedded Firmware Source Code 66

Page 8

Chapter 1: Installation and Safety Information

Safety Considerations

Laser Safety

The Center for Devices and Radiological Health (CDRH) was established in October, 1982, by the

U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical

devices and radiological health.

Manufacturers of products subject to performance standards under the Radiation Control for Health

and Safety Act of 1968 are required to furnish various reports to the CDRH.

The Pearl Imager is certified as a Class I laser product. This means that hazardous laser radiation is

not emitted outside the instrument. Radiation emitted inside the Pearl Imager is confined within

protective housings and external covers. A series of safety interlocks ensures that the laser beam

cannot escape during any phase of user operation.

Two lasers emit at 785 nm and the other two at 685 nm. The 785nm lasers have a peak power rating

of 1 watt. The 685nm lasers have a peak power rating of 0.5 watt. The 685 nm laser emits visible laser

radiation - direct exposure to either beam may cause eye damage. Laser radiation from each laser

uniformly illuminates the target area, which is the imaging bed (Figure 2-5) in the imaging drawer.

The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation

performance standard 21, Code of Federal Regulations Chapter 1, Sub-chapter J). Compliance for

products marketed in the United States is mandatory. This product complies with CDRH radiation

performance standard 21 CFR Chapter 1, Sub-chapter J. Ce produit est conforme à la performance de

rayonnement CDRH chapitre CFR norme 1 Sous-chapitre J. The label that must be attached to laser

products marketed in the United States is Figure 1-1 and is located on the rear panel of the Pearl

(Figure 1-2), indicating compliance with CDRH regulations.

WARNING: Use of controls or adjustments or performance of procedures other than those specified

herein may result in hazardous radiation exposure.

AVERTISSEMENT: L'utilisation des commandes ou le réglage ou la performance des procédures

autrement qu'il a été indiqué dams le présent texte peut résulter en de dangereuses expositions aux

radiations.

Pearl® Trilogy

Imaging

System

Page 9

Chapter 1: Installation and Safety Information

Figure 1-1. CDRH and IEC 60825-1:2007 regulation

compliance label. See Power Cords and Disconnecting

Power on page15 for more information about

disconnecting power before maintenance or servicing.

Figure 1-2. Location of CDRH compliance label on the

Pearl®ImagingSystem. Note: The appearance of your

instrument's back panel may vary slightly from the one

shown.

DANGER - CLASS 4 VISIBLE AND INVISIBLE LASER

RADIATION WHEN OPEN AVOID EYE OR SKIN

EXPOSURE TO DIRECT OR SCATTERED RADIATION

DANGER VARNING

DANGER - APPAREIL LASER

DE CLASSE 4. RAYONNEMENT

LASER VISIBLE ET INVISIBLE

EN CAS D’OVERTURE. EVITER

L’EXPOSITION DE L’OEIL OU DE

LA PEAU AU RAYONNEMENT

DIRECT OU DIFFUS.

VARNING – KLASS 4 SYNLIG

OCH OSYNLIG LASERSTRÅLNING

NÄR ENHETEN ÄR ÖPPEN.

UNDVIK EXPONERING AV ÖGON

OCH HUD I DIREKT ELLER

REFLEKTERAD STRÅLNING.

Figure 1-3. Class 4 laser warning label.

Safety Interlocks

Where there is a potential for class 4 laser radiation behind a given panel, the label in Figure 1-3 is

affixed to the panel. The label shown in Figure 1-3 is affixed to the Pearl Imager in two locations; one

on the rear lower access panel and one on top of the camera hood (Figure 1-4).

Figure 1-4. Location of class 4 laser warning labels located (a) on the camera hood under the top panel of the Pearl

Imager instrument enclosure, and (b) on the exterior of the rear lower access panel.

Page 10

Pearl® Trilogy Imaging System

The Pearl Imager has two safety interlocks that prevent exposure to laser radiation when the Pearl

imaging drawer is opened during operation. Do not attempt to defeat these interlocks. The drawer

interlocks are located in the back of the instrument behind the rear lower access panel, which is also

protected by interlocks. This second set of interlocks prevents exposure to laser radiation if the rear

lower access panel is removed during operation. These interlocks are also located behind this back

access panel (Figure 1-5). Do not attempt to defeat these interlocks (Figure 1-6).

Figure 1-5. Location of laser interlock warning label behind the rear lower access panel.

DANGER - CLASS 4 VISIBLE

AND INVISIBLE LASER

RADIATION WHEN OPEN

AND INTERLOCKS

DEFEATED AVOID EYE

OR SKIN EXPOSURE TO

DIRECT OR

SCATTERED

RADIATION

VARNING – KLASS 4 SYNLIG

OCH OSYNLIG LASERSTRÅLNING

NÄR ENHETEN ÄR ÖPPEN OCH

FÖRREGLING ÅSIDOSATTS.

UNDVIK EXPONERING AV

ÖGON OCH HUD I

DIREKT ELLER

REFLEKTERAD

STRÅLNING.

DANGER - APPAREIL LASER DE

CLASSE 4. RAYONNEMENT LASER

VISIBLE ET INVISIBLE EN CAS

D’OVERTURE ET LORSQUE LE

DISPOSITIF DE SECURITE EST

NEUTRALISE. EVITER L’EXPOSITION E L’OEIL

OU DE LA PEAU AU RAYONNEMENT

DIRECT OU DIFFUS.

Figure 1-6. Laser interlock warning label.

Placement in the Laboratory

The Pearl Imaging System weighs approximately 25 kg (55 lb). The instrument should be placed on a

laboratory bench that is sufficiently sturdy to bear its weight. The bench should also be able to bear

the weight of the computer if the computer is to be installed beside the Pearl Imaging System

instrument.

Ambient Laboratory Conditions

Place the Pearl Imaging System away from external heat sources (furnaces, windows, etc.). Additional

heating can cause high temperatures within the enclosure. The heater plate in the Pearl Imaging

System imaging bed cannot cool the imaging bed if ambient temperature is above the set point for

the imaging bed heater plate.

Page 11

Chapter 1: Installation and Safety Information

Place the instrument away from sinks or other sources of water that pose a shock hazard.

Recommended operating conditions are 15-35 °C and a dew point not greater than 22 °C to prevent

condensation on the laser assemblies, which are cooled during operation.

Instrument Ventilation

The air intake fan on the back of the Pearl®ImagingSystem (Figure 2-4) is used to cool the internal

electronics and maintain fresh air circulation throughout the internal volume of the instrument.

Fresh air circulation prevents an internal buildup of anesthesia gas or oxygen. The air intake is

filtered and the filter should be periodically cleaned (Routine Maintenance on page45). Make sure

there is plenty of room behind the instrument (Figure 2-5) so that the air intake is not blocked and

the filter is easily accessible for cleaning.

Room Ventilation

A room with a minimum of 15 air changes per hour (non-recirculating) is required when using

isoflurane anesthesia gas.

Space Requirements

The Pearl Imaging System instrument requires an area approximately 41 cm W (16") x 41 cm D (16")

x 66 cm H (26"). Depth with the imaging drawer open is 63.5 cm (25”).

Level the Imager

Level the instrument to prevent the imaging drawer from scraping the lab bench or table in front of

the instrument and to keep the instrument from tilting.

The instrument can be leveled by turning the threaded metal inserts located in each of the 4 plastic

feet (Figure 1-7).

Figure 1-7. Turn feet to adjust height.

Moving the Imager

LI-COR recommends that you do not move your imager. Instead, please contact LI-COR Technical

Support for options.

LI-COR cannot be held responsible for any damage incurred while moving, transporting, or

shipping the Pearl Imager, even if an existing warranty and/or service contract applies. LI-COR

cannot be held responsible for any injury incurred while moving, transporting, or shipping

the Pearl Imager.

Page 12

Pearl® Trilogy Imaging System

Anesthesia Safety Considerations

WARNING: Isoflurane gas is a human inhalation hazard that needs to be ventilated away

from workers. It can cause coughing, sore throat, dizziness, drowsiness, headache, and

unconsciousness. Pregnant women should not work in an environment where there is any

potential isoflurane risk.

AVERTISSEMENT:

Le gaz isoflurane est un danger pour le système respiratoire humain et il doit être dispersé par

ventilation loin des ouvriers. Il peut provoquer la toux, la gorge endolorie, le vertige, la

somnolence, le mal de tête, et l'inconscience. Les femmes enceintes ne devraient pas travailler

dans un environnement où il existe un danger d'inhalation d'isoflurane.

Oxygen Safety Considerations

Oxygen gas is a colorless, odorless, oxidizing gas. If O2is used as a carrying gas for the anesthesia gas,

the chief physical hazard associated with the release of oxygen is its oxidizing power, which can

greatly accelerate the burning rate for combustible materials. Keep the room well ventilated with

fresh air by observing the room ventilation recommendations above.

Chemical Safety

LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory

procedures be followed at all times. Be aware of the hazards associated with any chemical before you

begin work.

The LI-COR®Pearl Imaging System should not be used with any radioactive materials.

Use of DMSO and Other Strong Solvents

CAUTION: Strong solvents, including DMSO, will permanently discolor the imaging bed

surface. Avoid all contact. In addition, any IRDye®products in DMSO will incorporate into

the surface of the imaging bed and will be visible in images.

ATTENTION:

Les dissolvants forts, y compris DMSO, décoloreront de manière permanente la surface du lit

d'image. Évitez tout contact. En outre, tous les produits d'IRDye dans DMSO incorporeront à

la surface du lit et seront évidents dans les images.

Page 13

Chapter 1: Installation and Safety Information

Figure 1-8. Caution labels for DMSO and other strong solvents.

Whenever possible, inject the animal on a separate surgical bed and clean off any exterior dye

product on the animal before transferring the animal onto the Pearl Imaging System imaging bed. In

the event that the imaging bed becomes contaminated with dye, wipe with Nair®hair remover and

thoroughly clean and dry before use.

Page 14

Pearl® Trilogy Imaging System

Electrical Considerations

Power Cords and Disconnecting Power

The Pearl®ImagingSystem is equipped with a 3-wire grounding-type plug. This plug will only fit

into a grounding-type outlet. This is a necessary safety feature. If you are unable to insert the plug

into the outlet, you will need to replace the outlet. Do not defeat the purpose of the grounding-type plug.

Do not locate the Pearl Imaging System where the power cord will be walked on or exposed to water

or chemical spills.

The Pearl Imaging System draws a maximum of 4 amperes at 120V (see Specifications on page65). If

an extension cord is used, make sure the total of the ampere ratings on the instruments plugged into

the extension cord does not exceed the extension cord ampere rating. Also make sure the total

amperage of instruments plugged into the wall outlet does not exceed the amperage capacity for the

outlet (usually 15 or 20 amperes in the United States).

Disconnect power before maintenance or servicing.

Débrancher le pouvoir avant d'entretenir.

See Figure 1-1 on page10 for the location of these statements on the product's regulation compliance

label.

Fuse Replacement

There are no user replaceable fuses in this instrument.

Page 15

Page 16

Chapter 2: System Overview

External Panels and Controls

Front Panel

Power on/off button

Image acquisition 'in

progress' indicator (shown

unlit)

Error condition indicator

(unlit)

Scavenging charcoal filter

Rotameter indicating flow rate

of anesthesia gas

Rotameter flow control valve

Imaging drawer open/close

button

Imaging drawer (closed)

Figure 2-1. Front panel features and controls of the Pearl Imager.

Power On/Off Button: Pressing this button momentarily turns the instrument on and off. Pressing

the button for 5 seconds or more cuts power to the instrument (Routine Maintenance on page45).

While the instrument is starting up or shutting down, the blue indicator light in the power button

blinks. When the blue indicator light is continuously on, the instrument is ready for operation.

Pearl® Trilogy

Imaging

System

Page 17

Chapter 2: System Overview

Note: Instruments with firmware version 1.3.1 and earlier will shut down if you press the power

button, even if the image acquisition light is on. On instruments with newer firmware, the red

error indicator will illuminate if you press the power button while the image acquisition indicator

is on. Hold down the power button to force a shut down.

Green Image Acquisition Light: This light blinks during image acquisition and is continuously on

at other times, as long as communication with Image Studio™Software is maintained. The light is

off only if communication is lost or Image Studio Software is closed.

Red Error Light: The error light illuminates to indicate that a particular process could not be

completed. See Routine Maintenance on page45.

Rotameter: The rotameter provides flow control for anesthesia gas, as well as inlet connectors to

receive anesthesia gas from the SmartFlow Anesthesia System or a user-supplied anesthesia system.

Scavenging Charcoal Filter: The charcoal filter removes isoflurane gas from the exhaust air stream.

Imaging Drawer Button: This button opens and closes the imaging drawer in normal operation.

The drawer handle on the outside of the drawer can be used to open or close the drawer in cases of

power loss or emergency.

Imaging Drawer: The imaging drawer moves the mouse into position under the camera for

imaging. The imaging drawer contains the imaging bed, which provides heat and anesthesia gas for

the mouse.

Right-side Panel

Figure 2-2. Right-side panel features and controls of the

Pearl Imager.

Figure 2-3. Close up of the Pearl control module showing

the power receptacle and Ethernet connectors (use only

the supplied Ethernet cable). Do not use any other

connector on this panel. Note: The appearance of your

instrument's back panel may vary slightly from the one

shown.

Page 18

Pearl® Trilogy Imaging System

Rear Panel

Rotameter in

storage position

Air fan inlet

Lower rear access

panel

Figure 2-4. Rear panel showing air inlet fan. Keep the inlet unblocked during operation and accessible for routine filter

cleaning (Routine Maintenance on page45).

Imaging Drawer

Figure 2-5. The drawer front tilts forward and shifts

downward automatically when opened, making the

removable imaging bed easier to access.

Imaging Drawer Safety

Be careful not to get your fingers caught between the Pearl®ImagingSystem and the imaging drawer

when the drawer closes. The drawer is specially designed to stop if a certain level of resistance is

detected, so no harm should result, but the experience may be startling.

Page 19

Chapter 2: System Overview

During an unexpected power loss, the drawer button will not function. This may leave a mouse

stranded inside the Pearl Imaging System, or it may result in the imaging drawer being left partially

open or closed. In any case, the drawer can be opened or closed by pulling or pushing on the drawer

handle on the drawer front.

The imaging drawer should typically be opened and closed using the drawer button, but the

drawer handle can be used to manually open or close the drawer. The drawer handle is

especially useful in case of power loss or emergency.

Installing the Standard Imaging Bed

1) Make sure the imaging bed and imaging drawer are clean and dry (Routine Maintenance on

page45) before starting.

2) Make sure a nose cone plug is in place in the nose cone on the imaging bed.

Figure 2-6. Anesthesia gas connectors on the imaging

drawer are closed when the imaging bed is removed but

open immediately when the imaging bed is locked in

place.

WARNING: If anesthesia gas is flowing

to the Pearl Imager, gas will start flowing

to the nose cone immediately after the

imaging bed is locked in place. Make sure

the nose cone plug is in place before

installing the imaging bed.

AVERTISSEMENT:

Si le gaz d'anesthésie coule dans Pearl, le

gaz commencera à couler dans le cône de

nez dès que le lit d'image est installé en

place fermement. Assurez-vous que la

prise de cône de nez est en place avant

d'installer le lit d'image.

Figure 2-7. If the system is pressurized with anesthesia gas, the nose cone plug should be in place before the

imaging bed is locked into the imaging drawer to minimize operator exposure to anesthesia gas.

Page 20

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LI-COR Pearl Trilogy Le manuel du propriétaire

Marque: LI-COR

Modèle: Pearl Trilogy

Catégorie: Équipement musical supplémentaire

Taper: Le manuel du propriétaire

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