Pearl® Imager Accessories
Sanitizing the Pearl Clean Box
CAUTION: Do not sterilize the Pearl Clean Box in an autoclave.
Damage will result.
ATTENTION:
Ne stérilisez pas la boîte propre de Pearl dans un autoclave. Des endommages résulteront.
There are two considerations when sanitizing the Pearl Clean Box. The most important is to sanitize
the interior of the Clean Box to protect the health of the immunocompromised mouse. The second is
to keep the Clean Box window and imaging bed clear of any dirt, smudges, or foreign debris that
may be detected during infrared imaging.
Before cleaning, read Maintenance of the Clean Box Window on page12. For the initial cleaning,
use Heavy Duty Optical Wipes with a solution of 0.5% chlorine bleach . Clean the imaging bed
surface, interior surface of the Clean Box lid, and interior surface of the Clean Box window. Check
for smudges on the interior of the window. If necessary, perform a final cleaning using the LI-COR®
Optical Tissue. Smudges on the window could be visible in all images acquired using the Clean Box,
so clean thoroughly and carefully.
With infrared imaging, residue on the imaging bed surface from previous image acquisitions may be
visible in new image acquisitions. If necessary, between image acquisitions, clean just the imaging
bed surface with 0.5% chlorine bleach . Ethanol or isopropanol can be also used as a cleaning
solution for applications where chlorine bleach is not required. If necessary, clean any fingerprints or
smudges from the exterior surface of the window as described in Maintenance of the Clean Box
Window on page12.
CAUTION: Strong solvents, including DMSO, will permanently discolor the imaging bed
surface. Avoid all contact. In addition, any IRDye®products in DMSO will incorporate into
the surface of the imaging bed and will be visible in images.
ATTENTION:
Les dissolvants forts, y compris DMSO, décoloreront de manière permanente la surface du lit
d'image. Évitez tout contact. En outre, tous les produits d'IRDye dans DMSO incorporeront à
la surface du lit et seront évidents dans les images.
Whenever possible, inject the animal on a separate surgical bed and clean any exterior dye
product on the animal before transferring the animal onto the Pearl Imager Clean Box. In the
event that the Clean Box becomes contaminated with dye, wipe with Nair®hair remover and
thoroughly clean immediately and dry before use.
Clean Box Operation
1) Open the Clean Box and clean the imaging bed surface with 0.5% chlorine bleach as described
above. To open, swing the latch down 90° until horizontal, rotate 180° counter clockwise, and
swing the latch up before lifting the Clean Box lid.
2) Close the Clean Box, place the Clean Box on the Pearl Docking Station and lock it in place.
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